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Gram Negative Cocci Flashcards

0
Q

Neiserria have an optimal growth in

A

Moist temp

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1
Q

Obligate aerobic
Non motile
Non hemolytic

A

Neisseria

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2
Q

How many percent of CO2 does neisseria have

A

2-8%

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3
Q

Carbohydrate fermenters of neiserria

A

Glucose

Maltose

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4
Q

Most of the colonies are pigmented exccept

A

N.flava
N.flavescens
N.subflava

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5
Q

Where is the Direct inoculation of specimens

A

At the bedside

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6
Q

Neiserria are sensitive to

A

Drying

Extremes of temp

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7
Q

Microscopy of neisseria

A

Gram - diplococci

With coffee or kidney bean shaped

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8
Q

Major pathogens in neiserria

A

N.gonorrheae

N.meningitidis

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9
Q

Biochemical tests in neiserria

A

+ oxidase

+ catalase

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10
Q

Never considered part of the normal flora

A

N.gonorrheae

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11
Q

Only known host in n.gonorrheae

A

Man

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12
Q

N.gonorrheae is found in

A

Urogenital tract
Anorectal area
Oropharynx
Conjunctiva

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13
Q

Mode of transmission of n.gonorrheae

A

Sexual contact

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14
Q

N.gonorrheae produce an

A

IgA protease

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15
Q

Antibody found in mucous membrane secretions

A

IgA

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16
Q

Culture in n.gonorrheae

A

CAP

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17
Q

Pvf of n.gonorrheae

A

Common pili

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18
Q

Colonial types

A

T1 & T2 (virulent)

T3 & T5 (avirulent)

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19
Q

Means of flow of seed and brothel

A

Gonorrheae

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20
Q

Incubation period of gonorrheae

A

2-7 days

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21
Q

Symptoms of gonorrheae

A

Purulent discharge
Lower abdominal pain
Dysuria (men)
Dysuria and vaginal bleeding (women)

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22
Q

Untreated gonococcal cervicitis may cause what

A

Sterility

Perihepatitis

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23
Q

It is a syndrome where untreated gonococcal cervicitis may cause sterility and perihepatitis

A

Fitz-hugh-curtis syndrome

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24
Q

Infection where there is rectal pain and bloody stools

A

Anorectal infections

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25
Q

Gonococcal eye infection during vaginal delivery through an infected birth canal

A

Conjunctivitis

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26
Q

Other term for conjunctivitis

A

Ophthalmia noenatorum

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27
Q

Most important factor in the lab diagnosis of n.gonorrhea

A

Specimen collection and handling

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28
Q

Specimens in n.gonorrheae

A 
Pus and secretions from urethra
Cervix
Prostate
Rectal mucosa
Throat
Joint fluid
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29
Q

Used for collection of the specimen

A

Dacron or rayon swabs

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30
Q

This swab should be avoided due to the presence of toxic fatty acids in the cotton fibers

A

Cotton swab

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31
Q

Non gonococcal urethritis

A

C.trachomatis

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32
Q

Smears for gs should be prepared from these specimens

A

Urogenital specimens

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33
Q

Cultures for confirmation

A

Bap

Cap

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34
Q

Specimens on swab should be prepared onto media in what pattern

A

Z pattern
And
Cross streaked with a loop

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35
Q

Agar with all the TMA components + trimethoprim lactate

A

Modified thayer martin agar

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36
Q

Agar where vancomycin conc. is increased

A

Martin lewis medium

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37
Q

Chocolate agar with an enrichment supplement and antibiotics

A

Thayer martin agar

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38
Q

A transparent medium with lysed horse blood, horse plasma, and yeast dialysate

A

Nyc medium

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39
Q

Antimicrobial agents in nyc medium

A

Vancomycin
Colistin
Trimethoprim
Amphotericin B

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40
Q

Genital mycoplasma

A

Mycoplasma hominis

Ureaplasma urealyticum

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41
Q

Examples of transport medium

A

Cary blair
Amies
Jembec
Transgrow

42
Q

Required temp for incubation of agar plates

A

35-37 degree celcius

43
Q

Incubation period for agar plates

44
Q

Standard method of identifying n.gonorrheae

A

CHO utilization test

45
Q

Medium used in cho urilization test

A

CTA (cystine trypticase agar)

46
Q

Composition of cta

A

1% carbohydrate

Phenol red

47
Q

+ result in cho utilization test

A

Yellow color

within 24-72 hrs at 35 degree celcius

48
Q

Rgt in oxidase test

A

1% tetramethyl-p-phenylenediamine-dihydrochloride

49
Q

+ result in oxidase test

A

Purple color within 10 seconds

50
Q

Other + oxidase bacteria

A

N.cinerea
N.meningitidis
M.catarrhalis

51
Q

Rgt used in superoxol test

A

20-30% H2O2

52
Q

+ result in superoxol test

A

Vigourous bubbling

53
Q

+ in dnase test

- in dnase test

A

M.catarrhalis +

N.gonorrheae -

54
Q

Highly specific and sensitive test

A

Fat
Or
Fluorescent antibody test

55
Q

Test that confirms the morphologic appearance of the bacteria

56
Q

Confirms the biochemicql identification of n.gonorrheae

A

Coagglutination

57
Q

+ result of coagglutination

A

Agglutination

58
Q

Nucleic acid amplification

A

Molecular assays

59
Q

Specimen for molecular assay

A

Urethral swabs

60
Q

Blood protein

A

Complement

61
Q

Used for culture confirmation or direct detection in endocervical or urethral swab specimens

A

Chemiluminescent nucleic acid probe

62
Q

Causative agent of epidemic meningococcal meningitis/meningococcemia/cerebrospinal fever/spotted fever

A

N.meningitidis

63
Q

N.meningitidis is the leading cause of

A

Fatal bacterial meningitis

64
Q

May be found as a commensal inhabitant of the upper respiratory tract of the carriers

A

N.meningitis

65
Q

What kind of fermenters are n.meningitidis

A

Glucose and maltose fermenters

66
Q

Microscopy of n.meningitidis

A

Encapsulated strains with halo around organism

67
Q

Serogroups of n.meningitidis

A 
A
B
C
Y
W-135
68
Q

Virulence factors of n.meningitidis

A

Pili
Polysaccharide capsule
IgA1

69
Q

Coverings of the brain

70
Q

Refers to the presence of n.meningitidis in the blood

A

Meningococcemia

71
Q

Source of epidemics in meningococcemia

A

Oral secretions

Respiratory droplets

72
Q

Signs and symptoms of meningococcemia

A

Frontal headache
Stiff neck
Fever

73
Q

They are develop because of endotoxins

A

Petechial skin lesions

74
Q

Syndrome where there is production of hemorrhage in the adrenals

A

Waterhouse-friderichsen syndrome

75
Q

Specimen in n.meningitidis

A

Csf
Blood
Nasopharyngeal swab
Petechial skin lesions

76
Q

N. Meningitidis is senstive to

77
Q

Process of concentarting csf fluid

A

Cytocentrifugation

78
Q

When is the highest yield of positive of csf gram stain is obtained

A

When specimens are concentrated

79
Q

Culture media in n.meningitidis

80
Q

This resembles n.meningitidis on gonococcal selective media

A

N.lactamica

81
Q

Orher term for beta lactamase test

A

Cephalosporinase test

82
Q

Rgt used in beta lactamase test

A

Cephalosporin disk

83
Q

+ result in beta lactamase test

A

Deep pink
Or
Red color

84
Q

Other name of moraxella catarrhalis

A

Branhamella catarrhalis

85
Q

How does m.catarrhalis resembles neiserria

A

By exhibiting gram - coccal morphology

86
Q

Mos commonly isolatmemberof the genus moraxella

A

M.catarrhalis

87
Q

Location of m.catarrhalis

A

Oropharynx

88
Q

M.catrrahlis are only isolated from

89
Q

3rd most common cause of otitis media and sinusitis in children

A

M.catarrhalis

90
Q

Culture in m.catarrhalis

A

Honeypuck appearance

91
Q

Biochemical tests in m.catarrhalis

A

+ oxidase

+ catalase

92
Q

Does not ferment any sugar

A

Asaccharolytic

93
Q

Cause of otitis media

A

M.catarrhalis

94
Q

Specimen in butyrate esterase test

A

Ear culture

95
Q

+ result in butyrate esterase test in m.catarrhalis

A

Blue color

96
Q

Protein in blood

A

Transferrin

97
Q

Organ of attachment

A

Common pili

98
Q

Complication of PID

A

Fitz hugh curtis syndrome

99
Q

Prevent swarming

A

Trimethoprim lactate

100
Q

Inhibits gram -

101
Q

Inhibits gram +

A

Vancomycin

102
Q

Screening test

A

Molecular assay

Microbiology (10 decks)

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