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bacteriology.. > nonfermentative gram nega bacilli > Flashcards

nonfermentative gram nega bacilli Flashcards

1
Q

what medium used in oxidative fermentative test

a. bap
b. selenite f broth
c. hugh leifson medium
d. emb

A

hugh and leifson medium is an OF medium with 1% carbihydrate and bromthymol`

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2
Q

ph indicator of hugh leifson medium

a. phenol red
b. neutral red
c. bromthymol blue

A

bromthymol blue

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3
Q

1 cause of respiratory infection in cystic fibrosis patients

a. staphylococcus aureus
b. pseudomonas aeruginosa
c. pseudomonas putida

A

pseudomonas aeruginosa
- #1 cause of cystic fibrosis

staph aureus
- baterial pathogen causing respiratory among cystic fibrosis in <10 years old children

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4
Q

which of the following are valid testing for Pseudomonas aeruginosa

a. disk diffusion
b. agar dilution
c. broth dilution
d. neither a and b
d. aota

A

valid testing for Pseudomonas aeruginosa
(BAD)
B - BROTH DILUTION

A - AGAR DILUTION

D - DISK DIFFUSION

ans: aota

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5
Q

corn tortilla odor

a. pseudomonas aeruginosa
b. salmonella
c. klebsiella
d. burkholderia

A

pseudomonas aeruginosa

BAP : colonies have SPREADING FACTOR
METALLIC SHEEN
HAVE A BLE GREEN OR RED BROWN COLOR
GRAPE LIKE OR CORN TORTILLA ODOR

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6
Q

whch of the following organism acquired from contact lenses solution

a. P. aeruginosa
b. p. putida
c. p. fluorescens

A

p. aeruginosa

  • acquired through contaminated water or food, medical devices and through skin wound
  • contaminated contact lenses solution
  • also found on hot tubs and whirlpool baths
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7
Q

this organism is a causative agent of JACUZZI SYNDROME

a. P. aeruginosa
b. p. putida
c. p. fluorescens

A 
p. aeruginosa
-
causative agent  :  
   - ECTHYMA GANGRENOSUM
   - SWIMMER'S EAR
   - JACUZZI SYNDROME (NECROTIZING SKIN RASH)
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8
Q

which of the following organism is the etiologic agent of lung infection in cystic fibrosis patients

a. P. aeruginosa
b. p. putida
c. p. fluorescens

A

p. aeruginosa

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9
Q

what pigment only produced by P. aeruginosa

a. pyoverdin
b. pyocyanin
c. pyorubin
d. pyomelanin

A

ans: pyocyanin - blue pigment (only produced by p. aeruginosa)

pyoverdin- yellow green or yellow brown

pyorubin - red pigment

pyomelanin - brown or brown pigment

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10
Q

what species of pseudomonas can grow 42 degree celsius

a. P. aeruginosa
b. p. putida
c. p. fluorescens

A

p. aeruginosa - 42

p. putida and p. fluorescens - 4

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11
Q

what species of pseudomonas can grow on 4 degree celsius and have been linked to transfusion associated with septicemia

a. P. aeruginosa
b. p. putida
c. p. fluorescens

A

ans: B AND C

putida and fluorescens
- linked to transfusion associated with septicemia

pantoea agglomerans
- causes nosocomial outbreaks of septicemia DUE TO iv contamination

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12
Q

(+) gelatin hydrolysis

a. P. aeruginosa
b. p. putida
c. p. fluorescens

A

pseudomonas putida

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13
Q

can be isolated form cosmetics

a. P. aeruginosa
b. p. putida
c. p. fluorescens

A

pseudomonas putida and pseudomonas fluorescens

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14
Q

acetamide (+)

a. P. aeruginosa
b. p. putida
c. p. fluorescens

A

pseudomonas aeruginosa
acetamide (+)
can grow on cetrimide agar

putida nad fluorescens
acetamide (-)

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15
Q

what medium on pseudomonas promotes pigment production

a. sellers medium
b. bap
c. cetrimide agar
d. irigasan

A

sellers medium
- promotes pigment

cetrimide agar
- is a differential and selective medium that also enhances the pigment production (pyoverdin and pyocyanin) of p. aeruginosa

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16
Q

agent of glanders or farcy disease

a. pseudomonas aeruginosa
b. burkholderia mallei
c. burkholderia pseudomalaie
d. pseudomonas putida

A

burkholderia mallei

  • agent of GLADERS OR FARCY DISEASE
    • primary infectious disease on HORSES, DONKEYS, SHEEP AND GOATS
    • RESISTANT TO POLYMYXIN B
17
Q

whitmore’s bacillus

a. pseudomonas aeruginosa
b. burkholderia mallei
c. burkholderia pseudomalaie
d. pseudomonas putida
e. burkholderia cepacia

A

burkholderia pseudomallei

- whitmore’s bacillus

18
Q

it is isolated from ANESTHETIC , NEBULIZERS,DETERGENTS AND DISINFECTANTS

a. pseudomonas aeruginosa
b. burkholderia mallei
c. burkholderia cepacia
d. pseudomonas putida

A

BURKHOLDERIA CEPACIA

19
Q

the cause onion bulb rot and foot rot in humans

a. pseudomonas aeruginosa
b. burkholderia mallei
c. burkholderia pseudomalaie
d. burkholderia cepacia

A

burkholderia cepacia

20
Q

second most common cause of respiratory infection in cystic fibrosis

a. pseudomonas aeruginosa
b. burkholderia mallei
c. burkholderia pseudomalaie
d. burkholderia cepacia

A

ans. burkholderia cepacia

respiratory cystic fibrosis

  1. pseudomonas aeruginosa
  2. burkholderia cepacia
  3. s. aureus (<10 years old)
  4. h. influenzae
21
Q

which of the following is isolated from muddy soil

a. pseudomonas aeruginosa
b. burkholderia mallei
c. burkholderia pseudomalaie
d. pseudomonas putida

A

burkholderia pseudomallei

22
Q

what is the medium to used in burkholderia pseudomallei

a. ashdow medium with collistin
b. selenite f broth
c. bap
d. sellers medium

A

ans. ashdow medium with collistin

sellers medium is for pseudomonas aeruginosa

23
Q

EMB: cornflower blue

a. escherichia coli
b. burkholderia cepacia
c. acinetobacter baumanni
d. acinetobacter iwoffi

A

acinetobacter baumanni

EMB : cornflower blue
MAC : bluish pink or pink tint

24
Q

this gram negative bacilli mimic the appearance of neisseria in gram stain in female genital tract specimen

a. acinetobacter baumanni
b. acinetobacter baumanni
c. burkholderia mallei
d. pseudomonas putida

A

acinetobacter iwoffi

25
Q

gram negative bacilli that grows on 6.5%NaCl broth

a. acinetobacter
b. alcaligenes
c. burkholderia
d. pseudomonas

A

alcaligenes faecalis

26
Q

MAC : produce a feathered appearance. apha hemolytic and produces a fruity odor

a. acinetobacter
b. alcaligenes
c. burkholderia
d. pseudomonas

A

alcaligenes faecalis

MAC : feather edged
alpha hemolytic
fruit odor resemble apples or strawberries

bacteriology.. (17 decks)

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