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Hematology 2 > HEMA LAB 13-16 > Flashcards

HEMA LAB 13-16 Flashcards

1
Q

How much NaCl do you dissolve in 100 mL of d.water (OFT)

A

0.5gm

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2
Q

color of partial hemolysis

A

faintly pink

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3
Q

color of complete hemolysis

A

red with no sediments

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4
Q

normally, hemolysis begins at what tube?

A

Tube 17

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5
Q

What kind of method did we perform in the laboratory for OFT

A

griffin-sanford method

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6
Q

other methods of OFT

A
  • rapid screening test
  • dacie’s method
  • quantitative test
  • unopette method
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7
Q

in the rrapid screening test of OFT, place 5 mL of 0.5% ____ _______ solution in a small test tube

A

sodium chloride

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8
Q

True or false. If there is hemolysis in the rapid screening, we proceed with a confirmatory test

A

true

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9
Q

In Dacie;s method, blood is diluted with what kind of solution (OFT)

A

isotonic saline

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10
Q

In Dacie;s method, blood is buffered at what pH with phosphate

A

7.4

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11
Q

In Dacie;s method, degree of hemolysis is determined on the basis of what kind of hemoglobin

A

free hemoglobin,

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12
Q

how many tubes for dacie’s method

A

12

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13
Q

time and temp for dacie’s method

A

30 minutes, room temp

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14
Q

absorbance to be used (dacie’s method)

A

545 nm

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15
Q

what is used as blank in the dacie’s method

A

supernatant from 0.85% saline soln

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16
Q

what method of OFT is used for a definitve diagnosis of hereditary spherocytosis?

A

quantitative test

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17
Q

what are carefully controlled and frequently required in the quantitative test

A

salt concentration and pH

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18
Q

method of OFT that involves the addition of blood into prepared vials containing varying concentration of NaCl

A

Unopette MEthod

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19
Q

true or false. red cells will begin to hemolyze as the %age of NaCl decreases

A

false…increases dapat

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20
Q

sino asawa ni donald duck?

A

dacie duck…

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21
Q

measures the ability of small blood vessels to control bleeding after injury

A

bleeding time

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22
Q

a measure of the function of platelets as well as the integrity of the vessel wall

A

duration of bleeding

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23
Q

what can hasten the bleeding time in duke’s method

A

when the filter paper touches the wound (so do not allow it)

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24
Q

blot the blood using filter paper every how many seconds

A

30

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25
Q

when do you stop timing

A

when the bleeding ceases

26
Q

normal value for duke’s method

A

2-4 minutes

27
Q

method of bleeding time when you use a sphygmomanometer cuff

A

ivy’s

28
Q

inflate the sphygmomanometer cuff at what pressure

A

40 mmHg

29
Q

true or false. you should decrease the pressure of the sphygmomanometer cuff as the procedure progresses

A

false. maintain it at 40 mmHg

30
Q

what part of the forearm do you cleanse with 70% alcohol (ivy’s)

A

volar surface

31
Q

true or false. the area selected for ivy’s method should be full of veins

A

FALSE

32
Q

depth of the three successive punctures (ivy’s)

A

2-3mm

33
Q

true or false. Ivy’s method of the interval of blotting the blood with filter paper is the same as duke’s method

A

true. same na 30s

34
Q

normal value of ivy’s method

A

1-7 minutes

35
Q

what method of bleeding time are you using when a 6mm puncture is made

A

copley-lalitch method

36
Q

immerse the punctured finger at what kind of solution that is warmed at 37 degrees Celsius

A

sterile physiologic saline solution

37
Q

normal value of copley-lalitch method

A

heart heart…. <3 minutes

38
Q

measures the period required for the free flowing blood to clot or solidify after it has been removed from the body

A

clotting time

39
Q

another term for clotting time

A

coagulation time

40
Q

capillary tube used in DALE and LAIDLAWS method

A

nonheparinized

41
Q

when do u start timing in DALE and LAIDLAWS method

A

as soon as blood enters the tube

42
Q

set the capillary tube at what position for 2 minutes

A

horizontal

43
Q

another name for DALE and LAIDLAWS method

A

capillary tube method

44
Q

true or false. break off about 1 cm of the capillary tube at 60 second interval until fibrin thread bridges the broken ends of the capillary tube

A

false. 30 seconds lng

45
Q

method that we used in the laboratory

A

LEE and WHITE method

46
Q

how many tubes for lee and white method

A

3 tubes

47
Q

how many mL of blood do we need for lee and white method

A

3mL

48
Q

temperature of water bath for lee and white method

A

37 degrees Celsius..pwd rin hawakan ntin with own hands kasi un nman body temp ntin

49
Q

method of coagulation time where you draw the tip of the lancet across the drop of blood at 30 second interval

A

drop or slide method

50
Q

prothrombin time is frequently used to monitor what theraphy

A

oral anticoagulant theraphy

51
Q

what factors are inhibited by oral anticoagulants

A

FActors II, V, VII and X (2,5,7,10)

52
Q

what kind of prothrombin time is seen in DIC, specific couagulation deficiencies, dysproteinemias, etc.

A

Prolonged PT

53
Q

what is the preferred anticoagulant for PT

A

sodium citrate

54
Q

the test for PT should be performed within how many hours after blood collection

A

2

55
Q

color ng citrated tube

A

light blue (tama ba?)

56
Q

centrifuge at what rpm at how many minutes (PT)

A

1 500 rpm for 5 minutes

57
Q

what are you going to put in a clean dry tube after centrifugation (PT) …plasma or serum?

A

plasma

58
Q

Pt is essentially a test of the (a. intrinsic b. extrinsic) pathway of clotting time and the common pathway

A

extrinsic

59
Q

PT is least sensitive to what factor

A

factor II

60
Q

the plasma clotting time obtained when an excess of thromboplastin and optimum calcium are added to citrated plasma

A

Prothrombin time (PT)

61
Q

thromboplastin reagent consists of

A
  • lyophilized extract of rabbit brain

- calcium chloride

Hematology 2 (12 decks)

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