Hydrolysis Of Starch Pg 73-74

Question 1

Some bacteria produce amylase, which is?

Answer 1

An exoenzyme which promotes the hydrolysis of starch to sugar.

Question 2

Not all bacteria produce ________ to hydrolyze starch to sugar.

Answer 2

Amylase

Question 3

A simple starch test is used to determine the presence of _____ in the media after inoculation and incubation.

Answer 3

Starch

Question 4

_____ is added to the medium which will turn what color if starch is present.

Answer 4

Iodine
Dark purple

Question 5

If the starch has been hydrolyzed to sugar, the _____ will not change color.

Answer 5

Iodine

Question 6

Procedure: positive for amylase production =?

Answer 6

No color change (iodine color) or cleaning around streak line

Question 7

Negative for amylase production=?

Answer 7

Dark purple around streak line.

Question 8

Hydrolysis of starch data:

Answer 8

Medium:starch agar
Substrate: starch
Reagents: iodine
Pos. End product: sugar (disaccharide)
Pos. Appearance: iodine color around streak
Neg. Appearance: dark purple around streak

Question 9

Results of B. Spizizenii & E. Coli

Answer 9

Bacillus spizizenii (subtilis) was + for production of the exoenzyme amylase and therefore the hydrolysis of starch.
Escherichia coli was negative.