Exp 2 - smear Prep Flashcards
Simplest and most popular method of smear preparation
1 slide for smear and 1 slide for spreader
Two slide of wedge method
Most appropriate specimen for smear prep technique
Peripheral blood
Angle of smearing
30-45
Good smear/
Thick to thin area
2/3 or 3/4 of the slide
Even surface
Feathery edge
3 other methods of smear prep
2 cover slip or Ehelich two cover glass method
Spinner’s method
Automated smear prep
Spinners method is done using an instrument called
Hema spinners
How many drops of blood will u place in the middle of the slide (Hemaspinner method)
3-4 drops
2 instruments in automated smear preparation
Sysmex SP-1000i
Beckman Coulter blood analyEr
For automated slide making and staining of blood.
- Computer based results
- dependent on the HEMATOCRIT value
Sysmex sp-1000i
How many seconds can the smear be prepared in Sysmex SP-1000i
Every 30 secs