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Hematology 2 > RBC, WBC, Counting Chamber (RVSP) > Flashcards

RBC, WBC, Counting Chamber (RVSP) Flashcards

0
Q

7 characteristics of a good RBC diluting fluid

A
  • isotonic solution
  • has a high specific gravity
  • easy to prepare
  • cheap
  • good preservative
  • has a buffer action
  • must not initiate the growth of molds
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1
Q

The red count is

A

Number of red cells in 1 cu mm of blood

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2
Q

Dilution in RBC count

A

1:200

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3
Q

Number of drops discarded in the RBC pipet

A

First 5-6 drops

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4
Q

Angle for charging in RBC ct

A

30-35°

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5
Q

Presence of fluid on the moats of the counting chamber

A

Overcharging

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6
Q

Failure to cover the entire ruled area of the counting chamber

A

Undercharging

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7
Q

Number of mins to allow the RBCs to settle

A

5-10 mins

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8
Q

Objective used to initially focus the ruled area in RBC counting

A

LPO

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9
Q

Objective used for actual red cell counting

A

HPO

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10
Q

Cell difference bet. 2 squares in RBC counting

A

20 or less

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11
Q

Best RBC diluting fluid

A

Dacie’s fluid/Formol citrate

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12
Q

Composition of Dacie’s fluid

A

40% soln of formaldehyde

3% W/V trisodium citrate

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13
Q

RBC diluting fluid; allows the growth of yeasts and produces clumping of cells in patients suffering from liver cirrhosis

A

Hayem’s

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14
Q

Composition of Hayem’s fluid

A

Mercuric chloride
Sodium sulfate (crystalline/anhydrous)
Sodium chloride
Distilled water

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15
Q

RBC diluting fluid; prevents rouleaux formation, precipitates proteins in cases of hemoglobinuria and hyperglobulinemia

A

Gower’s

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16
Q

Composition of Gower’s fluid

A

Glacial acetic acid
Sodium sulfate (crystalline)
Distilled water

Sodium chloride
Sodium sulfate
Glycerine
Distilled water

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17
Q

RBC diluting fluid; supports the growth of fungi

A

Toisson’s

18
Q

Composition of Toisson’s fluid

A 
Sodium chloride
Sodium sulfate
Glycerine
Methyl violet
Distilled water
19
Q

Composition of Bethell’s fluid (RBC diluting fluid)

A 
Sodium sulfate
Sodium chloride
Glycerine
Sodium merthiolate
Distilled water
20
Q

RBC diluting fluid; used in cases of emergency

A

Normal saline solution (NSS) or Physiologic Saline solution

21
Q

Composition of NSS

A

Sodium chloride

Distilled water

22
Q

Composition of 3.8% sodium citrate (RBC diluting fluid)

A

Sodium citrate

Distilled water

23
Q

First model of Coulter Counter

A

Coulter Counter Model S

24
Q

Coulter counter; + RDW

A

Coulter counter model S Plus

25
Q

Coulter counter; histogram differential

A

Coulter Counter Model S Plus IV

26
Q

Coulter counter; 90 Sx/hr

A

Coulter Counter Model S Plus II

27
Q

Coulter counter; 115 Sx/hr

A

Coulter Counter Model S Plus III

28
Q

Coulter counter; blood sample volume reduced to 100 uL instead of 1 mL

A

Coulter Counter Model S Plus IV

29
Q

Coulter counter; automated ca piercer

A

Coulter Counter Model S Plus V

30
Q

Coulter Counter; autosampler with 2 rotating trays that can hold 32 tubes each

A

Coulter Counter Model S Plus VI

31
Q

Coulter Counter; capable of accepting and mixing 144 Sx at one time in preparation for Sx identification, aspiration, and analysis

A

Coulter Counter Model S Plus STKR

32
Q

Technicon; 60 Sx/hr

A

Technicon H-1

33
Q

Technicon; 100 Sx/hr

A

Technicon H-2

34
Q

The white cell count is

A

The number of white cells in 1 cumm of blood

35
Q

Characteristics of a good WBC diluting fluid

A
  • hypotonic solution
  • easily prepared
  • cheap
  • readily available
  • good preservative
36
Q

Dilution for WBC ct

37
Q

Number of drops discarded from the WBC pipet

38
Q

Objective used for WBC counting

39
Q

Cell diference between squares in WBC count

A

12 cells or less

40
Q

Composition of 1-3% Acetic acid with Gentian violet (WBC diluting fluid)

A

Glacial acetic acid
Gentian violet
Distilled water

41
Q

Composition of 1% HCl (WBC diluting fluid)

A

Hydrochloric acid

Distilled water

42
Q

Composition of Tuerk’s fluid (WBC diluting fluid)

A

Glacial acetic acid
Methyl violet
Distilled water

Hematology 2 (12 decks)

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