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Hematology 2 > Hematology Lab 1-3 > Flashcards

Hematology Lab 1-3 Flashcards

1
Q

Peripheral blood is obtained from

A

3rd or 4th finger

earlobe (adults)

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2
Q

Squeezing of site of puncture will lead to

A

alteration of the composition of blood

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3
Q

difficulty in obtaining blood can be relieved by using

A

warm compress

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4
Q

necessary for tests that require anticoagulation

A

venipuncture

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5
Q

why is veni the most convenient method of blood collection

A

reduces the number and variety of apparatus carried during collection

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6
Q

apply the tourniquet ____ inches above the site of punture

A

3-4 inches

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7
Q

attach the syringe at a ____ angle

A

15

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8
Q

depth a vein should be punctured

A

0.5-1 cm

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9
Q

if the blood is anticoagulated, mix the tube by

A

gentle inversion

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10
Q

use of plain red evac tube

A
  • blood chem
  • serology
  • bb
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11
Q

use of lavender evac tube

A

blood cell counting

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12
Q

use of light blue evac tube

A

coagulation studies

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13
Q

use of green evac tube

A

plasma chemistry

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14
Q

use of gray evac tube

A

glucose

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15
Q

use of yellow evac tube

A
  • blood culture
  • bb studies
  • HLA phenotyping
  • DNA and paternity testing
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16
Q

use of royal blue evac tube

A
  • toxicology

- detection of trace metals

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17
Q

use of red marbled gold (hemogard) evac tube

A

blood chemistry

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18
Q

use of light green marbled, light green (hemogard) evac tube

A

potassium determination

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19
Q

use of yellow marbled orange evac tube

A

STAT chemistry

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20
Q

use of tan evac tube

A

lead determination

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21
Q

use of pink evac tube

A

whole blood hematology test

BB

22
Q

peripheral blood from infants is obtained from

A

the heel or big toe

23
Q

baby emman’s foot is held firmly between the ______ and the _____ of dianne’s working hand

A

thumb and index finger

24
Q

recommended site of puncture on newborns

A

heel only

25
Q

what procedure is done when the infant is wrapped in a sheet so that the arms are immovable

A

external jugular vein puncture infants

26
Q

are thin flexible hollow tubes inserted and positioned onto a vein in order to access the bloodstream for the delivery of theraphies

A

Central Venous Access Device

27
Q

type of CVAD that have one end positioned externally

A

cathether

28
Q

type of CVAD that are surgically placed inernally requiring the use of a special type of needle

A

ports

29
Q

angle of arterial puncture

A

35-40

30
Q

prevention of hemolysis during blood collection (venipuncture)

A
  • use sharp smooth needle
  • puncture the vein directly w/o searching
  • tourniquet must not be too tight
  • aspirate blood gently
  • gentle inversion
  • if serum is need, do not rim and centrifuge the blood until the clot has formed
31
Q

simplest most popular method of blood collection

A

two-slide or wedge method

32
Q

criteria of good smear

A

you know what it is black and yellow black and yellow

33
Q

angle of spreader slide

A

30-45

34
Q

procedure na may sixteen sided figure (smear prep)

A

two-cover slip/ehrlichs two cover glass method

35
Q

the rseultant silde in sipnner’s method is covered with

A

thin monolayer of cells

36
Q

two automated machines used in smear prep

A

sysmex SP-1000i

Beckman Coulter Blood Analyzer

37
Q

how many secs/sex do you dip the basic dye in diff ct

A

4 seconds

38
Q

ph ng buffer soln (diff ct)

A

7.2

39
Q

WBC with a nucleus that is usually indistinct and obscured by granules

A

Basophil

40
Q

WBC with a nuclues that is spongy and sprawling with brain like convolutions

A

monocyte

41
Q

four shapes ng neutrophilic band

A

C, S, U, horse-shoe shaped

42
Q

second most abundant WBC

A

lymphocyte

43
Q

methods of diff ct

A

four field meander
two-field
exaggerated battlement
strip differential

44
Q

tawag sa stain, buffer, and rinse solns

A

Stain-Pak

45
Q

buffer used in the hemasteiner automatic slide stainer

A

phosphate buffer

46
Q

oxidative pdt of methylene blue

A

azure blue

47
Q

the dyes produced in this stain are considered polychromatic

A

romanowsky stain

48
Q

most satisfactory in general routine hematologic studies

A

wright’s

49
Q

Romanowsky stain + another dye

A

panoptic stain

50
Q

stain similar to wright’s except for the method to oxidize methylene blue

A

leishman, jenner, and may grunwald

51
Q

stain for the demonstration of inclusion bodies and intracellular parasites

A

giemsa stain

Hematology 2 (12 decks)

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