Simmons Citrate Test Pg95-96 Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

The ability of some bacteria to utilize citrate as a sole carbon source is tested for using

A

Simmons citrate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Simmons citrate agar contains _______ as the sole carbon source, ammonium salt instead of amino acids as the sole nitrogen source and a pH indicator:____________

A

-contains citrate
- bromythmol blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Bromythmol blue is what color at neutral pH and turns what color above pH 7.6

A

Green at neutral pH
Turns blue above pH 7.6

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

In a positive citrate test, heavy growth generally turns the bromythmol blue indicator in the medium to a….. (what color?)

A

Deep Prussian blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

This occurs because growing bacteria produce alkaline products from the _________ in the medium

A

Ammonium salts

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What type of growth occurs in a negative reaction and what is the color of the agar?

A

There is little to no growth and the color of the agar remains unchanged (green)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Sodium citrate equation

A

Citratase
Sodium citrate (Na C6H8O7)——————> Acetate (C2H4O2)+ Oxalacetate (C4H4O5)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Procedure: what bacteria is being used with the Simmons citrate agar slants?

A

E. coli and E. aerogenes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Procedure: how long are cultures incubated for at 37 degrees Celsius?

A

48 hours

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Results:

A

Medium: Simmons citrate agar
Substrate: citrate
Reagents post-incubation: bromythmol blue
+ End prods: acetate and oxalacetate
+ appearance: blue
- appearance: green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Was the citrate utilized for E.coli?

A

Citrate was NOT Utilized.
(No heavy growth- remained dark green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Was the citrate utilized for E.aerogenes?

A

Yes, heavy growth (+) turned royal blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly