Replacement Organs Flashcards
Why is the need for replacement organs a universal health issue?
Organ failure and needs for organ transplants greatly exceeds the availability of organs
Strategy behind Xenotransplantation and the success rate to date
Engineer pig organs so they won’t be rejected by humans. Average survival 176 days, longest 758 days.
Interspecies blastocyst complementation
Generate human compatible organs in animals
1. KO master txn factor necessary for the development of the targeted organ
2. iPSCs from another species are induced into the host blastocyst
3. Missing organ derives from donor iPSCs
Interspecies blastocyst complementation
Issues and implications
Doesn’t work for distantly related species
Human PSCs have limited contributions to mouse, pig, and monkey
- Low efficiency of viable embryo to transfer
- Ration of pig embryos that degenerate following implantation is high
Interspecies blastocyst complementation
Ethical concerns
Some cells contribute to other lineages
- What % chimerism is too much?
