specimen collection, transport and processing

Question 1

which of the following is not component of stuart’s medium

a. methylene blue
b. thioglycolate
c. sodium glycerophosphate
d. calcium alginate

Answer 1

ans: d. calcium alginate
- also know as DACRON SWAB

components of stuart
a. methylene blue - redox indicator’

b. thioglycolate - reducing agent to permit survival of anaerobic bacteria
c. sodium glycerophosphate - to buffer calcium chloride

Question 2

what transport medium to be used in respiratory pathogen

a. stuart’s media
b. amie’s media
c. cary blair medium

Answer 2

ans: amie’s media

stuart’s is for bacteria and viral

cary blair is for stool (enteric pathogen)

Question 3

microscopic examination of clinical specimens

a. gram stain
b. acid fast stain
c. culture

Answer 3

gram stain

Question 4

what kind of media is both nutritive and differential

a. mac
b. bap
c. cap

Answer 4

bap

bap is based on hemolytic pattern
- allow the growth of gram negative bacteria

Question 5

this media is prepared by lysed red blood cell

a. mac
b. bap
c. cap

Answer 5

cap

the cell lysis provides the release of intracellular nutrients