spirochetes Flashcards

1
Q

what is the unique morphological future of Spirochete

a. motility without flagella
b. corksrew motility
c. rotary movement
d. helically coiled

A

Spirochetes

  • helically coiled and motile through Endoflagella / axial filamements
  • UNIQUE MORPHOLOGICAL FEATURE : MOTILITY WITHOUT FLAGELLA because of endoflagella
  • they exhibit corksrew like rotary movement
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2
Q

spirochetes do not stain well with what stain

a. gram stain
b. giemsa stain
c. both

A

spirochetes do not stain well with GRAM STAIN

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3
Q

what spp of Borrelia is agent of lousse - borne epidemic RELAPSING FEVER

a. Borrelia recurrentis
b. Borrelia burgdorferi
c. borrelia burgdorferi sensu stricto

A

borrelia recurrentis
- relapsing fever

borrelia burgdorferi
- lyme disease or lyme borreliosis

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4
Q

what spp of borrelia is the only human pathogenic spp found in north america

a. Borrelia recurrentis
b. Borrelia burgdorferi
c. borrelia burgdorferi sensu stricto

A

borrelia recurrentis
- relapsing fever

borrelia burgdorferi
- lyme disease or lyme borreliosis

borrelia burgdorferi sensu stricto
- only human pathogenic spp

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5
Q

what culture media to be used in Borrelia recurrentis

a. human blood bilayer (HBT)
b. MAC
c. BSK II medium
d. mod. kelly medium

A

ans: c and d

culture media: BSKII medium or mod. kelly medium

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6
Q

most common anthropod borne disease in US

a. borrelia burgdorfer
b. borrelia recurrentis
c. leptospira interogans
d. erysipelothriz rhusiopathiae

A

borrelia burgdorferi – lyme disease

- most common anthropod borne disease in US

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7
Q

what is spp ECM / erythema chromium migrans belong

a. borrelia recurrentis
b. borrelia burgdorferi
c. leptospiera interrogans
d. treponema pallidum

A

Borrelia burgdorferi

- ECM : ERTHYEMA CHROMIUM MIGRANS : a red popular rash ( bulls eye rash) that spread into a larger lesion

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8
Q

what stages og infection in borrelia borgderferi is lyme disease

a. stage 1
b. stage 2
c. stage 3

A

stage of infection
stage 1 - EM rash
stage 2 - blood dissemination
stage 3 - LYME DISEASE

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9
Q

Gold standard in Lyme disease testing

a. widal’s test
b. western blot
c. weil’s felix test

A

Western blot
- borrelia
{screening test for borelia : elisa }

widal’s test
- salmonella

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10
Q

treponema is difficult to visualize with a bright field microscope but can be observed with __________

a. phase contrast microscope
b. dark field microscope
c. fluorescent microscope

A

DARK FIELD MICROSCOPE
treponema

borrelia - bright field

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11
Q

what is the reproduction of treponema

a. binary fission
b. multiple fission
c. both
d. nota

A

MULTIPLE FISSION - REPRODUCTION O FTREPONEMA

Borrelia - multiply by binary fission

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12
Q

how many hours treponema visible in whole blood or plasma

a. atleast 72 hours
b. 8 hours
c. 12 hours
d. 24 hours

A

it remains visible in whole blood or plasma for atleast 24 hours , which is POTENTIAL IMPORATNCE IN BLOOD TRANSFUSION

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13
Q

what spirochetes can stain in gram stain

a. leptospira
b. borrelia
c. treponema
d. aota

A

spirochetes DO NOT STAIN WELL AT GRAM STAIN

Borrelia - stains well with GIEMSA

Leptospira - stains fainltywith ANILINE DYE

TREPONEMA - fail to stain or STAIN VERY POORLY WITH GRAM STAIN AND GIEMSA STAIN

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14
Q

what disease is also known as GREAT IMMITATOR

a. hiv
b. syphilis
c. diabetes
d. anemia

A

Syphilis ( French disease / Italian disease / The Great Pox )

  • it is a disease of the BLOOD VESSELS and PERIVASCULAR AREAS
  • it is also known as the GREAT IMMITATOR because it can copy and assume many clinical manifestation
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15
Q

what stages of syphilis ha sthe appearance of HUNTERIAN OR HARD CHANCRE

a. primary
b. secondary
c. tertiary
d. latrent

A

Primary syphilis

  • it is characterized by the appearance of HUNTARIAN OR HARD CHANCRE. which is infectious primary lession that is painless and usually seen at the site of innoculation (most commonly is in genitalia)
  • it develops at 10 to 90 days after infection
  • no systmeic signs and symptoms

SOFT chancre - haemophilus ducreyi

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16
Q

what stage of syphilis is highly infectious

a. primary
b. secondary
c. tertiary
d. latrent

A

Secondary syphilis

  • it develops 2 to 12 weeks after the appearance of chancre
  • all lessions that are observed seein in this phase is highly infectious
17
Q

what stage of syphilis has a diagnosis is done by serological test only

a. primary
b. secondary
c. tertiary
d. latrent

A

tertiary syphilis

  • disease are already subclinical
  • diagnosis can be made only by serologic test
18
Q

what stains to be used in Treponema spp

a. gram stain
b. giemsa stain
c. Fontana tribondeau stains
d. levaditi’s stain

A

treponema
- most spp stains poorly at gram and giemsta stain

microscopy
stains used : Levaditi’s stain and Fontana tribondeau stain

19
Q

what test is used for the detection of CONGENITAL SYPHILIS

a. rapid plasma reagin (RPR)
b. PCR
c. Western blot
d. venereal disease research laboratory (VDRL) test

A

western blot
- is used for the detection of CONGENITAL SYPHILIS

PCR
- used for NEUROSYPHILIS DETECTION (aids patients)

20
Q

what test is used for neurosyphilis detection

a. rapid plasma reagin (RPR)
b. PCR
c. Western blot
d. venereal disease research laboratory (VDRL) te

A

western blot
- is used for the detection of CONGENITAL SYPHILIS

PCR
- used for NEUROSYPHILIS DETECTION (aids patients)

21
Q

what test does not require the heating of the serum and not recommended for csf

a. rapid plasma reagin (RPR)
b. PCR
c. Western blot
d. venereal disease research laboratory (VDRL) test

A

RPR

  • does NOT require HEATING of the serum
  • not recommended for CSF

VDRL

  • recommended for the diagnosis of neurosyphilis using CSF specimens
  • it must be FRESHLY PREPARED and the patient’s serum must be HEATED AT 56 DEGREE CELCIUS FOR 30 MINUTES
22
Q

WHICH of the following is false positive in RPR AND VDRL test result

a. old age
b. Systemic Lupus Erythematosus
c. rheumatic fever
d. infectious mononucleosis

A

cause of FALSE POSITIVE RPR AND VDRL TEST

  • old age
  • pregnancy
  • hepatitis
  • rheumatic fever
  • SLE
  • IM
23
Q

which of the following antibodies in treponema pallidum is used to monitor the treatment of the syphilis

a. treponemal test
b. non treponemal test

A

non treponemal test / non specific test - Screening test
- it is used to MONITOR THETREATMENT of the syphilis

Treponemal test / Specific test - Confirmatory test

24
Q

which of the following has a causative agent of venereal syphilis

a. treponema pallidum subsp pallidum
b. treponema pallidum subs pertenue
c. treponema pallidum subsp endemicum
d. treponema pallidum subsp carateum

A

treponema pallidum subsp pallidum

- C.A : venereal syphilis

25
Q

which of the following has a causative agent of pinta

a. treponema pallidum subsp pallidum
b. treponema pallidum subs pertenue
c. treponema pallidum subsp endemicum
d. treponema carateum

A

treponema carateum

- C.A : pinta or carate

26
Q

which of the following has a causative agent of Bejel

a. treponema pallidum subsp pallidum
b. treponema pallidum subs pertenue
c. treponema pallidum subsp endemicum
d. treponema carateum

A

treponema pallidum subsp . Endemicum

  • C.A: endemic non venereal syphilis or BEJEL
  • transmitted by direct contact with active lession and CONTAMINATEES FINGERS AND UTENSILS
27
Q

which of the following has a causative agent of YAWS

a. treponema pallidum subsp pallidum
b. treponema pallidum subs pertenue
c. treponema pallidum subsp endemicum
d. treponema carateum

A

Treponema pallidum subsp pertunue

- C.A : yaws or frambesia tropics

28
Q

which of the following can cause VINCENT’S DISEASE

a. treponema pallidum subsp pallidum
b. treponema pallidum subs pertenue
c. treponema denticola
d. treponema carateum

A

Treponema sacranskii, T. denticola and T. pectinovorum
- isolated form individuals with GINGIVITIS OR PERIODONTAL DISEASE
may cause acute necrotizing ulcerative gingivitis knowns as VINCENT’S DISEASE