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Fall 2024 > Mycobacterium > Flashcards

Mycobacterium Flashcards

1
Q

Decontamination

A

NALC-NaOH, mucolytic agent/decon, citrate buffer pH 6.8, refrigerated centrifuge

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2
Q

Z carbol fuschin, 3% HCL, methylene blue

A

K carbol, HCL, brilliant green, pink on blue
R-AF K perman, rapid grower no fluor

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3
Q

Lowenstein-Jensen

A

Egg, Malachite green inhibit

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4
Q

Lowenstein-Gruft

A

Nalidixic acid/penicillin inhibit non AFB

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5
Q

MGIT

A

Middlebrook broth, O2 sensitive/quenching

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6
Q

AFB ID

A

growth rate, photoreactivity, nitrate reduction, catalase, hydrolysis of Tween 80 (detergent phenol red to pink, niacin test (tb)

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7
Q

Myco

A

kansasii, marinum, scrofulaceum, gordonae, avium, intracellulare, chelonae, smegmatis, fortuitum, leprae

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8
Q

Runyon group I, Photochromogen

A

pigment after intense light

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9
Q

I kansasii

A

37C, NTM pulmonary disease not communicable (caseation)

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10
Q

I marium

A

28-30C, swimming pool granuloma

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11
Q

II Scotochromogen

A

pigment in dark

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12
Q

II scrofulaceum

A

cervical lymphadenitis in children, incise and drain

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13
Q

II gordonae

A

1-3 wk L-J, isoniazid/ethambutol (R)

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14
Q

III Non-chromogens

A

no pigment, M. intracellulare, AIDS, disseminate/caseating, middlebrook

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15
Q

III avium MAC

A

NTM pulmonary/cervical lymphadenitis in children, NG LF

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16
Q

IV Rapid growers

A

3-5 days, no AFB stain

17
Q

IV chelonae

A

wound infection after surgery/trauma in immuno, non-healing ulcer/cellulitis, peeling paper plate

18
Q

IV smegmatis

A

skin infect, orange/brown pigment, cauliflower looking on plate

19
Q

IV fortuitum

A

local cutaneous infections/abscess puncture wounds

20
Q

leprae

A

Schwann cells/peripheral nerve by laminin-binding protein, demyelination neuropathy by immune system, <37C

21
Q

Fite-Faraco stain

A

diagnose leprae on skin biopsy, micronesia/iimmuno

22
Q

MTB complex obligate pathogens

A

phagocytized, multiply in lymph node, granulomas w prominent caseous necrosis

23
Q

MTB culture

A

LJ buff colored, warty, cauliflower center, difficult to emulsify, 2-3 week, liquid growth media in automated faster

24
Q

bovis

A

3-6 weeks, dysgonic colonies, Bacille-calmette-guerin attenuated strand

25
Q

Interferon-Gamma release assays (IGRAs)

A

Quantiferon TB gold, measure amount T cell make IFN-G, (no FP with BCG) (FP for kansasii/marinum)

26
Q

Molecular NAT nucleic acid testing

A

probe ssDNA/RNA, heating and cooling dsDNA, hybridization, wash unbound probe

27
Q

Susceptibility testing

A

Anti-tubercular agents in middlebrook (isoniazid, streptomycin, p-aminosalicylic acid), molecular

28
Q

Treatment for MTB

A

isoniazid, rifampin, ethambutol, 2nd (streptomycin/aminoglycoside, p-aminosalicylic acid, kanamycin

Fall 2024 (14 decks)

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