Respiratory Organisms

Question 1

Lower respiratory cultures

Answer 1

BAP or CNA, Choc, MAC (not CO2), Acid Fast culture

Question 2

Bacitracin

Answer 2

A disk for S. pyogenes sensitive, B hemolysis usually R

Question 3

PYR

Answer 3

Enterococci/S. pyogenes, L-Pyrrolidonyl-B-naphthylamide substrate to make enzyme

Question 4

Optochin

Answer 4

P disk, S. pneumoniae sensitive, A hemolytic Viridans resistant

Question 5

Bile solubility

Answer 5

S. pneumoniae lyse in bile become clear, amidase, sodium deoxycholate

Question 6

SXT PABA

Answer 6

Group A and Group B resistant

Question 7

S pneumoniae

Answer 7

sinusitis, otitis media, bacteremia, amoxicillin

Question 8

S. pyogenes (Group A)

Answer 8

M protein, streptolysin, scarlet/impetigo/Erysipelas/rheumatic

Question 9

Haemophilus influenzae

Answer 9

small gray, shiny, GN short pleomorphic rods, satellitism,

Question 10

Porphyrin test

Answer 10

ALA aminolevulinic acid into porphyrin (hemin), Wood’s lamp red/yellow (+), interchange with factor X, H. influenzae negative

Question 11

Haemophilus test medium

Answer 11

Mueller Hinton agar/broth with X/V/yeast extract for B lactamase. K-B AST

Question 12

Arcanobacterium haemolyticum

Answer 12

GPR diphtheroids, lymphadenopathy, small, clear B hemo, (reverse CAMP inhibit hemo)

Question 13

Rhocococcus equi

Answer 13

GPR, rattles in foals, prolonged rhodo/pink

Question 14

Eustachian tube

Answer 14

Auditory, connect middle ear and nasopharynx

Question 15

Otitis media (S. pneumo/H. influ/M. cat)

Answer 15

fluid collect in middle ear because Eustachian tube inflamed (lead to eardrum rupture (treat tiny tube)

Question 16

Otitis media treatment

Answer 16

Amoxicillin-clavulanate, oral cefuroxime axetil, IM ceftriaxone

Question 17

Moraxella catarrhalis

Answer 17

GN diplococci, asachrolytic, Ox/Cat (+), NG MAC, DNase/butyrate esterase diff from Neisseria

Question 18

M. lacunata

Answer 18

conjunctivitis, choc pit, NG MAC, butyrate esterase

Question 19

Legionella pneumophila

Answer 19

GNR, intracellular, urine antigen test

Question 20

BYCE for legionella

Answer 20

L-cysteine (amino acid), iron salts, blue and purple, 3-5 day

Question 21

Stain for L

Answer 21

Gimenez, Gram 1% basic fuschin not safranin

Question 22

GNR K/K

Answer 22

Pseudomonas, Burkholderia, Acinetobacter, Corynebacterium, Bordetella, Stenotrophomonas, Mycoplasma

Question 23

P. aeruginosa

Answer 23

GNR, oxidizer, PB/Amino (S), use acetamide

Question 24

P. fluorescens

Answer 24

no growth 42C, no pigment, no nitrate

Question 25

P. putida

Answer 25

Gelatinase (=),

Question 26

Burkholderia cepacia CF

Answer 26

pigment, MAC pink, PB/Amino (R), SXT treatment

Question 27

OFBL

Answer 27

Oxidation fermentation polymixin bacitracin lactose agar, Burk is yellow

Question 28

Cepacia medium

Answer 28

bile salts, ticaricillin, polymyxin B, crystal violet, red +

Question 29

Burkholderia pseudomallei

Answer 29

melioidosis, silvery/wrinkled BAP, Pink MAC

Question 30

B. mallei

Answer 30

diphtheroid, glanders, catalase (+), Colistin/PB (R), gray/trans/pink MAC

Question 31

Acinetobacter baumannii (R)

Answer 31

Ox(=)/cat(+), urea/citrate(+), B lactamase, CRAB, Poly/tetra treat

Question 32

A. lwoffi (S)

Answer 32

nonutilizer, urea/citrate (=)

Question 33

ESKAPE

Answer 33

Enterococcus, S. aureus, Kle, A. baummanii, P. aeru, Enterobacter

Question 34

Corynebacterium diphtheriae

Answer 34

GPR, pseudomembrane/heart fail, Tinsdale, Cystine-Tellurite

Question 35

Loeffler's media

Answer 35

metachromatic granules stained of C. diphtheriae with methylene blue, serum and eggs

Question 36

Elek plate

Answer 36

precipitin lines, antitoxin paper under agar

Question 37

B. pertussis

Answer 37

GN coccobacilli, NG BAP, filamentous hemagglutinin, cyto/pertussis toxin, flex swab (regan-lowe(mercury drops)/bordet-gengou(clearing))

Question 38

Bordetella bronchisepta

Answer 38

kennel cough, urease/nitrate/all (+)

Question 39

Stenotrophomonas maltophilia

Answer 39

Ox(=), transducers, lavendar/chartreuse, CR, lophotrichous

Question 40

Mycoplasma pneumoniae

Answer 40

fried egg on PPLO media,

Question 41

Chlamydophila pneumoniae

Answer 41

obligate intracellular, HELA cell line, TWAR, reticulate to elementary body

Question 42

Chlamydophila psittacii

Answer 42

exotic bird trade