RBC development & morphology Flashcards
1
Q
Stages of erythropoiesis (when using Romanowsky stain) (6)
A
- Pronormoblast
- Basophilic normoblast
- Polychromatophilic normoblast (basophilic nuclei)
- Orthochromatic normoblast (basophilic nuclei)
- Polychromatophilic erythrocyte (anuclear)
- Mature red blood cells (anuclear)
2
Q
Predominant site of erythropoiesis at different ages
A
- Embryo => yolk sac
- Foetus => liver
- Immature => bone marrow (widespread)
- Mature bone marrow of few locations (e.g. ends of long bones)
3
Q
Discuss how automated analysers may detect and
represent various RBC morphologies. Include any limitations*.
A
RBC histogram. Limitations*
4
Q
Difference b/w polychromatophilic RBC and Reticulocyte
A
- Polychrom: use Romanowski stain done on dried, fixed blood = bluish hue (shades)
- Reticulocyte: use Supravital stain (new methylene blue, crystal violet ) and incubate w/ living cells = shows aggregates of RNA
5
Q
increase [reticulocytes] indicates
A
increased erythropoeisis
6
Q
- decrease SA: Vol ratio
- no/little central pallor
- may be congenital or aquired
(usually indicates)
A
spherocyte
bc haemolytic anaemia
7
Q
- decrease Vol: SA ratio
- bc dehydrated = spikes/crenation
A
echinocyte
8
Q
- increase SA => irregular foldings
- bc abnormal lipid content in membrane
A
acanthocyte
9
Q
- Hb concentrated at poles
- looks like a canoe
A
eliptocyte
10
Q
- increase SA: Vol ratio
- pale areas contain low Hb
- looks like a target
A
codocyte / target cell
11
Q
a) large RBC
b) small RBC
A
a) macrocyte
b) microcyte
12
Q
How do immature RBC lose their nucleus? and at the end of the process what does this result to
A
- RBC surround macrophage = erythroblastic island
- macrophage feed iron to RBC & remove nucleus
Results to: - reticulocyte (anucleated)
- pyrenocyte (nucleus w/ lil cytoplasm) => phagocytosed by macrophages
