Specimen Collection

Question 0

How many swabs are preferred when requesting gram stain and culture

Answer 0

2 swabs

Question 1

Most common form of skin cleansing

Answer 1

70% alcohol followed by an iodophor

Question 2

Antiseptics to be used for specimen collection

Answer 2

Betadine

70% alcohol

Question 3

Inhibitor of bacterial growth

Answer 3

Fatty acids

Question 4

Why are CSF samples transported within 15 minutes

Answer 4

No preservatives

Question 5

Maximum hours when preserving CSF samples

Answer 5

Maximum 6 hours

Question 6

Pathogenic bacteria present in CSF

Answer 6

Neisseria meningitidis

Hemophilus influencing

Question 7

Maximum hours of transport

Answer 7

2 hours

Ideally: 30 minutes

Question 8

Preservation temp for g/s and c/s

Answer 8

35 C

Question 9

This preservative maintains the appropriate colony counts (urine)

Answer 9

Boric acid

Question 10

These are media that maintain the viability of microorganisms present in a specimen

Answer 10

Transport media

Question 11

Added to transport media to absorb fatty acids that could kill fastidious organisms

Answer 11

Charcoal

Question 12

Common stool transport medium

Answer 12

Buffered glycerol-saline

Question 13

Examples of transport media

Answer 13

Stuarts medium
Cary blair
Amies medium

Question 14

Used to prevent clotting of blood, bone marrow, synovial fluid

Answer 14

Anticoagulants

Question 15

Conc of sps used for blood culture

Answer 15

0.025% (w/v) sodium polyanethol sulfonate

Question 16

Loosen mucus of sputum

Answer 16

Digestion

Question 17

Specimen for afb

Answer 17

Sputum

Question 18

Needs decontamination

Answer 18

Sputum

Question 19

Top: stuart swab

Answer 19

Blue

Question 20

Top: aerobic swab

Answer 20

White

Question 21

Top: chlamydia swab

Answer 21

Yellow

Question 22

Top: cary blair

Answer 22

Red

Question 23

Top: mrsa pcr copan dual swab

Answer 23

Orange

Question 24

MRSA

Answer 24

Methycelene resistant s.aureus

Question 25

This type of stain helps visualize rods, cocci, wbc, rbs or squamous epithelial cells

Answer 25

Gram stain

Question 26

Acid fast stains

Answer 26

Ziehl-neelsen
Kinyoun
Auramine-rhodamine

Question 27

Fungal stains

Answer 27

Koh
Pas (periodic acid schiff)
Calcofluor white

Question 28

This process requires grinding of tissue

Answer 28

Homogenization

Question 29

Days required for culture media

Answer 29

3 days

Question 30

Days required for broth

Answer 30

7 days

Question 31

Form of oxigenation

Answer 31

Refrigenation

Question 32

Refrigerator temp

Answer 32

4 C

Question 33

Incubator temp

Answer 33

35 C

Question 34

Ambient temp

Answer 34

22 C

Question 35

Freezer temp

Answer 35

-20 to -70 C

Question 36

Swab collection that is collected by medtech

Answer 36

Throat swab collection

Question 37

PPM

Answer 37

Primary plated media

Question 38

Fluid in joints

Answer 38

Synovial fluid

Question 39

Fluid in the peritoneal of the abdomen

Answer 39

Peritoneal fluid

Question 40

Fluid in the lungs

Answer 40

Pleural fluid

Question 41

Automated blood culture system designed to do blood culture

Answer 41

Bacte alert

Question 42

Latest machine for blood culture

Answer 42

Bactec fx