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Fall 2024 > GI pathogens/Blood cultures > Flashcards

GI pathogens/Blood cultures Flashcards

1
Q

Stool Viablity

A

1 hr, 72 hr fridge, Shigella/Vibrio need transport media (Cary-Blair, alkaline peptone water)

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2
Q

Media

A

BAP, MAC/EMB, HE/XLD, Campy, GN, CIN, TCBS, SMAC

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3
Q

Eosin methylene blue

A

differential, lactose fermenter (E. coli black/green, SS do not (pink/mucoid), sucrose

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4
Q

Hektoen enteric

A

lactose, sucrose, salicin, peptone metabolism by SS turn blue/green, others yellow/red

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5
Q

Xylose lysine deoxycholate

A

Salmonella turn xylose yellow then lysine to red, Shigella red (sucrose, lactose E. coli yellow)

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6
Q

CIN

A

Y. entercolitica, neutral red, cefsulodin, irgasan, novobiocin, mannitol

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7
Q

SS agar

A

lactose, neutral red, S/D

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8
Q

Thiosulfate citrate bile sucrose agar (green)

A

V. cholerae yellow, parahaemolyticus blue/green, no e coli

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9
Q

Campy agar

A

Cephalothin, charcoal

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10
Q

Selenite F broth

A

SS, NaSe inhibit GP

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11
Q

Citrobacter

A

indole+, lysine/orn=, ONPG+

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12
Q

Edwardsiella tarda

A

indole+, citrate=

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13
Q

Salmonella enterica

A

MAC (nonlactose fermenter yellow), XLD/HE H2S

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14
Q

Yersinia

A

appendicitis, paperclip, cold loving,

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15
Q

Plesiomonas shigelloides (seafood)

A

lophotrichous flagella, O/129 (S), indole+, lys/orn+, not hemolytic, no exoenzymes

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16
Q

Vibrio cholerae

A

O/129 (S), grows without salt, SUCROSE, VP+, TCBS yellow, rice water

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17
Q

V. parahaemolyticus

A

citrate=, Arabinose ferment, NaCl 8% growth, kanagawa phenomenon

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18
Q

V. vulnificus

A

lactose fermenter, immuno seafood, liver disease/diabetes

19
Q

Aeromonas hydrophilia

A

ulcer/hemorrhagic septicemia/cellulitis/ecthyma gangrenosum (pic), 5 diarrhea

20
Q

Aeromonas

A

O/129 (R), Group 1, citrate/indole/VP/lysine+, DNase/lipase/gelatinase, orn=

21
Q

TSI group 1

A

yellow/yellow, V. cholerae (MAC colorless, +everything), A. hydrophila (pink, orn=, O129 (R))

22
Q

TSI group 3

A

pink/yellow, V. parahaemolyticus (colorless, citrate/VP=)

23
Q

TSI 1 and 3

A

V. vulnificus (pink, citrate+/VP=), P. shigelloides (citrate=/gelatinase+)

24
Q

campylobacter jejuni

A

fowl/cattle water, most common gastroenteritis, 42C/microaero, cyto/enterotoxin, NG MAC, comma rods

25
Q

Helicobacter pylori

A

amoxicillin, tetracycline, metronidazole, skirrow (no cephalothin, microaero

26
Q

Rotavirus

A

fluid adsorption, <5 yr, river/tap, oral live vaccine

27
Q

Norovirus

A

cruise, fecal water or shellfish, loss of taste, most common gastroenteritis

28
Q

Adenovirus

A

respiratory, conjunctivitis, gastroenteritis in children, water diarrhea without rbc/wbc

29
Q

Sepsis

A

E. coli, S. aureus, S./K. pneumoniae, P. aeruginosa, B. fragilis, C. albicans (ESSKPBC)

30
Q

Bacteremia

A

V. strep, CNS, Clostridium, entero, Pseudomonas, enterococcus, S. aureus

31
Q

SIRS

A

systemic inflammatory response syndrome + infection = sepsis (increase breath rate, change in consciousness, low BP)

32
Q

blood culture

A

2 draw 2 set, 15 min apart, different site, before therapy, >10 ml, release CO2, SPS (anticoag/phago, inactivate therapy, inhibit Neisseria gon/menin)

33
Q

Brucella

A

milk, farm handling, joint, undulant fever, GN coccobacillus,

34
Q

Francisella tularensis, aerobe

A

tularemia, ulceroglandular (tick bite, necrotic), 14 day plate

35
Q

HACEK characteristics

A

GNB, NF oral, endocarditis, nonmotile, CO2

36
Q

HACEK

A

H. parainfluenzae, Aggregatibacter actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, Kingella sp.

37
Q

H. parainfluenzae

A

oily colonies, V factor

38
Q

Aggregatibacter actino-mycetem-comitans

A

NG MAC, star colonies, stick to side of broth

39
Q

Cardiobacterium hominis

A

aortic valve endocarditis, no fever, rosette Gram stain, agar pit

40
Q

Kingella kingae

A

pediatric joint infection/osteoarthritis, <4 yr, spreading/pitting colony, catalase = not Neisseria arthritis

41
Q

Capnocytophaga ochraecea/canimorsus

A

gliding motility, dog bite, fusiform

42
Q

Alcaligenes faecalis

A

fruity, HAI moist, gelatin+

43
Q

Achromobacter xylosoxidans/dentrificans

A

water, no pigment, citrate/nitrate+

Fall 2024 (14 decks)

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