Hematology Lab 1-3 Flashcards
Peripheral blood is obtained from
3rd or 4th finger
earlobe (adults)
Squeezing of site of puncture will lead to
alteration of the composition of blood
difficulty in obtaining blood can be relieved by using
warm compress
necessary for tests that require anticoagulation
venipuncture
why is veni the most convenient method of blood collection
reduces the number and variety of apparatus carried during collection
apply the tourniquet ____ inches above the site of punture
3-4 inches
attach the syringe at a ____ angle
15
depth a vein should be punctured
0.5-1 cm
if the blood is anticoagulated, mix the tube by
gentle inversion
use of plain red evac tube
- blood chem
- serology
- bb
use of lavender evac tube
blood cell counting
use of light blue evac tube
coagulation studies
use of green evac tube
plasma chemistry
use of gray evac tube
glucose
use of yellow evac tube
- blood culture
- bb studies
- HLA phenotyping
- DNA and paternity testing
use of royal blue evac tube
- toxicology
- detection of trace metals
use of red marbled gold (hemogard) evac tube
blood chemistry
use of light green marbled, light green (hemogard) evac tube
potassium determination
use of yellow marbled orange evac tube
STAT chemistry
use of tan evac tube
lead determination
use of pink evac tube
whole blood hematology test
BB
peripheral blood from infants is obtained from
the heel or big toe
baby emman’s foot is held firmly between the ______ and the _____ of dianne’s working hand
thumb and index finger
recommended site of puncture on newborns
heel only
what procedure is done when the infant is wrapped in a sheet so that the arms are immovable
external jugular vein puncture infants
are thin flexible hollow tubes inserted and positioned onto a vein in order to access the bloodstream for the delivery of theraphies
Central Venous Access Device
type of CVAD that have one end positioned externally
cathether
type of CVAD that are surgically placed inernally requiring the use of a special type of needle
ports
angle of arterial puncture
35-40
prevention of hemolysis during blood collection (venipuncture)
- use sharp smooth needle
- puncture the vein directly w/o searching
- tourniquet must not be too tight
- aspirate blood gently
- gentle inversion
- if serum is need, do not rim and centrifuge the blood until the clot has formed
simplest most popular method of blood collection
two-slide or wedge method
criteria of good smear
you know what it is black and yellow black and yellow
angle of spreader slide
30-45
procedure na may sixteen sided figure (smear prep)
two-cover slip/ehrlichs two cover glass method
the rseultant silde in sipnner’s method is covered with
thin monolayer of cells
two automated machines used in smear prep
sysmex SP-1000i
Beckman Coulter Blood Analyzer
how many secs/sex do you dip the basic dye in diff ct
4 seconds
ph ng buffer soln (diff ct)
7.2
WBC with a nucleus that is usually indistinct and obscured by granules
Basophil
WBC with a nuclues that is spongy and sprawling with brain like convolutions
monocyte
four shapes ng neutrophilic band
C, S, U, horse-shoe shaped
second most abundant WBC
lymphocyte
methods of diff ct
four field meander
two-field
exaggerated battlement
strip differential
tawag sa stain, buffer, and rinse solns
Stain-Pak
buffer used in the hemasteiner automatic slide stainer
phosphate buffer
oxidative pdt of methylene blue
azure blue
the dyes produced in this stain are considered polychromatic
romanowsky stain
most satisfactory in general routine hematologic studies
wright’s
Romanowsky stain + another dye
panoptic stain
stain similar to wright’s except for the method to oxidize methylene blue
leishman, jenner, and may grunwald
stain for the demonstration of inclusion bodies and intracellular parasites
giemsa stain
