Rhabdovirus and Paramixoviruses Flashcards
what is the type of virus that rabies is
-ssrna like VSV
-non segmented
-cytoplasmic replication
what type of virus are paramyxoviruses
-ssrna like mumps and measles
-non segmented
-cytoplasmic replication
rhabdovirus glycoproteins name
G
true ro false: rabies looks like a bullet
true
true or false: measles has a thicccc membrane
false it is floppy
structural proteins of measles
HN and fusion protein
true or false: rabies a an old disease
true
First described in Mesopotamia in 2300 BC,
rabies has been recognized in humans and
animals for many centuries.
true or false: rabies is always pretty mucvh fatal
true
rabies: disease usually occures with the onset of…
Disease usually occurs with the onset of an acute
encephalomyelitis, often preceded by periods of
excitement or agitation, and quickly followed by
coma and death.
Hypersalivation and hydrophobia are prominent features oF
rabies
how do you get rabies:
you get bitten by an animal that has rabies, virus then moves up the PNS into the CNS into the brain
how did Pasteur make his rabies vaccine
-In 1881, he began studies of rabies in animals.
-He developed methods of producing attenuated virus preparations
-Progressively drying rabbit spinal cords after experimental infection
-These preparations, when inoculated into animals would protect from challenge with virulent virus without leading to the disease itself
-This was the first artificially produced virus vaccine.
-in 1885 a kiddo was treated with his vaccine and did not get rabies after getting bit by a rabid dog
Where are rabies still prevalent
in asia and in africa but in Canada there is pretty much none
-last guy in can dies in 2007
Most common reservoir of rabies
silver haired bat
Pathogenic paramyxoviruses
-Mumps virus – salivary gland and testicular infection
-Measles virus ‐ which caused around 733,000 deaths worldwide in 2000
-Respiratory syncytial virus (RSV), the major cause of bronchiolitis and pneumonia in
infants and children.
-Human parainfluenza viruses (HPIV) are the second most common causes of respiratory
tract disease in infants and children.
what are Henipaviruses
Henipaviruses are zoonotic pathogens, occurring naturally in animal hosts, but also able
to infect humans.
where do you get mumps
-PAROTID GLAND
-SUBMANDIPULAR GLAND
-subblingual gland
-salivary gland
-balls
what is the special quick to measle
RNA POL BINDS TO A CISTRON ONE AT A TIME
order of the measles proteins made
N, P, M, F, G, L
Measles:Pausing
Pausing
‐ polyadenylation at poly(U) tract
by repetitive retranscription
Attenuation
‐ polymerase falling off template
during “stuttering” process
true or false: rna pol makes subgenomic rna for rabies
true
Features that make VSV an excellent candidate as a vaccine vector
-It is a weak human pathogen does not undergo genetic recombination or genomic
reassortment and has no known transforming properties.
-VSV does not integrate any of its genomic material into host cell.
-As a vaccine strategy, VSV is known to elicit strong humoral and cellular immune
responses in vivo and naturally infects at mucosal surfaces.
-This offers an alternative less invasive intranasal route of immunization that has been
shown to induce both mucosal and systemic.
-Recombinant VSVs can be generated to accommodate large foreign gene inserts or
multiple genes into their genomes.
-VSV grows to high titers in vitro, thus facilitating rapid purification of large amounts of
virus and viral proteins.
