Gram + Bacilli (acid Fast Bacilli) Flashcards
0
Q
Required time for 2 divisions
A
20 mins
1
Q
Inclusion bodies
A
Much’s granules
2
Q
Distinct X, Y, V
A
Corynebacterium
3
Q
Cell wall of mycobacteria contains
A
N-glycolylmuramic acid
4
Q
What will you call if the cell wall resists decolorization with acid-ethanol
A
Acid fastness
5
Q
Beaded appearance means
A
Incomplete staining
6
Q
Appearance of mycobacteria
A
Gram neutral or gram ghosts appearance
7
Q
Ph requirement of mycobacteria
A
6.5-6.8
8
Q
2 groups of mycobacteria
A
Mycobacterium tuberculosis complex (mtc) Nontuberculosis mycobacteria (ntm)
9
Q
Species causing tuberculosis
A
Mycobacterium tuberculosis complex
10
Q
Species that do not cause tuberculosis
A
Nontuberculosis mycobacteria
11
Q
Other term for mycobacterium tuberculosis
A
Koch bacillus
12
Q
Most popular mtc
A
Koch bacillus
13
Q
Replication time of m.tuberculosis
A
20-22 hrs
14
Q
M.tuberculosis is inhibited by
A
nitroimidazopyran (nap)
15
Q
Cauliflower colonies
A
M.tuberculosis
16
Q
Curved strands of bacilli
A
Cording
17
Q
+ result of T2H
A
Without growth
18
Q
Virulence factor of m.tuberculosis
A
Cord factor
19
Q
Produces tb in animals
A
Mycobacterium bovis
20
Q
M.bovis attenuated strain is used for
A
Vaccination (BCG/ Bacille-Calmette-Guerin)
21
Q
Mot of m.bovis
A
By ingestion of contaminated milk from infected cows or by exposure to infected animals
22
Q
- niacin
A
M.bovis
23
Q
+ niacin
A
M.tuberculosis
24
Is the smooth strain of m.tuberculosis
M.canettii
25
Disease of the respiratory tract
Tuberculosis
26
Tuberculosis is also known as
Consumption
27
Contribute greatly to the progression to active tb
Homelessness
Alcoholism
Incarceration
28
Usually limited to detection of a positive tuberculin test using purified protein derivative
Primary tuberculosis
29
Mtb cells are phagocytized by
Alveolar macrophages
30
This strain develops when only one antimycobacterial gent is used
Mdr-tb
| Multidrug-resistant mycobacterium tuberculosis
31
species of photochromogens
M.kansasii
M.asiaticum
M.marinum
M.simiae
32
Used for staining fungi
PAS
33
Aka anonymous, atypical, unclassified, unknown, tuberculoid, MOTT
Nontuberculosis mycobacteria (ntm)
34
Slow growers
Ntm
35
Important pathogens in immunocompromised and immunocompotent populations
M.avium complex (mac)
36
Biochemical tests for m.avium complex
+ heat stable catalase
37
Wood pigeon bacillus
M.avium subsp. silvaticum
38
Cause of disease in poultry and swine
M.avium
39
Causative agent of johne's disease
M.avium subsp. paratuberculosis
40
It is isolated from bowel mucosa of patients with crohn's disease
M.avium subsp. paratuberculosis
41
Growth factor of m.avium subsp paratuberculosis
Mycobactin
42
Yellow bacillus
M.kansasii
43
Second to mac causing ntm lung disease
M.kansasii
44
Shepherd's crook or candy cane
M.kansasii
45
Rapid hydrolyzer
+ tween 80 hydrolysis
46
Swimming pool granuloma
M.marinum
47
3rd most common mycobacterium species
M.ulcerans
48
Rare cause of buruli ulcer
M.ulcerans
49
Tap water bacillus
M.gordonae
50
Problem in the preparation of bacteriologic smears
M.tuberculosis
51
Recovered from hot and cold water taps
M.xenopi
52
Birds nest appearance with sticlike projections
M.xenopi
53
Radish bacillus
M.terrae-complex
54
Weakly gram positive rods resembling diphtheroids
Nontuberculosis mycobacteria-rapid growers
55
Most common isolate in nontuberculosis mycobacteria-rapid growers
M.fortuitum
56
Most common rapidly growing mycobacteria associated with localized cutaneous infections
M.fortuitum
57
Procedures are done in what type of BSC
Class 2 or 3
58
Preferred specimen for sputum
Early morning sputum (3 consecutive days)
59
Preferred specimen for urine
First morning midstream urine
60
This sputum is unaacceptable because of inc. contamination
Pooled sputa
61
This collection is done on ambulatory patients who are unable to follow instructions
Aerosol induced
62
Used to recover mycobacteria that may have been swallowed during the night
Gastric lavage
63
Required volume for sputum
5-10 ml
64
Required volume for gastric lavage
20-25 ml of gastric secretions
65
How many specimens are being collected in gastric lavage
3
66
It is the specimen of choice for detecting NTM
Bronchoscopy specimens
67
This specimens are used to odentify patients who may be at risk of developing M.avium-complex disease
Fecal specimens
68
Collected to diagnose individuals with AIDS
Blood
69
Culture medium for blood
BACTEC 13A vial
70
Grading of sputum specimens
Bartlett's criteria
Murray-washington method
Heineman's method
71
This method emphasizes the ratio between squamous epithelial cells and polymorphonuclear cells
Heineman's method
72
This criteria is for scoring sputum
Bartletts criteria
73
Dissolves mucus so that the bacteria can use nutrients in the medium
Digestion
74
For removing all bacteria except mycobacteria
Decontamination
75
Reagent used for decontamination of sputum specimens
5%-6% oxalic acid
76
Most common decontamination agent
| Serves as both decontaminant and digestant
2-4% NaOH
77
Ideal for transport of specimen
1% cetyl-pyridium chloride
78
Also known as dithiothreitol; a digestion agent
NALC (N-acetyl-L-cysteine)
79
Distinct acid fast organisms
Mycobaterium
Cryptosporidium
Isospora
80
Parasites not bacteria
Cryptosporidium
| Isospora
81
Partially acid fast organisms
Legionella micdadei
Rhodococcus
Nocardia
82
Afb are examined at
250x and 400x maginification
83
Auramine-tained smears may be restrained by these methods
Ziel-neelsen
| Kinyoun
84
This method is sensitive, reliable and specific
Auramine-rhodamine fluorochrome
85
+ result of auramine-rhodamine fluorochrome
Bright yellow-orange bacilli against a dark background
86
Days for blood culture
5-7
87
Days for bap and cap
3
88
This media is incubated at a slanted position to
Tubed media
89
Inhibitory agent of nonmycobacteria
Malachite green
90
Grow best at 42 degree celcius
M.xenopi
91
Commonly used egg based medium
Lowenstein jensen
92
Medium used for recovery of mycobacteria
Petragnani medium
93
Medium used for m.avium complex
Wallenstein medium
94
Other term for transparent media
Serum agar based media
95
This results to release of formaldehyde
Excessive heating
| Light exposure
96
Contains 0.1% casein hydrolysate
Middlebrook 7H10-11
97
Most commonly used liquid/broth media
| Improves the isolation rate of mycobacteria
Bactec 12B
| Bactec 13B
98
Nonselective medium used for subculturing stock strains
Middlebrook 7H9 broth
99
Biphasic media
| For rapid growth and identification of mycobacteria
Septi-check AFB
100
MGIT 960 system results
42 days
101
Presence of mycobacteria leads to
Comsumption of oxygen resulting to release of CO2
102
Depletion of free oxygen resulting to
Fluorescence
103
Virulence test for infants
Vollmer patch test
104
Scratching method
Von-pirquet
105
Puncture test
Tine test
106
Reagent used in heat stable catalase test
30% H2O2
107
+ result in heat stable catalase test
Column of bubbles
108
High level heat stable catalase positive
M.asiaticum
M.terrae complex
M.kansasii
M.simiae
109
Heat stable catalase negative
Mtb complex
| M.marinum
110
Commonly used biochemical test for the identification of mtb and other slow growing mycobacteria
Niacin test
111
Rgt used in niacin tests
Cyanogen bromide
+
Aniline rgt
112
+ result in niacin test
Yellow color
113
Indicator rgt used in nitrate reduction test
Zinc
114
Other term for nitrate reduction test
Broth method
115
+ result in nitrate reduction test
Red color
116
Rgts used in nitrate reduction test
Sulfanamide + N-napthylenediamine dihydrochloride + HCL
117
this enzyme breaks down phenolphthalein disulfate into free phenolphthalein
Arylsulfatase
118
Rgts used in arylsulfatase test
Potassium phenolphthalein SO4 + sodium bicarbonate
119
+ 3 day and 14 day arylsulfatase test
M.fortuitum
M.chelonae
M.xenopi
120
Useful for separating species of nonphotochromogens and scotochromogens
Tween 80 hydrolysis
121
With or without, there are colored colonies
Scotochromogens
122
Substrate in tween 80 hydrolysis
Oleic acid
123
+ result in tween 80 hydrolysis
Pink color - m.kansasii
124
Non cultivatable NTM
M.leprae
125
Other term for m.leprae
Hansens bacillus
126
Microscopy: m.leprae
Cigar pocket
| Pocket fence
127
Other term for hansens disease
Leprosy
128
Lepros means
Scaly
Scabby
Rough
129
Mot for leprosy
Person to person through inhalation
