HEMA LAB 13-16 Flashcards
How much NaCl do you dissolve in 100 mL of d.water (OFT)
0.5gm
color of partial hemolysis
faintly pink
color of complete hemolysis
red with no sediments
normally, hemolysis begins at what tube?
Tube 17
What kind of method did we perform in the laboratory for OFT
griffin-sanford method
other methods of OFT
- rapid screening test
- dacie’s method
- quantitative test
- unopette method
in the rrapid screening test of OFT, place 5 mL of 0.5% ____ _______ solution in a small test tube
sodium chloride
True or false. If there is hemolysis in the rapid screening, we proceed with a confirmatory test
true
In Dacie;s method, blood is diluted with what kind of solution (OFT)
isotonic saline
In Dacie;s method, blood is buffered at what pH with phosphate
7.4
In Dacie;s method, degree of hemolysis is determined on the basis of what kind of hemoglobin
free hemoglobin,
how many tubes for dacie’s method
12
time and temp for dacie’s method
30 minutes, room temp
absorbance to be used (dacie’s method)
545 nm
what is used as blank in the dacie’s method
supernatant from 0.85% saline soln
what method of OFT is used for a definitve diagnosis of hereditary spherocytosis?
quantitative test
what are carefully controlled and frequently required in the quantitative test
salt concentration and pH
method of OFT that involves the addition of blood into prepared vials containing varying concentration of NaCl
Unopette MEthod
true or false. red cells will begin to hemolyze as the %age of NaCl decreases
false…increases dapat
sino asawa ni donald duck?
dacie duck…
measures the ability of small blood vessels to control bleeding after injury
bleeding time
a measure of the function of platelets as well as the integrity of the vessel wall
duration of bleeding
what can hasten the bleeding time in duke’s method
when the filter paper touches the wound (so do not allow it)
blot the blood using filter paper every how many seconds
30
when do you stop timing
when the bleeding ceases
normal value for duke’s method
2-4 minutes
method of bleeding time when you use a sphygmomanometer cuff
ivy’s
inflate the sphygmomanometer cuff at what pressure
40 mmHg
true or false. you should decrease the pressure of the sphygmomanometer cuff as the procedure progresses
false. maintain it at 40 mmHg
what part of the forearm do you cleanse with 70% alcohol (ivy’s)
volar surface
true or false. the area selected for ivy’s method should be full of veins
FALSE
depth of the three successive punctures (ivy’s)
2-3mm
true or false. Ivy’s method of the interval of blotting the blood with filter paper is the same as duke’s method
true. same na 30s
normal value of ivy’s method
1-7 minutes
what method of bleeding time are you using when a 6mm puncture is made
copley-lalitch method
immerse the punctured finger at what kind of solution that is warmed at 37 degrees Celsius
sterile physiologic saline solution
normal value of copley-lalitch method
heart heart…. <3 minutes
measures the period required for the free flowing blood to clot or solidify after it has been removed from the body
clotting time
another term for clotting time
coagulation time
capillary tube used in DALE and LAIDLAWS method
nonheparinized
when do u start timing in DALE and LAIDLAWS method
as soon as blood enters the tube
set the capillary tube at what position for 2 minutes
horizontal
another name for DALE and LAIDLAWS method
capillary tube method
true or false. break off about 1 cm of the capillary tube at 60 second interval until fibrin thread bridges the broken ends of the capillary tube
false. 30 seconds lng
method that we used in the laboratory
LEE and WHITE method
how many tubes for lee and white method
3 tubes
how many mL of blood do we need for lee and white method
3mL
temperature of water bath for lee and white method
37 degrees Celsius..pwd rin hawakan ntin with own hands kasi un nman body temp ntin
method of coagulation time where you draw the tip of the lancet across the drop of blood at 30 second interval
drop or slide method
prothrombin time is frequently used to monitor what theraphy
oral anticoagulant theraphy
what factors are inhibited by oral anticoagulants
FActors II, V, VII and X (2,5,7,10)
what kind of prothrombin time is seen in DIC, specific couagulation deficiencies, dysproteinemias, etc.
Prolonged PT
what is the preferred anticoagulant for PT
sodium citrate
the test for PT should be performed within how many hours after blood collection
2
color ng citrated tube
light blue (tama ba?)
centrifuge at what rpm at how many minutes (PT)
1 500 rpm for 5 minutes
what are you going to put in a clean dry tube after centrifugation (PT) …plasma or serum?
plasma
Pt is essentially a test of the (a. intrinsic b. extrinsic) pathway of clotting time and the common pathway
extrinsic
PT is least sensitive to what factor
factor II
the plasma clotting time obtained when an excess of thromboplastin and optimum calcium are added to citrated plasma
Prothrombin time (PT)
thromboplastin reagent consists of
- lyophilized extract of rabbit brain
- calcium chloride
