HEMA LAB 17-20 Flashcards
preferred anticoagulant for APTT
sodium citrate
same lng ng pt ung rpm at number of minutes sa centrifuge
1 500 rpm for 15 mins
APTT can measure platelets and factor XIII
false
what factors can APTT measure?
factors VIII, IX, XI…also Prekallikren or FLetcher
what therapy can APPT monitor
heparin theraphy
used to measure the availability of functional fibrinogen
thrombin time
when is TT prolonged?
cases of low levels of fibrinogen
- fibrinogen function is impaired
- presence of heparin, fibrin/fibrinogen products and thrombolytics agents(i.e. streptokinase)
a soluble plasma protein that is instrumental in the normal coagulation process
fibrinogen
another name for fibrinogen
factor I
is added to undiluted plasma
low potency thrombin
thrombin reagent consists of
- thrombin time reagent (bovine)
- lyophilisate for 1mL
end products of thrombin clotting assays
insoluble fibrin polymers
basahin niyo nlng ung two stages sa lab manual (TT ito)
ANG INIT DITO SA DORM SHET
what do you pour in a wassermann tube up to about one inch from the rim (CRT)
castor oil
what do you use to aspirate blood up to the 20 cu mm mark (CRT)
sahli’s pipette
what kind of formation do you want to observe on the surface of the drop of blood
formation of dimpling or nipple like protrusion
indicates the endpoint of HIRSCHBOECK method
formation of dimpling or nipple like protrusion
another name for HIRSCHBOECK method
castor oil method
is stefanini or test tube method a qualitative or a quantitative test?
quantitative
where do you place 3-5 ml of venous blood (stefanini method)
chemically clean wassermann tube
do you use a water bath in the test tube method? (CRT)
yes
observe the tube at what succession of hours? (CRT)
1, 2, 16, 18, 24 hours
what to do when blood adheres to the wall of the tube (CRT)
rim the clot with a stiff straight wire to detach the clot from the wall of the tube
Retraction begins within how many hours
1 hour
Retraction usually ends within how many hours
18-24 hours
what method of CRT are you using when there is a glass rod and a cork
Mac Farlane
examine the blood for evidence of coagulation at what minute interval ? (CRT)
5-10 mins
where do you place the sphygmomanometer cuff in the HESS test?
around the arm just above the elbow
midway between the systolic and the diastolic pressure for 5 minutes
Quick’s
35 mm Hg for 15 minutes
Gothlin’s
how many minutes that have to elapse before you count the number of tiny spots
15-30 minutes
grade of 21-50 petechiae
3+
grade of 11-20 petechiae
2+
number of petechiae in 4+
50 and above
HESS test is also called?
Tourniquet test or RUMPEL-LEEDES test
normal result of the HESS test
1+ (1-10 petechiae)
other method of CRT that uses negative pressure of sufficiant degree to the skin and indirectly to the vascular tissue
suction or petechiometer
normal value of the petechiometer method
> 4 petechiae at 200 mm Hg
