SLIDES: Visualizing cells (ch 9)

Question 1

How much can you see with normal light microscopy?

Answer 1

Up to cells

Question 2

What can fluorescent microscopy do?

Answer 2

Make specific dyed molecules light up, lets you see where groups of them are

Question 3

How is confocal microscopy useful?

Answer 3

You can get higher resolution by excluding light you don’t need with a laser

Question 4

How is a transmission electron microscope (TEM) similar to a light microscope?

Answer 4

They both have lenses, just in different forms. EM’s use magnetic coils instead of glass and electrons instead of light

Question 5

Which electron microscope directly makes a 3D image?

Answer 5

Scanning electron microscope (SEM)

Question 6

Around how small can the best electron microscopes see?

Answer 6

~2nm or ~.002um or ~.000002mm

Question 7

Around how small can the best electron microscopes see?

Answer 7

~2nm or ~.002um or ~.000002mm

Question 8

What are the types of light microscopes?

Answer 8

Normal, fluorescent (light up molecules), confocal (exclude light you don’t need)

Question 9

Does a transmission electron microscope (TEM) or scanning electron microscope (SEM) have better resolution?

Answer 9

SEM, it gets gorgeous 3D images