Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology > Gram + Bacilli (acid Fast Bacilli) > Flashcards

Gram + Bacilli (acid Fast Bacilli) Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
0
Q

Required time for 2 divisions

A

20 mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
1
Q

Inclusion bodies

A

Much’s granules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Distinct X, Y, V

A

Corynebacterium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Cell wall of mycobacteria contains

A

N-glycolylmuramic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What will you call if the cell wall resists decolorization with acid-ethanol

A

Acid fastness

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Beaded appearance means

A

Incomplete staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Appearance of mycobacteria

A

Gram neutral or gram ghosts appearance

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Ph requirement of mycobacteria

A

6.5-6.8

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

2 groups of mycobacteria

A 
Mycobacterium tuberculosis complex (mtc)
Nontuberculosis mycobacteria (ntm)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Species causing tuberculosis

A

Mycobacterium tuberculosis complex

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Species that do not cause tuberculosis

A

Nontuberculosis mycobacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Other term for mycobacterium tuberculosis

A

Koch bacillus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Most popular mtc

A

Koch bacillus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Replication time of m.tuberculosis

A

20-22 hrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

M.tuberculosis is inhibited by

A

nitroimidazopyran (nap)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Cauliflower colonies

A

M.tuberculosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Curved strands of bacilli

A

Cording

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

+ result of T2H

A

Without growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Virulence factor of m.tuberculosis

A

Cord factor

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Produces tb in animals

A

Mycobacterium bovis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

M.bovis attenuated strain is used for

A

Vaccination (BCG/ Bacille-Calmette-Guerin)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Mot of m.bovis

A

By ingestion of contaminated milk from infected cows or by exposure to infected animals

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q
  • niacin
A

M.bovis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

+ niacin

A

M.tuberculosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Is the smooth strain of m.tuberculosis

A

M.canettii

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Disease of the respiratory tract

A

Tuberculosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Tuberculosis is also known as

A

Consumption

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Contribute greatly to the progression to active tb

A

Homelessness
Alcoholism
Incarceration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

Usually limited to detection of a positive tuberculin test using purified protein derivative

A

Primary tuberculosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

Mtb cells are phagocytized by

A

Alveolar macrophages

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

This strain develops when only one antimycobacterial gent is used

A

Mdr-tb

Multidrug-resistant mycobacterium tuberculosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

species of photochromogens

A

M.kansasii
M.asiaticum
M.marinum
M.simiae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

Used for staining fungi

A

PAS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

Aka anonymous, atypical, unclassified, unknown, tuberculoid, MOTT

A

Nontuberculosis mycobacteria (ntm)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

Slow growers

A

Ntm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

Important pathogens in immunocompromised and immunocompotent populations

A

M.avium complex (mac)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

Biochemical tests for m.avium complex

A

+ heat stable catalase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

Wood pigeon bacillus

A

M.avium subsp. silvaticum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

Cause of disease in poultry and swine

A

M.avium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

Causative agent of johne’s disease

A

M.avium subsp. paratuberculosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
40
Q

It is isolated from bowel mucosa of patients with crohn’s disease

A

M.avium subsp. paratuberculosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
41
Q

Growth factor of m.avium subsp paratuberculosis

A

Mycobactin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
42
Q

Yellow bacillus

A

M.kansasii

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
43
Q

Second to mac causing ntm lung disease

A

M.kansasii

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
44
Q

Shepherd’s crook or candy cane

A

M.kansasii

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
45
Q

Rapid hydrolyzer

A

+ tween 80 hydrolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
46
Q

Swimming pool granuloma

A

M.marinum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
47
Q

3rd most common mycobacterium species

A

M.ulcerans

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
48
Q

Rare cause of buruli ulcer

A

M.ulcerans

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
49
Q

Tap water bacillus

A

M.gordonae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
50
Q

Problem in the preparation of bacteriologic smears

A

M.tuberculosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
51
Q

Recovered from hot and cold water taps

A

M.xenopi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
52
Q

Birds nest appearance with sticlike projections

A

M.xenopi

53
Q

Radish bacillus

A

M.terrae-complex

54
Q

Weakly gram positive rods resembling diphtheroids

A

Nontuberculosis mycobacteria-rapid growers

55
Q

Most common isolate in nontuberculosis mycobacteria-rapid growers

A

M.fortuitum

56
Q

Most common rapidly growing mycobacteria associated with localized cutaneous infections

A

M.fortuitum

57
Q

Procedures are done in what type of BSC

A

Class 2 or 3

58
Q

Preferred specimen for sputum

A

Early morning sputum (3 consecutive days)

59
Q

Preferred specimen for urine

A

First morning midstream urine

60
Q

This sputum is unaacceptable because of inc. contamination

A

Pooled sputa

61
Q

This collection is done on ambulatory patients who are unable to follow instructions

A

Aerosol induced

62
Q

Used to recover mycobacteria that may have been swallowed during the night

A

Gastric lavage

63
Q

Required volume for sputum

A

5-10 ml

64
Q

Required volume for gastric lavage

A

20-25 ml of gastric secretions

65
Q

How many specimens are being collected in gastric lavage

A

3

66
Q

It is the specimen of choice for detecting NTM

A

Bronchoscopy specimens

67
Q

This specimens are used to odentify patients who may be at risk of developing M.avium-complex disease

A

Fecal specimens

68
Q

Collected to diagnose individuals with AIDS

A

Blood

69
Q

Culture medium for blood

A

BACTEC 13A vial

70
Q

Grading of sputum specimens

A

Bartlett’s criteria
Murray-washington method
Heineman’s method

71
Q

This method emphasizes the ratio between squamous epithelial cells and polymorphonuclear cells

A

Heineman’s method

72
Q

This criteria is for scoring sputum

A

Bartletts criteria

73
Q

Dissolves mucus so that the bacteria can use nutrients in the medium

A

Digestion

74
Q

For removing all bacteria except mycobacteria

A

Decontamination

75
Q

Reagent used for decontamination of sputum specimens

A

5%-6% oxalic acid

76
Q

Most common decontamination agent

Serves as both decontaminant and digestant

A

2-4% NaOH

77
Q

Ideal for transport of specimen

A

1% cetyl-pyridium chloride

78
Q

Also known as dithiothreitol; a digestion agent

A

NALC (N-acetyl-L-cysteine)

79
Q

Distinct acid fast organisms

A

Mycobaterium
Cryptosporidium
Isospora

80
Q

Parasites not bacteria

A

Cryptosporidium

Isospora

81
Q

Partially acid fast organisms

A

Legionella micdadei
Rhodococcus
Nocardia

82
Q

Afb are examined at

A

250x and 400x maginification

83
Q

Auramine-tained smears may be restrained by these methods

A

Ziel-neelsen

Kinyoun

84
Q

This method is sensitive, reliable and specific

A

Auramine-rhodamine fluorochrome

85
Q

+ result of auramine-rhodamine fluorochrome

A

Bright yellow-orange bacilli against a dark background

86
Q

Days for blood culture

A

5-7

87
Q

Days for bap and cap

A

3

88
Q

This media is incubated at a slanted position to

A

Tubed media

89
Q

Inhibitory agent of nonmycobacteria

A

Malachite green

90
Q

Grow best at 42 degree celcius

A

M.xenopi

91
Q

Commonly used egg based medium

A

Lowenstein jensen

92
Q

Medium used for recovery of mycobacteria

A

Petragnani medium

93
Q

Medium used for m.avium complex

A

Wallenstein medium

94
Q

Other term for transparent media

A

Serum agar based media

95
Q

This results to release of formaldehyde

A

Excessive heating

Light exposure

96
Q

Contains 0.1% casein hydrolysate

A

Middlebrook 7H10-11

97
Q

Most commonly used liquid/broth media

Improves the isolation rate of mycobacteria

A

Bactec 12B

Bactec 13B

98
Q

Nonselective medium used for subculturing stock strains

A

Middlebrook 7H9 broth

99
Q

Biphasic media

For rapid growth and identification of mycobacteria

A

Septi-check AFB

100
Q

MGIT 960 system results

A

42 days

101
Q

Presence of mycobacteria leads to

A

Comsumption of oxygen resulting to release of CO2

102
Q

Depletion of free oxygen resulting to

A

Fluorescence

103
Q

Virulence test for infants

A

Vollmer patch test

104
Q

Scratching method

A

Von-pirquet

105
Q

Puncture test

A

Tine test

106
Q

Reagent used in heat stable catalase test

A

30% H2O2

107
Q

+ result in heat stable catalase test

A

Column of bubbles

108
Q

High level heat stable catalase positive

A

M.asiaticum
M.terrae complex
M.kansasii
M.simiae

109
Q

Heat stable catalase negative

A

Mtb complex

M.marinum

110
Q

Commonly used biochemical test for the identification of mtb and other slow growing mycobacteria

A

Niacin test

111
Q

Rgt used in niacin tests

A

Cyanogen bromide
+
Aniline rgt

112
Q

+ result in niacin test

A

Yellow color

113
Q

Indicator rgt used in nitrate reduction test

A

Zinc

114
Q

Other term for nitrate reduction test

A

Broth method

115
Q

+ result in nitrate reduction test

A

Red color

116
Q

Rgts used in nitrate reduction test

A

Sulfanamide + N-napthylenediamine dihydrochloride + HCL

117
Q

this enzyme breaks down phenolphthalein disulfate into free phenolphthalein

A

Arylsulfatase

118
Q

Rgts used in arylsulfatase test

A

Potassium phenolphthalein SO4 + sodium bicarbonate

119
Q

+ 3 day and 14 day arylsulfatase test

A

M.fortuitum
M.chelonae
M.xenopi

120
Q

Useful for separating species of nonphotochromogens and scotochromogens

A

Tween 80 hydrolysis

121
Q

With or without, there are colored colonies

A

Scotochromogens

122
Q

Substrate in tween 80 hydrolysis

A

Oleic acid

123
Q

+ result in tween 80 hydrolysis

A

Pink color - m.kansasii

124
Q

Non cultivatable NTM

A

M.leprae

125
Q

Other term for m.leprae

A

Hansens bacillus

126
Q

Microscopy: m.leprae

A

Cigar pocket

Pocket fence

127
Q

Other term for hansens disease

A

Leprosy

128
Q

Lepros means

A

Scaly
Scabby
Rough

129
Q

Mot for leprosy

A

Person to person through inhalation

Microbiology (10 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions