Safety And Aseptic Techniques

Question 1

Gas shut off valve :Location

Answer 1

Back left corner of the room

Question 2

Fire extinguisher location

Answer 2

2; by the front doors

Question 3

Fire blanket location

Answer 3

Back of the room by equipment door

Question 4

First aid box location

Answer 4

Back of the room

Question 5

Eye wash station location

Answer 5

Back of the room in storage area

Question 6

Fume hood

Answer 6

Back right corner of the room

Question 7

What should be put onto the lab cart

Answer 7

• waste/ dirty

Flasks,slides, test tubes

Question 8

What goes onto the back counter?

Answer 8

• clean/ reusable

Prepared slides, micropipetors, tip boxes, scales

Question 9

What does the term contaminated mean

Answer 9

Unwanted microorganisms

Question 10

Where do slides and cover slips go after using them?

Answer 10

Slides ➡️ slide bucket
- REMOVE INK FIRST!!

Cover slips ➡️ broken glass box

Question 11

Brush and dust pan location

Answer 11

Behind back room door

Question 12

Where does broken glass go? What about contaminated glass?

Answer 12

Broken glass = broken glass box

Contaminated broken glass = put into biohazard bag

Question 13

TRUE or FALSE

You can pick up glass with your hands

Answer 13

False; ALWAYS use forceps

Question 14

Disinfectant (microban) locations

Answer 14

On every lab table and big container by lab cart

Question 15

What should you do when you arrive to class and what do you do when you leave?

Answer 15

Arrive ➡️ wash hands and microban table

Leave ➡️ wash hands and microban table

Question 16

When should you wash your hands?

Answer 16

Before and after class and after any spills

Question 17

Why is it important to label on the bottom where the agar is?

Answer 17

So if you lose the lid, you can still know/ identify the contents

Question 18

Where should backpacks be stored?

Answer 18

Under each lab bench; tucked away so no one trips

Question 19

How should you dress for lab?

Answer 19

• hair pulled back
• closed toe shoes
• no lose jewelry or clothes

Question 20

What should never been used/done in the lab room?

Answer 20

Eating, drinking, using cell phones, listening to music, chewing gum

Question 21

How should you carry test tubes?

Answer 21

Always!! Transport using a test tube rack

Question 22

Steps for cleaning up a bacterial spill

Answer 22

1. Inform the teacher/ lab partners & remove items out of the way
2. Circle the spill with disinfectant (microban)
3. Place dry dry paper towels on the spill
4. Drench paper towels with disinfectant (microban)
5. Using gloves, put into a biohazard bag
6. Repeat procedure
7. Wash your hands

Question 23

Aseptic techniques

Answer 23

Procedures to prevent contamination

Question 24

Contamination is

Answer 24

Any unwanted microorganisms

Question 25

What is a pure culture?

Answer 25

A culture containing only one species of microorganism; not contaminated

Question 26

Sterilization

Answer 26

The removal of all microorganisms

Question 27

What are the 4 ways to flame sterilize something?

Answer 27

1. Flame sterilize an inoculating needle or loop
2. Flame sterilize a test tube
3. Transferring bacteria from one culture tube to another
4. Transferring bacteria to solid media

Question 28

Flame sterilizing an inoculating needle or loop

Answer 28

• sterilize before use and after using*
• hold in dominant hand, move end of the wire closed to handle down to the tip until it turns red. Wait 30 seconds before using so it can cool

Question 29

Flame sterilizing a test tube

Answer 29

• using non dominant hand pick up test tube and using pinky and ring finger take the cap off w/ dominant hand. At a 45 degree angle pass top of tube through the flame then put cap back on

Question 30

Transferring bacteria from one liquid culture to another

Answer 30

• hold loop/needle in dominant hand and sterilize it
• still holding loop, pick up test tube in the non dominant hand and take cap off
• flame the opening of the test tube and insert the loop into the liquid culture making sure it’s just below the surface
• reflame the tube again and place the cap on
• pick up next tube and flame the lip
• insert the loop just below the surface (at 45 angle)
• reflame tube and put lid on and put into rack
• reflame loop/needle

Question 31

Transferring bacteria to solid media

Answer 31

• flame /sterilize loop
• pick up test tube in non dominant hand while still holding sterilizing loop and take cap off
• @45 angle run lip of tube through the flame and insert the loop just below the surface
• reflame tube and cap it putting it back into tube rack
• lightly lift petri lid and move loop gently on the surface of the agar
• close peri dish and reflame sterilizing loop