Staining

Question 1

What is the stain used for starch

Answer 1

Iodine solution

Question 2

What colour does the iodine solution become

Answer 2

Blue-black

Question 3

What stain is used for gram staining

Answer 3

Crystal violet

Question 4

What colour does crystal violet turn

Answer 4

Purple

Question 5

What is methylene blue used to stain for

Answer 5

Nuclei

Question 6

What colour does methylene blue turn

Answer 6

Blue

Question 7

What is the purpose of staining

Answer 7

Staining makes it easier to distinguish different cell structures, it also increases the contrast

Question 8

How can you apply a stain if a specimen is already mounted

Answer 8

By placing the solution at one end of the cover slip and then filter paper at the other end, it will then move along by capillary action staining the cells.

Question 9

What are viable stains

Answer 9

Stains that are applied to living material

Question 10

What are non viable Staines

Answer 10

Stains that are applied to dead material

Question 11

What is differential staining

Answer 11

It involves a series of stains and includes a stage which differentiates between different groups or different parts of the of the cell.

Question 12

What is an example of differential staining

Answer 12

U add a stain causing one colour to show up. You then add another stain fixing it to only one type out of the twos features. You then add alcohol removing the stain that isn’t fixed. You then add a stain that only shows up when the first stain isn’t present. Allowing you to differentiate between the two types.