Detection Of Catalase Pg 77-78

Question 1

Many microorganisms are ________ and use oxygen as the final electron acceptor during cellular respiration.

Answer 1

Aerobes

Question 2

Some microorganisms do not use oxygen as the final electron acceptor, but obtain energy either by _________ or by ____________

Answer 2

Fermentation or by anaerobic respiration

Question 3

Some anaerobic microorganisms can actually be poisoned by various derivatives of oxygen including

Answer 3

H2O2 (hydrogen peroxide) and O2 (oxygen radical)

Question 4

Most microorganisms that survive in the presence of oxygen do produce the enzyme catalase causing the __________ and hence ___________ of hydrogen peroxide

Answer 4

Degradation and detoxification

Question 5

Most aerobic or facultative anaerobes can secrete

Answer 5

Catalase

Question 6

RBCs also secrete catalase in the presence of _________. But___________ do not.

Answer 6

Hydrogen peroxide. streptococci

Question 7

The maes the catalase test a good way to detect streptococcus (+/-?) from staphylococcus (+/-?)

Answer 7

Streptococcus (-)
Staphylococcus (+)

Question 8

In the procedure, adding 1-2 drops of hydrogen peroxide on the slides and observing fo the presence of _____ to indicate evidence of catalase production.

Answer 8

Oxygen bubbles

Question 9

Results: every cell + for catalase, but ______ is not

Answer 9

Streptococcus

Question 10

Why can’t you add h2O2 directly to bacterial growth on a Sheep blood agar plate?

Answer 10

Won’t be able to use culture anymore