Reproduction technologies

Question 1

AI in cattle

Answer 1

-95% of dairy cattle (mostly holstein)
-used commonly in purebred beef
-distributed frozen

Question 2

AI in swine

Answer 2

-used to a limited extent
-mainly to control disease
-distributed fresh

Question 3

AI in turkeys

Answer 3

all bred by AI

Question 4

AI in horses

Answer 4

-used by some breeds
-severe restrictions typical
-collected and used within hours

Question 5

Ai in dogs

Answer 5

-limited used mostly from problem matings

Question 6

embryo transfer

Answer 6

-super ovulate donor cow
-inseminate
-collect embryos
-transfer to recipient cows

Question 7

what stage are the embryos for transfer

Answer 7

morula or blastula

Question 8

what must the recipient of ET do to accept the embryos

Answer 8

-she must be in synchronized cycle
-with hormones
-place in the uterine horn with CL

Question 9

ET calves

Answer 9

-ET calves are full siblings
-quality recipient matters

Question 10

ET foals

Answer 10

in spring 2004 first ET foals born from CS bucking horses
-more top name mares went through super ovulation
-12 mares, 29 ova, 14 transfered, 5 pregnant

Question 11

sexing embryos

Answer 11

-cut portion off and use PCR with Y specific primers to sex
-“damaged” embryos dont freeze well
-therefore, transfer fresh

Question 12

sexing semen

Answer 12

-sorting sperm by X or Y
-flow cytometry
-50:50 to 90:10
-now on sale at AI companies