Mycobacterium

Question 1

Decontamination

Answer 1

NALC-NaOH, mucolytic agent/decon, citrate buffer pH 6.8, refrigerated centrifuge

Question 2

Z carbol fuschin, 3% HCL, methylene blue

Answer 2

K carbol, HCL, brilliant green, pink on blue
R-AF K perman, rapid grower no fluor

Question 3

Lowenstein-Jensen

Answer 3

Egg, Malachite green inhibit

Question 4

Lowenstein-Gruft

Answer 4

Nalidixic acid/penicillin inhibit non AFB

Question 5

MGIT

Answer 5

Middlebrook broth, O2 sensitive/quenching

Question 6

AFB ID

Answer 6

growth rate, photoreactivity, nitrate reduction, catalase, hydrolysis of Tween 80 (detergent phenol red to pink, niacin test

Question 7

Myco

Answer 7

kansasii, marinum, scrofulaceum, gordonae, avium, intracellulare, chelonae, smegmatis, fortuitum, leprae

Question 8

Runyon group I, Photochromogen

Answer 8

pigment after intense light

Question 9

I kansasii

Answer 9

37C, NTM pulmonary disease not communicable (caseation)

Question 10

I marium

Answer 10

28-30C, swimming pool granuloma

Question 11

II Scotochromogen

Answer 11

pigment in dark

Question 12

II scrofulaceum

Answer 12

cervical lymphadenitis in children, incise and drain

Question 13

II gordonae

Answer 13

1-3 wk L-J, isoniazid/ethambutol (R)

Question 14

III Non-chromogens

Answer 14

no pigment, M. intracellulare, AIDS, disseminate/caseating, middlebrook

Question 15

III avium MAC

Answer 15

NTM pulmonary/cervical lymphadenitis in children, NG LF

Question 16

IV Rapid growers

Answer 16

3-5 days, no AFB stain

Question 17

IV chelonae

Answer 17

wound infection after surgery/trauma in immuno, non-healing ulcer/cellulitis, peeling paper plate

Question 18

IV smegmatis

Answer 18

skin infect, orange/brown pigment, cauliflower looking

Question 19

IV fortuitum

Answer 19

local cutaneous infections/abscess puncture wounds

Question 20

leprae

Answer 20

Schwann cells/peripheral nerve by laminin-binding protein, demyelination neuropathy by immune system, <37C

Question 21

Fite-Faraco stain

Answer 21

diagnose leprae on skin biopsy, micronesia/iimmuno

Question 22

MTB complex obligate pathogens

Answer 22

phagocytized, multiply in lymph node, granulomas w prominent caseous necrosis

Question 23

MTB culture

Answer 23

LJ buff colored, warty, cauliflower center, difficult to emulsify, 2-3 week, liquid growth media in automated faster

Question 24

bovis

Answer 24

3-6 weeks, dysgonic colonies, Bacille-calmette-guerin attenuated strand

Question 25

Tuberculin (purified protein derivative PPD) skin test

Answer 25

0.1 ml of 5 ttuberculin units, induration mm

Question 26

Interferon-Gamma release assays (IGRAs)

Answer 26

Quantiferon TB gold, 16-24 hr incubate, measure amount T cell make IFN-G,+/=/intermediate, (no FP with BCG) (FP for kansasii/marinum)

Question 27

Negative room pressure

Answer 27

isolation tech prevent cross contam, wear respirator transport

Question 28

Molecular NAT nucleic acid testing

Answer 28

probe ssDNA/RNA, heating and cooling dsDNA, hybridization, wash unbound probe

Question 29

Susceptibility testing

Answer 29

Anti-tubercular agents in middlebrook (isoniazid, streptomycin, p-aminosalicylic acid), molecular

Question 30

Treatment for MTB

Answer 30

isoniazid, rifampin, ethambutol, 2nd (streptomycin/aminoglycoside, p-aminosalicylic acid, kanamycin