Shanyan Chen

Question 1

Phosphorylation of ERK2 leads to the activation of its kinase activity. Contusion injury induces activation of ERK1/2 after contusive spinal cord injury (SCI).

Answer 1

T

Question 2

For the Basso, Beattie, and Bresnahan Scale Locomotor Assessment (BBB score behavior test), the lower the score, the better improvement after treatment in SCI

Answer 2

F

Question 3

What possible reasons for the marked rostral and caudal differences of tissue sparing after SCI with ERK2 shRNA injection:

Answer 3

An unequal local gain in blood perfusion; An unequal local gene knockdown in different cells; An unequal local expression/activation of ERK2 downstream targeted genes

Question 4

Which of the following statement is not true?

Answer 4

In the in vitro study, PC-12 cells were transfected by Lentivirus-mediated RNA interference ERK2 siRNA.

Question 5

Please describe the RNA interference (RNAi) in the ERK2 SCI study, and why it is important in this study?

Answer 5

1) siRNA was coded base on the ERK2 specific gene sequence.
2) siRNA was transfected directly to the cells or by Lentivirus to the animals.
3) siRNA inhibit ERK2 gene expression, by causing the destruction of specific mRNA molecules.
4) Because there is no specific pharmacologic ERK2 inhibitors, so gene based RNA inhibitor is necessary to confirm the ERK2 involvement in SCI.