Anaerobes

Question 1

1. Pasteurella multocida (Bipolar pink)

Answer 1

Pet, Glucose, NG MAC, Indole(+), mushroom smell, green brown tint

Question 2

2. Eikenella corrodens

Answer 2

Fist, pen(S), catalase (=), microaerophilic, Asacchrolytic, need heme, bleach, agarase

Question 3

3. Shewanella putrifaciens

Answer 3

stagnant water, fishy, H2S, oxidizer (K/K) red H2S, Bulla, tan/brown mucoid

Question 4

4. Vibrio vulnificus, comma shape

Answer 4

ocean, gas gangrene, large green colony

Question 5

Anaerobe media

Answer 5

CDC anaerobe media, Brucella, thioglycolate, BBE, PRAS (pre-reduced sterilized)

Question 6

Brucella agar

Answer 6

5% SB, K, hemin

Question 7

Thioglycolate

Answer 7

Resazurin indicator (pink oxidized, clear reduced), glucose, marble chip (NaHCO3)

Question 8

Bacteriodes BE

Answer 8

S/D, gentamicin inhibit aerobes, 20% bile salt inhibit anaerobes,

Question 9

Kanamycin-Vancomycin-laked blood agar (selective) LKV

Answer 9

Bacteriodes, Prevotella, Kan inhibits facilitative GNR, Van inhibit GP, Lake accelerates brown/black pigment in Prevotella

Question 10

Spore test

Answer 10

Ethanol (thio+EtOH) Heat (boil thio)

Question 11

Reverse CAMP

Answer 11

Clostridium perfringens, S agalactiae reagent

Question 12

Egg Yolk Agar

Answer 12

Lecithinase and lipase, mother of pearl, sporogenes

Question 13

Nagler Plate

Answer 13

EYA, Antitoxin half Cl. perfringens

Question 14

Clostridia GP

Answer 14

metronidazole, penicillin, antitoxins, saccholytic/proteolytic

Question 15

1. Cl. perfringens

Answer 15

Enteritis necroticans (bowel porforation), no WBC, double zone,

Question 16

Lethicinase positive

Answer 16

Cl. perfringens

Question 17

Lactose +

Answer 17

Cl. sordellii, perfringens

Question 18

Lipase +

Answer 18

C. sporogenes

Question 19

2. Cl. septicum

Answer 19

colon cancer blood culture, swarm plate, long filamentous

Question 20

3. Cl. sporogenes

Answer 20

rhizoid plate, terminal, lipase+, mother of pearl

Question 21

4. Cl. sordellii

Answer 21

irregular plate, leth/lipase =

Question 22

5. Clostridioides difficile

Answer 22

antibiotic associated diarrhea, ground glass plate, A enterotoxin, B cytotoxin, pseudomembranous colitis

Question 23

C-diff diagnosis

Answer 23

immunodiagnostic look for toxin, no t normal flora

Question 24

1. Actinomyces israelii

Answer 24

lumpy jaw syndrome, sulfur granules in pus and thio, branched GP

Question 25

2. Cutibacterium acnes

Answer 25

SBE, prosthetic joint, cornynebacterium diphtheriae like gram, day 4 contaminant

Question 26

3. Lactobacillus

Answer 26

GP pleomorphic, A hemolytic strep like on plate

Question 27

4. Mobilunicus spp.

Answer 27

GP curved rod, vaginosis

Question 28

5. Eubacterium limosum

Answer 28

periodontal disease, bite, gram/motility variable

Question 29

6. Eggerthella lenta

Answer 29

ulcerative colitis, liver/anal abscess, bacteriemia, pics

Question 30

GN Anaerobes (mucus membrahe)

Answer 30

Bacteriodes fragilis, Prevotella, Porphyromas, Fusobacterium

Question 31

1. Bacteroides fragilis group

Answer 31

no pigment, kan/col/van (R), metronidazole and BS penicillin

Question 32

2. Prevotella/Porphyromonas

Answer 32

NG BBE/bile sensitive, black pigment, brick red fluor, Por (van S)!, sininitis - brain abscess

Question 33

3. Fusobacterium spp.

Answer 33

NG BBE, no pigment, F. necrophorum (pharyngitis/large wide, gray B hemolysis on plate), F. nucletum (breadcrumb),

Question 34

Anaerobe Cocci

Answer 34

Peptostreptococcus anaerobius, Veillonella parvula

Question 35

Peptostreptococcus anaerobius

Answer 35

Reproductive tract, GP streptococci, immunocomprimised

Question 36

Veillonella parvula

Answer 36

oral, GN, inert, no ferment, Catalase =, reduce nitrate to nitrite