Spectroscopy Flashcards
What is spectroscopy
Absorption of uv vis light making e go from ground to excited
What do the different wavelengths show on spectroscopy
Types of bonds and transits of bonds
What is beer lamberts law
A=ebc
A = ecl
How is y=mx+c used
Determine conc of unknown from known solutions
What is transmittance
Ratio of em radiation exiting from sample to source
Define spectroscopy
Study of interaction of light with a substance to be analyser for its identification (qual) and amount (quant)
Absorption of em radiation + use of resulting absorption to study structural of organic molecules
Describe alkanes and what theyre used for
Single bonds
Fuel
Describe alkenes and what theyre used for
Double bond
Precursor to vitamin A
Describe alkynes and what theyre used for
Triple bond
Calchiamicin anti tumour
Describe alkyl halides and what theyre used for
4 functional groups with same properties
Mustard gas
Describe alcohols and what theyre used for
OH
Hydroxyl
Carbohydrates often water soluble
Describe ethers and what theyre used for
R-O-R
Describe amines and what theyre used for
Amino acids
NH2
Describe aldehydes and what theyre used for
H DOUBLE BOND O
Describe ketones and what theyre used for
O double bond
Describe carboxylic acid
COOH
Describe esters
R. - COO - R
Describe amides
C O NH2
Paracetamol
Nitro group
NO2
Nitrazepam
What components are used in UV/vis
Detector
Sample
Monochromator
Light
What two lights are used in UV/Vis
Deuterium
Tungsten
Describe spectroscopy
Absorption of uv/vis light by a molecule causing electron to go from ground to excited state
What does T-100% mean
No absorption
What does T-0% mean
Complete absorption
What is the absolute definition of spectroscopy
Study of molecular structure & dynamics through absorption, emission + scattering of light
What does low energy mean
High wavelength
What is em radiation
Where both waves and particles called photons consist of regular electric and magnetic fluctuations
What is beer lamberts law
A= ebc
What is the relationship between absorbance and concentration
Directly proportional
Limitation for beer lamberts law
Valid for low concs of analytes
Only monochromatic validation allowed
What does the wavelength absorption show
Transitions that occur by correlating to types of bonds formed
What is the region for use of UV/vis
200-800nm
What is a chromophore
Anything that absorbs light
Why do we need to have a chromophore
To absorb light
Why is line shape of spectra peaky
Molecules always vibrate and rotate
Pharmaceutical applications of UV light
Quantification of drugs
Find Pka values
Dissolution testing
Monitor reaction kinetics
Absorption spectra
Bioanalytical method where signal intensity is directly proportional to conc
Photoluminescence
Emissions following absorption of photon
Return from high to low energy
