Mycobacterium Flashcards
Decontamination
NALC-NaOH, mucolytic agent/decon, citrate buffer pH 6.8, refrigerated centrifuge
Z carbol fuschin, 3% HCL, methylene blue
K carbol, HCL, brilliant green, pink on blue
R-AF K perman, rapid grower no fluor
Lowenstein-Jensen
Egg, Malachite green inhibit
Lowenstein-Gruft
Nalidixic acid/penicillin inhibit non AFB
MGIT
Middlebrook broth, O2 sensitive/quenching
AFB ID
growth rate, photoreactivity, nitrate reduction, catalase, hydrolysis of Tween 80 (detergent phenol red to pink, niacin test (tb)
Myco
kansasii, marinum, scrofulaceum, gordonae, avium, intracellulare, chelonae, smegmatis, fortuitum, leprae
Runyon group I, Photochromogen
pigment after intense light
I kansasii
37C, NTM pulmonary disease not communicable (caseation)
I marium
28-30C, swimming pool granuloma
II Scotochromogen
pigment in dark
II scrofulaceum
cervical lymphadenitis in children, incise and drain
II gordonae
1-3 wk L-J, isoniazid/ethambutol (R)
III Non-chromogens
no pigment, M. intracellulare, AIDS, disseminate/caseating, middlebrook
III avium MAC
NTM pulmonary/cervical lymphadenitis in children, NG LF
IV Rapid growers
3-5 days, no AFB stain
IV chelonae
wound infection after surgery/trauma in immuno, non-healing ulcer/cellulitis, peeling paper plate
IV smegmatis
skin infect, orange/brown pigment, cauliflower looking on plate
IV fortuitum
local cutaneous infections/abscess puncture wounds
leprae
Schwann cells/peripheral nerve by laminin-binding protein, demyelination neuropathy by immune system, <37C
Fite-Faraco stain
diagnose leprae on skin biopsy, micronesia/iimmuno
MTB complex obligate pathogens
phagocytized, multiply in lymph node, granulomas w prominent caseous necrosis
MTB culture
LJ buff colored, warty, cauliflower center, difficult to emulsify, 2-3 week, liquid growth media in automated faster
bovis
3-6 weeks, dysgonic colonies, Bacille-calmette-guerin attenuated strand