Respiratory Organisms Flashcards

1
Q

Lower respiratory cultures

A

BAP or CNA, Choc, MAC (not CO2), Acid Fast culture

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2
Q

Bacitracin

A

A disk for S. pyogenes sensitive, B hemolysis usually R

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3
Q

PYR

A

Enterococci/S. pyogenes, L-Pyrrolidonyl-B-naphthylamide substrate to make enzyme

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4
Q

Optochin

A

P disk, S. pneumoniae sensitive, A hemolytic Viridans resistant

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5
Q

Bile solubility

A

S. pneumoniae lyse in bile become clear, amidase, sodium deoxycholate

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6
Q

SXT PABA

A

Group A and Group B resistant

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7
Q

S pneumoniae

A

sinusitis, otitis media, bacteremia, amoxicillin

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8
Q

S. pyogenes (Group A)

A

M protein, streptolysin, scarlet/impetigo/Erysipelas/rheumatic

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9
Q

Haemophilus influenzae

A

small gray, shiny, GN short pleomorphic rods, satellitism,

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10
Q

Porphyrin test

A

ALA aminolevulinic acid into porphyrin (hemin), Wood’s lamp red/yellow (+), interchange with factor X, H. influenzae negative

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11
Q

Haemophilus test medium

A

Mueller Hinton agar/broth with X/V/yeast extract for B lactamase. K-B AST

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12
Q

Arcanobacterium haemolyticum

A

GPR diphtheroids, lymphadenopathy, small, clear B hemo, (reverse CAMP inhibit hemo)

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13
Q

Rhocococcus equi

A

GPR, rattles in foals, prolonged rhodo/pink

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14
Q

Eustachian tube

A

Auditory, connect middle ear and nasopharynx

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15
Q

Otitis media (S. pneumo/H. influ/M. cat)

A

fluid collect in middle ear because Eustachian tube inflamed (lead to eardrum rupture (treat tiny tube)

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16
Q

Otitis media treatment

A

Amoxicillin-clavulanate, oral cefuroxime axetil, IM ceftriaxone

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17
Q

Moraxella catarrhalis

A

GN diplococci, asachrolytic, Ox/Cat (+), NG MAC, DNase/butyrate esterase diff from Neisseria

18
Q

M. lacunata

A

conjunctivitis, choc pit, NG MAC, butyrate esterase

19
Q

Legionella pneumophila

A

GNR, intracellular, urine antigen test

20
Q

BYCE for legionella

A

L-cysteine (amino acid), iron salts, blue and purple, 3-5 day

21
Q

Stain for L

A

Gimenez, Gram 1% basic fuschin not safranin

22
Q

GNR K/K

A

Pseudomonas, Burkholderia, Acinetobacter, Corynebacterium, Bordetella, Stenotrophomonas, Mycoplasma

23
Q

P. aeruginosa

A

GNR, oxidizer, PB/Amino (S), use acetamide

24
Q

P. fluorescens

A

no growth 42C, no pigment, no nitrate

25
Q

P. putida

A

Gelatinase (=),

26
Q

Burkholderia cepacia CF

A

pigment, MAC pink, PB/Amino (R), SXT treatment

27
Q

OFBL

A

Oxidation fermentation polymixin bacitracin lactose agar, Burk is yellow

28
Q

Cepacia medium

A

bile salts, ticaricillin, polymyxin B, crystal violet, red +

29
Q

Burkholderia pseudomallei

A

melioidosis, silvery/wrinkled BAP, Pink MAC

30
Q

B. mallei

A

diphtheroid, glanders, catalase (+), Colistin/PB (R), gray/trans/pink MAC

31
Q

Acinetobacter baumannii (R)

A

Ox(=)/cat(+), urea/citrate(+), B lactamase, CRAB, Poly/tetra treat

32
Q

A. lwoffi (S)

A

nonutilizer, urea/citrate (=)

33
Q

ESKAPE

A

Enterococcus, S. aureus, Kle, A. baummanii, P. aeru, Enterobacter

34
Q

Corynebacterium diphtheriae

A

GPR, pseudomembrane/heart fail, Tinsdale, Cystine-Tellurite

35
Q

Loeffler’s media

A

metachromatic granules stained of C. diphtheriae with methylene blue, serum and eggs

36
Q

Elek plate

A

precipitin lines, antitoxin paper under agar

37
Q

B. pertussis

A

GN coccobacilli, NG BAP, filamentous hemagglutinin, cyto/pertussis toxin, flex swab (regan-lowe(mercury drops)/bordet-gengou(clearing))

38
Q

Bordetella bronchisepta

A

kennel cough, urease/nitrate/all (+)

39
Q

Stenotrophomonas maltophilia

A

Ox(=), transducers, lavendar/chartreuse, CR, lophotrichous

40
Q

Mycoplasma pneumoniae

A

fried egg on PPLO media,

41
Q

Chlamydophila pneumoniae

A

obligate intracellular, HELA cell line, TWAR, reticulate to elementary body

42
Q

Chlamydophila psittacii

A

exotic bird trade