Lab Compre (RVSP) Flashcards
Veins for venipuncture
Cephalic vein
Basilic vein
Median cubital vein
Ideal puncture depth
2-3 mm
Inches above the site the tourniquet is applied
3-4 in
Angle in venipuncture
15°
Ideal venipuncture depth
0.5 - 1.0 cm
Order of draw accdg to color
Yellow Light blue Red Green Lavender Gray
Oder of draw accdg to function
Blood culture Coagulation Serology/BB Plasma chemistry Blood cell counting Glucose
Order of draw accdg to additives
ACD & SPS Sodium citrate None Sodium/lithium heparin K2EDTA Sodium fluoride-potassium oxalate
Additive; inhibits thrombin formation to prevent clotting
Sodium heparin/Lithium heparin
Needle angle for arterial puncture
35-40°
Most popular method for smear preparatiom
Two-slide/Wedge method
Angle of spreader slide in smear prep
30-45°
4 criterion for a good smear
- even transition from thick to thin area
- must cover 2/3 or 3/4 of the slide
- smooth and even surface, free from ridges, waves, or holes
- feathery edge
Other mtds for smear prep
- Two-cover slip/Ehrlich’s Two-cover glass method
- Spinner’s method
- Automated smear preparation
Instrument used for Spinner’s mtd of smar prep
Hemaspinner
Method for staining blood smear
Staining jar/Dip method
Stains in the Staining jar/dip method
Methanol
Eosin
Methylene blue
Buffer solution/aged distilled water
4 mtds for differential counting
Four-field meander
Two-field
Exaggerated battlement
Strip differential
6 stations in the Hemastainer Automatic Slide Stainer
- Methanol
- Wright’s or Wright-Giemsa stain
- Stain-Buffer mixture (Wright’s or Wright-Giemsa + Phosphate buffer)
- Deionized water
- Phosphate buffer
- Warm air
Mtd of staining; blood smear is placed on a rack positioned in a dish
Staining Dish Mtd
Contained in the Stain-Pak
Stain
Buffer
Rinse solution
Staining mtd; uses Stain-Pak
- Hema-Tek 1000 Slide Stainer
- Hema-Tek 2000 Slide Stainer
Stain for diff ct; contains methylene blue or its oxidative product like Azure B; also contains Eosin B or Eosin Y
Romanowsky Stains
Most satisfactory type of Romanowsky stain for general routine hematologic studies
Wright’s stain
Composition of Wright’s stain
Oxidized methylene blue
Eosin azures
Stain for demonstration of inclusion bodies and intracellular parasites aside from staining WBCs
Giemsa stain
Composition of Giemsa stain
Eosin Y with Azure blue
Methylene blue in methanol with glycerin
Stain for diff ct; similar to Wright’s stain except for the mtd used to oxidize methylene blue
Leishman, Jenner, and May-Grunwald
Stains that consist of Romanowsky stain and another dye
Panoptic stains
Types of panoptic stains
Wright’s-Giemsa
Jenner-Giemsa
May-Grunwald-Giemsa
Hb is measures as
Oxyhemoglobin
Indirect mtds for measuring Hb
- Acid Hematin mtd
- Cyanmethemoglobin (MHbCN) mtd or Hemoglobin Cyanide (HiCN) mtd
Reagent in Acid Hematin mtd for Hb
0.1 N HCl
Color of soln in Acid Hematin Mtd
Yellowish Brown
Reagent used in the Cyanmethemoglobin mtd or Hemoglobin cyanide mtd for Hb
Drabkin’s reagent
Composition of Drabkin’s reagent
- potassium ferricyanide
- potassium cyanide
- sodium bicarbonate
Best manual metd for Hb determination
Cyanmethemoglobin or Hemoglobin cyanide mtd
Form of Hb not converted to cyanmethemoglobin by Drabkin’s reagent
Sulfhemoglobin
Wavelength in cyanmethemoglobin mtd
540 nm
Direct matching mtds for Hb
Tallquist or Scale
Dares Hemoglobinometer
Alkaline Hematin
Direct mtds for Hb
- Oxyhemoglobin (Sodium Carbonate and Photoelectric Oxyhemoglobin)
- Carboxyhemoglobin