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Microbiology > Specimen Collection > Flashcards

Specimen Collection Flashcards

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Biology 101 flashcards
0
Q

How many swabs are preferred when requesting gram stain and culture

A

2 swabs

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1
Q

Most common form of skin cleansing

A

70% alcohol followed by an iodophor

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2
Q

Antiseptics to be used for specimen collection

A

Betadine

70% alcohol

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3
Q

Inhibitor of bacterial growth

A

Fatty acids

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4
Q

Why are CSF samples transported within 15 minutes

A

No preservatives

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5
Q

Maximum hours when preserving CSF samples

A

Maximum 6 hours

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6
Q

Pathogenic bacteria present in CSF

A

Neisseria meningitidis

Hemophilus influencing

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7
Q

Maximum hours of transport

A

2 hours

Ideally: 30 minutes

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8
Q

Preservation temp for g/s and c/s

A

35 C

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9
Q

This preservative maintains the appropriate colony counts (urine)

A

Boric acid

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10
Q

These are media that maintain the viability of microorganisms present in a specimen

A

Transport media

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11
Q

Added to transport media to absorb fatty acids that could kill fastidious organisms

A

Charcoal

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12
Q

Common stool transport medium

A

Buffered glycerol-saline

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13
Q

Examples of transport media

A

Stuarts medium
Cary blair
Amies medium

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14
Q

Used to prevent clotting of blood, bone marrow, synovial fluid

A

Anticoagulants

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15
Q

Conc of sps used for blood culture

A

0.025% (w/v) sodium polyanethol sulfonate

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16
Q

Loosen mucus of sputum

A

Digestion

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17
Q

Specimen for afb

A

Sputum

18
Q

Needs decontamination

A

Sputum

19
Q

Top: stuart swab

A

Blue

20
Q

Top: aerobic swab

A

White

21
Q

Top: chlamydia swab

A

Yellow

22
Q

Top: cary blair

A

Red

23
Q

Top: mrsa pcr copan dual swab

A

Orange

24
Q

MRSA

A

Methycelene resistant s.aureus

25
Q

This type of stain helps visualize rods, cocci, wbc, rbs or squamous epithelial cells

A

Gram stain

26
Q

Acid fast stains

A

Ziehl-neelsen
Kinyoun
Auramine-rhodamine

27
Q

Fungal stains

A

Koh
Pas (periodic acid schiff)
Calcofluor white

28
Q

This process requires grinding of tissue

A

Homogenization

29
Q

Days required for culture media

A

3 days

30
Q

Days required for broth

A

7 days

31
Q

Form of oxigenation

A

Refrigenation

32
Q

Refrigerator temp

A

4 C

33
Q

Incubator temp

A

35 C

34
Q

Ambient temp

A

22 C

35
Q

Freezer temp

A

-20 to -70 C

36
Q

Swab collection that is collected by medtech

A

Throat swab collection

37
Q

PPM

A

Primary plated media

38
Q

Fluid in joints

A

Synovial fluid

39
Q

Fluid in the peritoneal of the abdomen

A

Peritoneal fluid

40
Q

Fluid in the lungs

A

Pleural fluid

41
Q

Automated blood culture system designed to do blood culture

A

Bacte alert

42
Q

Latest machine for blood culture

A

Bactec fx

Microbiology (10 decks)

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