replication of DNA 1.2 Flashcards
1
Q
what is semiconservative
A
each parent strand act as a template
each new strand has one new daughter strand and a parent strand
2
Q
stages of replictaion
A
- double helix unwinds
- hydrogen bonds break and strands separate
3.primer binds to the 3’ end of template strand - free nucleotides in the cytoplasm join to base partners
- DNA polymerase ands nucleotides using complementary base pairing
- strands retwist
3
Q
what enzyme joins the dna fragments together on the lagging strand. the leading strand replicates continuously
A
ligase
4
Q
what can PCR be used for
A
solve crimes, settle paternity suits, diagnose genetic disorders
5
Q
what is PCR
A
DNA amplification
6
Q
advantages of PCR
A
highly specific
easily automated
capable of amplifying minute quantity of sample
7
Q
requirements for PCR
A
dna sample
primers
free dna nucleotides
heat tolerant dna polymerase
energy
buffer solution
8
Q
process of PCR
A
92-98 to separate strands
55-60 for primers to anneal
70-80 for DNA extension
