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Hematology 2 > Lab Compre (RVSP) > Flashcards

Lab Compre (RVSP) Flashcards

0
Q

Veins for venipuncture

A

Cephalic vein
Basilic vein
Median cubital vein

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1
Q

Ideal puncture depth

A

2-3 mm

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2
Q

Inches above the site the tourniquet is applied

A

3-4 in

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3
Q

Angle in venipuncture

A

15°

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4
Q

Ideal venipuncture depth

A

0.5 - 1.0 cm

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5
Q

Order of draw accdg to color

A 
Yellow
Light blue
Red
Green
Lavender
Gray
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6
Q

Oder of draw accdg to function

A 
Blood culture
Coagulation
Serology/BB
Plasma chemistry
Blood cell counting
Glucose
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7
Q

Order of draw accdg to additives

A 
ACD & SPS
Sodium citrate
None
Sodium/lithium heparin
K2EDTA
Sodium fluoride-potassium oxalate
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8
Q

Additive; inhibits thrombin formation to prevent clotting

A

Sodium heparin/Lithium heparin

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9
Q

Needle angle for arterial puncture

A

35-40°

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10
Q

Most popular method for smear preparatiom

A

Two-slide/Wedge method

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11
Q

Angle of spreader slide in smear prep

A

30-45°

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12
Q

4 criterion for a good smear

A
  • even transition from thick to thin area
  • must cover 2/3 or 3/4 of the slide
  • smooth and even surface, free from ridges, waves, or holes
  • feathery edge
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13
Q

Other mtds for smear prep

A
  • Two-cover slip/Ehrlich’s Two-cover glass method
  • Spinner’s method
  • Automated smear preparation
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14
Q

Instrument used for Spinner’s mtd of smar prep

A

Hemaspinner

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15
Q

Method for staining blood smear

A

Staining jar/Dip method

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16
Q

Stains in the Staining jar/dip method

A

Methanol
Eosin
Methylene blue
Buffer solution/aged distilled water

17
Q

4 mtds for differential counting

A

Four-field meander
Two-field
Exaggerated battlement
Strip differential

18
Q

6 stations in the Hemastainer Automatic Slide Stainer

A
  • Methanol
  • Wright’s or Wright-Giemsa stain
  • Stain-Buffer mixture (Wright’s or Wright-Giemsa + Phosphate buffer)
  • Deionized water
  • Phosphate buffer
  • Warm air
19
Q

Mtd of staining; blood smear is placed on a rack positioned in a dish

A

Staining Dish Mtd

20
Q

Contained in the Stain-Pak

A

Stain
Buffer
Rinse solution

21
Q

Staining mtd; uses Stain-Pak

A
  • Hema-Tek 1000 Slide Stainer

- Hema-Tek 2000 Slide Stainer

22
Q

Stain for diff ct; contains methylene blue or its oxidative product like Azure B; also contains Eosin B or Eosin Y

A

Romanowsky Stains

23
Q

Most satisfactory type of Romanowsky stain for general routine hematologic studies

A

Wright’s stain

24
Q

Composition of Wright’s stain

A

Oxidized methylene blue

Eosin azures

25
Q

Stain for demonstration of inclusion bodies and intracellular parasites aside from staining WBCs

A

Giemsa stain

26
Q

Composition of Giemsa stain

A

Eosin Y with Azure blue

Methylene blue in methanol with glycerin

27
Q

Stain for diff ct; similar to Wright’s stain except for the mtd used to oxidize methylene blue

A

Leishman, Jenner, and May-Grunwald

28
Q

Stains that consist of Romanowsky stain and another dye

A

Panoptic stains

29
Q

Types of panoptic stains

A

Wright’s-Giemsa
Jenner-Giemsa
May-Grunwald-Giemsa

30
Q

Hb is measures as

A

Oxyhemoglobin

31
Q

Indirect mtds for measuring Hb

A
  • Acid Hematin mtd

- Cyanmethemoglobin (MHbCN) mtd or Hemoglobin Cyanide (HiCN) mtd

32
Q

Reagent in Acid Hematin mtd for Hb

A

0.1 N HCl

33
Q

Color of soln in Acid Hematin Mtd

A

Yellowish Brown

34
Q

Reagent used in the Cyanmethemoglobin mtd or Hemoglobin cyanide mtd for Hb

A

Drabkin’s reagent

35
Q

Composition of Drabkin’s reagent

A
  • potassium ferricyanide
  • potassium cyanide
  • sodium bicarbonate
36
Q

Best manual metd for Hb determination

A

Cyanmethemoglobin or Hemoglobin cyanide mtd

37
Q

Form of Hb not converted to cyanmethemoglobin by Drabkin’s reagent

A

Sulfhemoglobin

38
Q

Wavelength in cyanmethemoglobin mtd

A

540 nm

39
Q

Direct matching mtds for Hb

A

Tallquist or Scale
Dares Hemoglobinometer
Alkaline Hematin

40
Q

Direct mtds for Hb

A
  • Oxyhemoglobin (Sodium Carbonate and Photoelectric Oxyhemoglobin)
  • Carboxyhemoglobin

Hematology 2 (12 decks)

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