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bacteriology.. > preliminary biochemical test > Flashcards

preliminary biochemical test Flashcards

1
Q

ph indicator for glucose fermentation test

a. methyl red
b. phenol red
c. neutral red

A

glucose fermentation: indicator - PHENOL RED

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2
Q

positive result for carbohydrate fermentation test

a. red color
b. yellow
c. violet

A

carbohydrate fermentation test

positive result : pink - red –> YELLOW

mannitol / CHO: carbohydrtae fermentation test
Mangga - yellow

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3
Q

enzymes which breaks down hydrogen peroxide into water and oxygen

a. catalase
b. coagulase
b. cytochrome oxidase

A

catalase

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4
Q

what is the reagent used in catalase test

a. 30% h2o2
b. 3% h2o2
c. trytophan

A

3% h2o2

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5
Q

positive result in catalase

a. purple/ violet
b. effervescence
c. clotting

A

catalase : cupious bubbling / effervescence

coagulase : clotting / clumping

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6
Q

positive result in microdase test

a. blue
b. purple
c. yellow

A

microdase test : blue

cytochrome oxidase : purple / violet

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7
Q

recommended used in oxidase test to transfer the growth

a. iron containing wire
b. wooden sticks
c. platinum wire

A

oxidase test: if an IRON containing wire is used to transfer growth, a FALSE POSITIVE REACTION may result; therefore, PLATINUM WIRE OR WOODEN STICKS ARE RECOMMENDED

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8
Q

an enzyme which converts fibrinogen to fibrin

a. coagulase
b. catalase
c. cytochrome oxidase

A

coagulase

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9
Q

what kind of bacteria produce bound and free coagulase

a. s. pyogenes
b. s. aureus
c. streptococcus

A

s. aureus

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10
Q

gold standard plasma used in coagulate test

a. rabbit’splasma
b. human plasma
c. lysed rbc

A

rabbit’s plasma

if not available rabbit’s plasma human plasma in EDTA can be used / alternate

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11
Q

tests to differentiate Enterococcus and streptococcus

a. coagulase
b. catalase
c. spot indole tests
d. pyr hydrolysis

A

pyr hydolysis

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12
Q

what type of hemolysis is incomplete hemolysis

a. alpha
b. beta
c. gamma
d. alpha prime

A

alpha

  • partial or incomplete lysis
  • greenish or brownish color
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13
Q

what type of hemolysis has a brownish color

a. alpha
b. beta
c. gamma
d. alpha prime

A

alpha

  • partial or incomplete lysis
  • greenish or brownish color
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14
Q

what type of hemolysis has a wide zone of beta hemolysis surrounding the small zone of alpha hemolysis

a. alpha prime
b. beta prime
c. gamma
d. zeta

A

alpha prime

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15
Q

positive result for indole test

a. purple ring
b. red pink ring
c. clotting

A

red pink ring

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16
Q

what kind of microscope is when the specimen image appears dark against a bright background

a. brightfield
b. darkfield
c. phase contrast

A

brightfield microscopy

17
Q

most critical step in performing gram stain procedure

a. primary stain
b. secondary stain
c. decolorizer
d. mordant

A

decolorizer

18
Q

what is the primary stain to be used in acid fast stain

a. crystal violet
b. carbol fuschin
c. malachitie green
d. torgitol

A

acid fast stain

primary - carbol fuschin
mordant - steam heat/ phenol / torgitol
decolorizer - acid alcohol
secondary stain - methylene blue or malachite green

19
Q

what to be decolorizer to be used in gram stain

a. acid alcohol
b. ethyl alcohol

A

gram stain - ethyl alcohol

acid fats stain - acid alcohol

20
Q

what method on acid fast stain is cold method

a. zielh neelsen method
b. kinyoun method

A

ziehl neelsen - hot

kinyoun - cold (kold)

21
Q

what is the positive control organism used in gram stain

a. staphylococcus aureus
b. staphylococcus pyogenes
c. e. coli

A

b & c
positive control : s. aureus and s. pyogenes

negative control : e. coli

22
Q

what is the negative control organism used in gram stain

a. staphylococcus aureus
b. staphylococcus pyogenes
c. e. coli

A

e .coli

23
Q

in afs what color is the result in secondary stain in non acid fast stain

a. blue
b. green
c. pink
d. red

A

if malachite green is used in secondary stain - green
while if methylene blue - blue

acid fast organism - red

bacteriology.. (17 decks)

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