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Microbiology > Antimicrobial Testing > Flashcards

Antimicrobial Testing Flashcards

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Biology 101 flashcards
0
Q

Standard inoculum size for broth dilution method

A

5 x 10 ^ 5 CFU/ml

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1
Q

Susceptibility medium in broth dilution method

A

Mueller hinton broth

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2
Q

Reagent in broth dilution method

A

2% NaCl

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3
Q

The lowest conc of the antimicrobial agent which inhibits the bacterial growth

A

MIC

Minimum inhibitory conc

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4
Q

The lowest conc of the antimicrobial agent needed to kill the bacterial growth

A

MBC

minimum bactericidal conc

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5
Q

The lowest conc of the antimicrobial agent which kills the bacterial growth

A

MLC

Minimum lethal conc

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6
Q

Reference method for testing anaerobes and n.gonorrhreae

A

Agar dilution method

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7
Q

Media for anaerobes

A

Brucella laked sheep blood

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8
Q

Method for brucella laked sheep blood

A

Wadsworth method

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9
Q

Standard inoculum size in agar dilution method

A

1 x 10 ^ 4 CFU/ml

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10
Q

Other name of disk diffusion method

A

Kirby bauer test

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11
Q

Most commonly used AST

A

Kirby bauer test

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12
Q

Standard inoculum size for disk diffusion method

A

1.5 x 10^ 8 CFU/ml

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13
Q

Standard size of disks

A

6 mm

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14
Q

This suspension is preferred for fastidious bacteria

A

Direct inoculum suspension

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15
Q

Zone of inhibition

A

17 mm

16
Q

Ideal age of cultures

A

18-24 hours old

17
Q

Smaller zones means

A

False resistance

18
Q

Larger zones means

A

False susceptiblity

19
Q

Prolonged incubation and diminish activity means

A

False resistance

20
Q

Ph of the medium for zone of inhibition

A

7.2-7.4

21
Q

A dilution test based on the diffusion of a continuous conc gradient of an antimicrobial agent

A

E test (epsilometer)

22
Q

+ result in e test

A

Ellipse of growth inhibition

23
Q

This test is where 2 antibiotics help kill the bacteria

A

Synergy test

24
Q

Other name for serum bactericidal test

A

Schlicter test

25
Q

Most commonly used machine for automated antimicrobial susceptibility test method

A

Vitek system

26
Q

Device used for growth detection

A

Flurometer

Microbiology (10 decks)

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