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bacteriology.. > 2 miscellaneous gram nega bacilli .. > Flashcards

2 miscellaneous gram nega bacilli .. Flashcards

1
Q

what species of gram negative bacilli uses RABBIT’S OR HORSE blood agar

a. haemophilus
b. helicobacter
c. staphylococcus
d. campylobacter

A

haemophilus
- RABBIT OR HORSE blood agar are commonly used for detecting hemolysis by hemolysin producing strains of haemophilus unable to grow in 5% SBA

  • though they are “blood lovers”, most species cannot grow on pure BAP
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2
Q

x factor is produced by?

a. potato extract
b. yeast extract
c. breakdown of hemoglobin

A

X factor

  • hematin/ protoporphyrin IX / hemin
  • heat stable
  • produced by the breakdown of hemoglobin
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3
Q

v factor is produced by

a. potato extract
b. yeast extract
c. breakdown of hemoglobin

A

V factor

  • cofactor 1 or NAD
  • heat labile
  • produced by certain bacteria
  • potato or yeast extract
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4
Q

which of the following species of haemophilus is the first bacterial genome to be sequenced and made public

a. haemophilus influenzae
b. haemophilus aegypticus
c. h. ducreyi
d. h. parahaemolyticus

A

haemophilus influenzae

- 1ST BACTERIAL GENOME TO BE SEQUUENCED AND MADE PUBLIC

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5
Q

which of the following species of haemophilus is the only xylose positive

a. haemophilus influenzae
b. haemophilus aegypticus
c. h. ducreyi
d. h. parahaemolyticus

A

haemophilus influenzae

-only xylose positive

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6
Q

agent of epiglottis

a. haemophilus influenzae
b. haemophilus aegypticus
c. h. ducreyi
d. h. parahaemolyticus

A

haemophilus influenzae

  • PFEIFFERS BACILLUS
  • agent of EPIGLOTTIS
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7
Q

which of the following haemophilus causes PINK EYE

a. haemophilus influenzae
b. haemophilus aegypticus
c. h. ducreyi
d. h. parahaemolyticus

A

haemophilus influenzae biogrp aegypticus

  • causes pink eye. a very contagious conjunctivitis
  • has a been isolated as the cause of BRAZILLIAN PURPURIC FEVER
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8
Q

which of the following serotype of haemophilus influenzae is most frequently encountered serotypes

a. haemophilus influenzae a
b. haemophilus influenzae b
c. haemophilus influenzae c
d. haemophilus influenzae d
e. haemophilus influenzae e
f. haemophilus influenzae f

A

HiB / haemophilus influenzae B

`

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9
Q

which of the following on gram stain is school of fish

a. h. influenzae
b. h. aegyptcus
c. h. ducreyi
d. h. parainfluenzae

A

haemophilus ducreyi

  • infective agent : CHANCROID. a soft chancre venereal disease
  • requires addition growth factors and special media
  • gram stain : school of fish
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10
Q

which of the following species can be mistaken as group a streptococcus on BAP

a. h. ducreyi
b. h. influenzae
c. h. haemolyticus
d. h. parainfluenzae

A

haemophilus haemolyticus

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11
Q

what media is preferred to use in haemophilus

a. cap
b. bap
c. thio
d. BHI

A

CAP is the preferred medium for Haemophilus because it contains X and V factor

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12
Q

rapid direct identification test for the capsular antigen of H. influenzae

a. porphyrin test
b. neufeld quallung reaction
c. cap

A

neufeld quallung reaction
- rapid direct identification test of the capsular antigen for H. influenzae

porphyrin test
- for identifying heme producing species of haemophilus

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13
Q

what is test reagent to be used in porphyrin test

a. horsed blod
b. rabbits blood
c. kovacs reagent

A

kovacs reagent ( p -dimethylaminobenzaldehyde)

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14
Q

which of the selective media to be used in H. influenzae for respiratory secretions of patients with cystic fibrosis

a. cap wiith 1% IsoVitaleX
b. nairobi biplate medium
c. horse’s blood bacitracin agar

A

h. influenzae - horse’s blood bacitracin agar
h. aegypticus - CAP with 1% IsoVitaleX or Vitox
h. ducreyi - sleective Nairobi biplate medium

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15
Q

which of the following organism is positive for X and v factor

i. h. influenzae
ii. h. aegypitus
iii. h. haemolyticus
iv. h. ducreyi

a. i, ii
b. ii only
c. i, ii, iii, iv
d. i, ii, iii

A

X and V {HAI} POSITIVE

h. HAEMOLYTICUS
h. AEGYPTICUS
h. INFLUENZAE

h. ducreyi is positive only on X factor

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16
Q

what spp uses WARTHIN STARRY SILVER STAIN for diagnosis

a. haemophilus
b. bartonella
c. afia
d. legionella

A

Bartonella

diagnosis : biopsy (warthin starry silver stain)

B. henselae : primary causative agent of CAT SCRATCH DISEASE

17
Q

wihich of the following use reiterte’s stain

a. legionella
b. bartonella
c. bordetella
d. brucella

A

Legionella

isolated on enriched media : BCYE
stained by : REITERTE’S STAIN

18
Q

which of the following spp uses BIPHASIC CASTANEDA BOTTLE for culture media

a. legionella
b. bartonella
c. bordetella
d. brucella

A

brucella

19
Q

what is the screening test for early diagnosis of Legionella

a. culture
b. biopsy
c. urine ag test
d. motility test
e. string test

A

gold standard for identification of Legionella
- CULTURE (confirmatory)

Screening test for early diagnosis of Legionella
- urine antigen test (increase sensitivity)

20
Q

which spp produces shiny mercury droplets on Regan Lowe media

a. legionella pneumophila
b. bartonella henselae
c. bordetella pertussis
d. brucella

A

bordetella pertussis
culture media:
+ Bordet gengou
+ Regan Lowe - selective (SHINY MERCURY DROPLETS )

21
Q

which of the following spp can acquired through ingestion of contaminated animal products ( milk and meat)

a. legionella
b. bartonella
c. bordetella
d. brucella

A

brucella

  • human can acquired this spp by eating contaminated animal products such as meats and milk
  • agent of BRUCELLOSIS , UNDULANT FEVER AND MALTA FEVER
22
Q

agent of pontiac fever

a. legionella pneumophila
b. legionella micdadei
c. legionella bozemanni

A

Legionella pneumophila

  • agent of LEGIONNAIRE’S DISEASE (severe form)
  • agent of PONTIAC FEVER (milder form)
  • cooling towers for air conditioning and heating system
23
Q

wiga’s agent of pneumophila

a. legionella pneumophila
b. legionella micdadei
c. legionella bozemanni

A

legionella bozemanni
- wiga’s agent of pneumophila

legionella micdadei
- pittsburg pneumonia agent

24
Q

what stage of bordetella pertussis is bacterial multiplication

a. catarrhal
b. paroxysmal
c. convalescent

A

bordetella pertussis

three stages:
CATARRHAL - bacterial multiplication
PAROXYSMAL - violent and severe coughing
CONVALESCENT - last for 4 - 8 weeks

25
Q

which of the following spp of brucella is negative for thionin

a. b. abortus
b. b. melitensis
c. b. suis
d. b. canis

A

B. abortus
negative in thionin

positive in thionin

b. melitenss
b. suis
b. canis

26
Q

which of the following spp of brucella is positive for hydrogen peroxide

a. b. abortus
b. b. melitensis
c. b. suis
d. b. canis

A

HYDROGEN PEROXIDE
positive : B. abortus

negative

       b. melitenss
       b. suis
       b. canis
27
Q

implicated by animal bites /cat or dogs

a. brucella canis
b. pasteurella multocida
c. bordetella pertussis
d. francisella tularensis

A

Pasteurella multocida
- cat or dog bites

streptobacillus moniliformis
- rat bite fever / haverhill fever

28
Q

agent of Shipping fever in cattles

a. brucella canis
b. pasteurella multocida
c. bordetella pertussis
d. francisella tularensis

A

pasteurella multocida

  • etiologic agent of Pasteurellosis
  • agent of Shipping fever in cattles (hemorrhagic in humans)
29
Q

which of the ff spp has a musty or mushroom odor

a. brucella canis
b. pasteurella multocida
c. bordetella pertussis
d. francisella tularensis

A

pasteurella multocida

30
Q

which of the spp uses FORSHAYS SKIN TEST

a. brucella canis
b. pasteurella multocida
c. bordetella pertussis
d. francisella tularensis

A

francisella tularensis

tularemia skin test : forshays skin test
requires a special medium : BGCA

bacteriology.. (17 decks)

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