Antimicrobial Testing Flashcards
Standard inoculum size for broth dilution method
5 x 10 ^ 5 CFU/ml
Susceptibility medium in broth dilution method
Mueller hinton broth
Reagent in broth dilution method
2% NaCl
The lowest conc of the antimicrobial agent which inhibits the bacterial growth
MIC
Minimum inhibitory conc
The lowest conc of the antimicrobial agent needed to kill the bacterial growth
MBC
minimum bactericidal conc
The lowest conc of the antimicrobial agent which kills the bacterial growth
MLC
Minimum lethal conc
Reference method for testing anaerobes and n.gonorrhreae
Agar dilution method
Media for anaerobes
Brucella laked sheep blood
Method for brucella laked sheep blood
Wadsworth method
Standard inoculum size in agar dilution method
1 x 10 ^ 4 CFU/ml
Other name of disk diffusion method
Kirby bauer test
Most commonly used AST
Kirby bauer test
Standard inoculum size for disk diffusion method
1.5 x 10^ 8 CFU/ml
Standard size of disks
6 mm
This suspension is preferred for fastidious bacteria
Direct inoculum suspension
Zone of inhibition
17 mm
Ideal age of cultures
18-24 hours old
Smaller zones means
False resistance
Larger zones means
False susceptiblity
Prolonged incubation and diminish activity means
False resistance
Ph of the medium for zone of inhibition
7.2-7.4
A dilution test based on the diffusion of a continuous conc gradient of an antimicrobial agent
E test (epsilometer)
+ result in e test
Ellipse of growth inhibition
This test is where 2 antibiotics help kill the bacteria
Synergy test
Other name for serum bactericidal test
Schlicter test
Most commonly used machine for automated antimicrobial susceptibility test method
Vitek system
Device used for growth detection
Flurometer
