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Fall 2024 > GI pathogens/Blood cultures > Flashcards

GI pathogens/Blood cultures Flashcards

1
Q

Stool Viablity

A

1 hr, 72 hr fridge, Shigella/Vibrio need transport media (Cary-Blair, alkaline peptone water)

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2
Q

Media

A

BAP, MAC/EMB, HE/XLD, Campy, GN, CIN, TCBS, SMAC

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3
Q

Eosin methylene blue

A

differential, lactose fermenter (E. coli black/green, SS do not (pink/mucoid), sucrose

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4
Q

Hektoen enteric

A

lactose, sucrose, salicin, peptone metabolism by SS turn blue/green, others yellow/red

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5
Q

Xylose lysine deoxycholate

A

Salmonella turn xylose yellow then lysine to red, Shigella red (sucrose, lactose E. coli yellow)

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6
Q

CIN

A

Y. entercolitica, neutral red, cefsulodin, irgasan, novobiocin, mannitol

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7
Q

SS agar

A

lactose, neutral red, S/D

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8
Q

Thiosulfate citrate bile sucrose agar (green)

A

V. cholerae yellow, parahaemolyticus blue/green, no e coli

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9
Q

Campy agar

A

Cephalothin, charcoal

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10
Q

Selenite F broth

A

SS, NaSe inhibit GP

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11
Q

Citrobacter

A

indole+, lysine/orn=, ONPG+

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12
Q

Edwardsiella tarda

A

indole+, citrate=

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13
Q

Salmonella enterica

A

MAC (nonlactose fermenter yellow), XLD/HE H2S

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14
Q

Yersinia

A

appendicitis, paperclip, cold loving,

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15
Q

Plesiomonas shigelloides (seafood)

A

lophotrichous flagella, O/129 (S), indole+, lys/orn+, not hemolytic, no exoenzymes

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16
Q

Vibrio cholerae

A

O/129 (S), grows without salt, SUCROSE, VP+, TCBS yellow, rice water

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17
Q

V. parahaemolyticus

A

citrate=, Arabinose ferment, NaCl 8% growth, kanagawa phenomenon

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18
Q

V. vulnificus

A

lactose fermenter, immuno seafood, liver disease/diabetes

19
Q

Aeromonas hydrophilia

A

ulcer/hemorrhagic septicemia/cellulitis/ecthyma gangrenosum (pic), 5 diarrhea

20
Q

Aeromonas

A

O/129 (R), Group 1, citrate/indole/VP/lysine+, DNase/lipase/gelatinase, orn=

21
Q

TSI group 1

A

yellow/yellow, V. cholerae (MAC colorless, +everything), A. hydrophila (pink, orn=, O129 (R))

22
Q

TSI group 3

A

pink/yellow, V. parahaemolyticus (colorless, citrate/VP=)

23
Q

TSI 1 and 3

A

V. vulnificus (pink, citrate+/VP=), P. shigelloides (citrate=/gelatinase+)

24
Q

campylobacter jejuni

A

fowl/cattle water, most common gastroenteritis, 42C/microaero, cyto/enterotoxin, NG MAC, comma rods

25
Helicobacter pylori
amoxicillin, tetracycline, metronidazole, skirrow (no cephalothin, microaero
26
Rotavirus
fluid adsorption, <5 yr, river/tap, oral live vaccine
27
Norovirus
cruise, fecal water or shellfish, loss of taste, most common gastroenteritis
28
Adenovirus
respiratory, conjunctivitis, gastroenteritis in children, water diarrhea without rbc/wbc
29
Sepsis
E. coli, S. aureus, S./K. pneumoniae, P. aeruginosa, B. fragilis, C. albicans (ESSKPBC)
30
Bacteremia
V. strep, CNS, Clostridium, entero, Pseudomonas, enterococcus, S. aureus
31
SIRS
systemic inflammatory response syndrome + infection = sepsis (increase breath rate, change in consciousness, low BP)
32
blood culture
2 draw 2 set, 15 min apart, different site, before therapy, >10 ml, release CO2, SPS (anticoag/phago, inactivate therapy, inhibit Neisseria gon/menin)
33
Brucella
milk, farm handling, joint, undulant fever, GN coccobacillus,
34
Francisella tularensis, aerobe
tularemia, ulceroglandular (tick bite, necrotic), 14 day plate
35
HACEK characteristics
GNB, NF oral, endocarditis, nonmotile, CO2
36
HACEK
H. parainfluenzae, Aggregatibacter actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, Kingella sp.
37
H. parainfluenzae
oily colonies, V factor
38
Aggregatibacter actino-mycetem-comitans
NG MAC, star colonies, stick to side of broth
39
Cardiobacterium hominis
aortic valve endocarditis, no fever, rosette Gram stain, agar pit
40
Kingella kingae
pediatric joint infection/osteoarthritis, <4 yr, spreading/pitting colony, catalase = not Neisseria arthritis
41
Capnocytophaga ochraecea/canimorsus
gliding motility, dog bite, fusiform
42
Alcaligenes faecalis
fruity, HAI moist, gelatin+
43
Achromobacter xylosoxidans/dentrificans
water, no pigment, citrate/nitrate+

Fall 2024 (14 decks)

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