Anaerobes

Question 1

1. Pasteurella multocida (Bipolar pink)

Answer 1

Pet, Glucose, NG MAC, Indole(+), mushroom smell, green brown tint

Question 2

2. Eikenella corrodens

Answer 2

Fist, pen(S), catalase (=), microaerophilic, Asacchrolytic, need heme, bleach, agarase

Question 3

3. Shewanella putrifaciens

Answer 3

stagnant water, fishy, H2S, oxidizer (K/K) red H2S, Bulla, tan/brown mucoid

Question 4

4. Vibrio vulnificus, comma shape

Answer 4

ocean, gas gangrene, large green colony

Question 5

Anaerobe media

Answer 5

CDC anaerobe media, Brucella, thioglycolate, BBE, PRAS (pre-reduced sterilized)

Question 6

Brucella agar

Answer 6

5% SB, K, hemin

Question 7

Thioglycolate

Answer 7

Resazurin indicator (pink oxidized, clear reduced), glucose, marble chip (NaHCO3)

Question 8

Bacteriodes BE

Answer 8

S/D, gentamicin inhibit aerobes, 20% bile salt inhibit anaerobes,

Question 9

Kanamycin-Vancomycin-laked blood agar (selective) LKV

Answer 9

Bacteriodes, Prevotella, Kan inhibits facilitative GNR, Van inhibit GP, Lake accelerates brown/black pigment in Prevotella

Question 10

Nitrate disk

Answer 10

red pos, zinc clear pos,

Question 11

Spore test

Answer 11

Ethanol (thio+EtOH) Heat (boil thio)

Question 12

Reverse CAMP

Answer 12

Clostridium perfringens, S agalactiae reagent

Question 13

Egg Yolk Agar

Answer 13

Lecithinase and lipase, mother of pearl, sporogenes

Question 14

Nagler Plate

Answer 14

EYA, Antitoxin half Cl. perfringens

Question 15

Clostridia GP

Answer 15

metronidazole, penicillin, antitoxins, saccholytic/proteolytic

Question 16

1. Cl. perfringens

Answer 16

Enteritis necroticans (bowel porforation), no WBC, double zone,

Question 17

Lethicinase positive

Answer 17

Cl. novyi, perfringens

Question 18

Lactose +

Answer 18

Cl. sordellii, perfringens

Question 19

Lipase +

Answer 19

Cl. novyi, sporogenes

Question 20

2. Cl. septicum

Answer 20

colon cancer blood culture, swarm plate, long filamentous

Question 21

3. Cl. sporogenes

Answer 21

rhizoid plate, terminal, lipase+, mother of pearl

Question 22

4. Cl. sordellii

Answer 22

irregular plate, leth/lipase =

Question 23

5. Clostridioides difficile

Answer 23

antibiotic associated diarrhea, ground glass plate, A enterotoxin, B cytotoxin, pseudomembranous colitis

Question 24

C-diff diagnosis

Answer 24

immunodiagnostic look for toxin, no t normal flora

Question 25

GP nonspore

Answer 25

Actinomycetes israelii, Lactobacillus, Mobilunicus, Eubacterium limosum, Eggerthella lenta

Question 26

1. Actinomycetes israelii

Answer 26

lumpy jaw syndrome, sulfur granules in pus and thio, branched GP

Question 27

2. Cutibacterium acnes

Answer 27

SBE, prosthetic joint, cornynebacterium diphtheriae like gram, day 4 contaminant

Question 28

3. Lactobacillus

Answer 28

GP pleomorphic, A hemolytic strep like on plate

Question 29

4. Mobilunicus spp.

Answer 29

GP curved rod, vaginosis

Question 30

5. Eubacterium limosum

Answer 30

periodontal disease, bite, gram/motility variable

Question 31

6. Eggerthella lenta

Answer 31

ulcerative colitis, liver/anal abscess, bacteriemia, pics

Question 32

GN Anaerobes (mucus membrahe)

Answer 32

Bacteriodes fragilis, Prevotella, Porphyromas, Fusobacterium

Question 33

1. Bacteroides fragilis group

Answer 33

no pigment, kan/col/van (R), metronidazole and BS penicillin

Question 34

2. Prevotella/Porphyromonas

Answer 34

NG BBE/bile sensitive, black pigment, brick red fluor, Por (van S)!, sininitis - brain abscess

Question 35

3. Fusobacterium spp.

Answer 35

NG BBE, no pigment, F. necrophorum (pharyngitis/large wide, gray B hemolysis on plate), F. nucletum (breadcrumb),

Question 36

Anaerobe Cocci

Answer 36

Peptostreptococcus anaerobius, Veillonella parvula

Question 37

Peptostreptococcus anaerobius

Answer 37

Reproductive tract, GP streptococci, immunocomprimised

Question 38

Veillonella parvula

Answer 38

oral, GN, inert, no ferment, Catalase =, reduce nitrate to nitrite