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MIMM 324 > Lecture 11 > Flashcards

Lecture 11 Flashcards

1
Q

What are adenoviruses responsible for?

A

respiratory infections like the cold

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2
Q

when were adenoviruses discovered

A

in the 50’s

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3
Q

What happenend when adenoviruses were introduced into rodents

A

they developed cancers

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4
Q

true or false: adenoviruses cause cancer in humans

A

false they don’t
-in rodents their genes are oncogenic because they promote growth
-in humans when the virus replicates, it kills the cell

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5
Q

who are more susceptible to adenoviruses

A

people living in close quarters like kids in boarding schools and military

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6
Q

stats about adenoviruses

A

*Non-enveloped
*Icosahedral
*90 nm in diameter
*Genome: dsDNA
35-45 kb
linear

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7
Q

which covid vaccine was due to an adenovirus?

A

astrazenequa

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8
Q

True or false: adenoviridae co-evolved with vertebrates not too long ago

A

falseeee
adenoviridae co-evolved with vertebrates for eons

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9
Q

qhich genus of adeoviridae are responsible for human infections?

A

mastadenovirus

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10
Q

Which of these are not caused by adenovirus:
Acute Respiratory Illness
Pharyngitis
Gastroenteritis
Conjunctivitis
Pneumonia
Influenza
Keratoconjunctivitis
Acute Haemorrhagic Cystitis
Hepatitis

A

influenza

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11
Q

which adenovirus serotypes are responsible for the common cold?

A

2 and 5

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12
Q

Where do adenoviruses replicate?

A

in the nucleus
it also replicates quickly

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13
Q

Where does adenoviruses replicate best?

A

in epithelial cells

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14
Q

how do we purify adenovirus

A

using a CsCl gradient and we spinnnn it

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15
Q

True or false: adenovirus has to bring lots fo proteins in order to replicate

A

false: it only brings with it the structural components and the genome

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16
Q

True or false: adenovirus is tightly packed and it is thanks to protein VII

A

true
it is like an histone like protein that condense the genome

17
Q

what is the role of protein VIII in adeno viruses

A

cements the capsid together from the inside

18
Q

hat is the role of protein VI

A

it links the core to the capsid

19
Q

True or false: the genome of adenoviruses is temporaly regulated

A

true

20
Q

what does E1A do?

A

It is a potent TF, it activated E1B, E3, E2Aand B

21
Q

how did we discover splicing?

A

with the usage of adenoviruses
-They basically looked at the EM of mRNA and saw it looping out

22
Q

Do adenoviruses have inverted terminal repeats

A

yes

23
Q

Why does a virus want oncogenes? more specifically adenoviruses

A

because iy this case epithelial cells don’t really replicate, they already are differentiated aka they don’t really divide
-viruses develop proteins that regulate replication and that will push these cells to the S phase even though they don’t have active DNA replication

24
Q

Why do adenoviruses produce an excess quantity of fibers?

A

because thet use them to bind to the CAR receptors so that they don;’t bond to themselves and block entry for the virus

25
Q

how does adenoviruses enter the cell

A

through 2 interactiosn:
-first with the car receptors
-on the penton there is this RGD loop(amino acid sequence) and this will bind to the integrins present on the cell surface
=multiple interactions

26
Q

how does adenoviruses enter the cell

A

through 2 interactiosn:
-first with the car receptors
-on the penton there is this RGD loop(amino acid sequence) and this will bind to the integrins present on the cell surface
=multiple interaction

27
Q

what causes the disassembly of the capsid

A

-acidification in the endosome activates a protease which digests protein VI=disassembly of the outer capsid

28
Q

how does adenovirus leaves the endosomelea

A

it leaves through phospholipase activity into the cytoplasm->nucleus

29
Q

what are the 2 main product of E1A

A

289R and 243R

30
Q

what is present on 289R but not 243R and what does it do

A

CR3:
-it is important for turning on the other TFs
-it binds TBP (tata box binding protein) and ATF (which binds in a sequence specific way to different promoters
when these 2 are bound, it hyperactivats the transcription of the DNA

31
Q

true or false: E1B/E3/E4 all have ATF binding sites

A

true

32
Q

E1A binds to… and … whic…

A

E1A binds tyo TBP and ATF forms like this bridge and hyperactivates the other TFS

33
Q

what do pRB,p107 and p130mbind to

A

CR1 and Cr2
E2 is responsible for binding to them
important for driving the cells to the S phase

34
Q

True or false: E1A proteins activate E2F transcription
and explain how it works

A

true
usually Rb is always bound to E2F= represses S phase genes but E1A will bind to RB and then E2F can be free to activate S phase

MIMM 324 (26 decks)

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