Hydrolysis Of Gelatin Pg 75-76

Question 1

Some bacteria produce gelatinase, an exoenzyme which promotes:

Answer 1

The hydrolysis of gelatin, causing it to liquify.

Question 2

Not all arterial produce gelatinase and therefore cannot cause:

Answer 2

The liquefaction of gelatin.

Question 3

Gelatinase
Gelatin (protein polymer) ————> _____________

Answer 3

Amino acids (protein monomers)

Question 4

Gelatin medium is a liquid at temp above 25 degrees Celsius, therefore its important to refrigerate the tubes for 5-10 mins before observing and recording results in order to avoid….

Answer 4

False positives

Question 5

Hydrolysis of gelatin data:

Answer 5

Medium: gelatin tall
Substrate: gelatin
Reagents: NA
Pos. End product: amino acids
Pos. Appearance: liquid
Neg. Appearance: solid

Question 6

1. Bacillus spizizenii results:
2. Escherichia coli results:

Answer 6

1. Liquid, positive
2. Solid negative