Spectroscopy

Question 1

What is spectroscopy

Answer 1

Absorption of uv vis light making e go from ground to excited

Question 2

What do the different wavelengths show on spectroscopy

Answer 2

Types of bonds and transits of bonds

Question 3

What is beer lamberts law

Answer 3

A=ebc
A = ecl

Question 4

How is y=mx+c used

Answer 4

Determine conc of unknown from known solutions

Question 5

What is transmittance

Answer 5

Ratio of em radiation exiting from sample to source

Question 6

Define spectroscopy

Answer 6

Study of interaction of light with a substance to be analyser for its identification (qual) and amount (quant)

Absorption of em radiation + use of resulting absorption to study structural of organic molecules

Question 7

Describe alkanes and what theyre used for

Answer 7

Single bonds
Fuel

Question 8

Describe alkenes and what theyre used for

Answer 8

Double bond
Precursor to vitamin A

Question 9

Describe alkynes and what theyre used for

Answer 9

Triple bond
Calchiamicin anti tumour

Question 10

Describe alkyl halides and what theyre used for

Answer 10

4 functional groups with same properties
Mustard gas

Question 11

Describe alcohols and what theyre used for

Answer 11

OH
Hydroxyl
Carbohydrates often water soluble

Question 12

Describe ethers and what theyre used for

Answer 12

R-O-R

Question 13

Describe amines and what theyre used for

Answer 13

Amino acids
NH2

Question 14

Describe aldehydes and what theyre used for

Answer 14

H DOUBLE BOND O

Question 15

Describe ketones and what theyre used for

Answer 15

O double bond

Question 16

Describe carboxylic acid

Answer 16

COOH

Question 17

Describe esters

Answer 17

R. - COO - R

Question 18

Describe amides

Answer 18

C O NH2
Paracetamol

Question 19

Nitro group

Answer 19

NO2
Nitrazepam

Question 20

What components are used in UV/vis

Answer 20

Detector
Sample
Monochromator
Light

Question 21

What two lights are used in UV/Vis

Answer 21

Deuterium
Tungsten

Question 22

Describe spectroscopy

Answer 22

Absorption of uv/vis light by a molecule causing electron to go from ground to excited state

Question 23

What does T-100% mean

Answer 23

No absorption

Question 24

What does T-0% mean

Answer 24

Complete absorption

Question 25

What is the absolute definition of spectroscopy

Answer 25

Study of molecular structure & dynamics through absorption, emission + scattering of light

Question 26

What does low energy mean

Answer 26

High wavelength

Question 27

What is em radiation

Answer 27

Where both waves and particles called photons consist of regular electric and magnetic fluctuations

Question 28

What is beer lamberts law

Answer 28

A= ebc

Question 29

What is the relationship between absorbance and concentration

Answer 29

Directly proportional

Question 30

Limitation for beer lamberts law

Answer 30

Valid for low concs of analytes Only monochromatic validation allowed

Question 31

What does the wavelength absorption show

Answer 31

Transitions that occur by correlating to types of bonds formed

Question 32

What is the region for use of UV/vis

Answer 32

200-800nm

Question 33

What is a chromophore

Answer 33

Anything that absorbs light

Question 34

Why do we need to have a chromophore

Answer 34

To absorb light

Question 35

Why is line shape of spectra peaky

Answer 35

Molecules always vibrate and rotate

Question 36

Pharmaceutical applications of UV light

Answer 36

Quantification of drugs Find Pka values Dissolution testing Monitor reaction kinetics

Question 37

Absorption spectra

Answer 37

Bioanalytical method where signal intensity is directly proportional to conc

Question 38

Photoluminescence

Answer 38

Emissions following absorption of photon Return from high to low energy