RBC

Question 1

• Developing nucleated RBC precursor with normal appearance
• Restricted to the bone marrow

Answer 1

• Erythroblast (RBC precursor)
• Aka: Normoblast

Question 2

STAGES OF ERYTHROID PRECURSOR MATURATION

Answer 2

1. Over all diameter of the cell decreases
2. Diameter of the nucleus decreases more rapidly than the diameter of the cell
3. Nuclear chromatin pattern becomes coarser, clumped and condensed
4. Nuclei Disappear
5. Cytoplasm changes from blue to gray-blue to salmon pink

Question 3

Primary oxygen-sensing system

Answer 3

Kidney(Peritubular Fibroblast):

Question 4

• Major stimulatory cytokine for RBC

Answer 4

EPO (ERYTHROPOIETIN)

Question 5

• Thermostable, nondialyzeble, glycoportein hormone

Answer 5

EPO (ERYTHROPOIETIN)

Question 6

EPO

• Consist of a

Answer 6

carbohydrate unit

termina sialic acid unit

Question 7

EPO ACTION:
Growth factor (cytokine) that initiates intracellular message to developing
erythroid cells =

Answer 7

SIGNAL TRANSDUCTION

Question 8

= increased cell division and maturation, increased intestinal iron absorption and hemoglobin synthesis and more RBCs entering the circulation

Answer 8

• EPO + EPOR

Question 9

EPO (ERYTHROPOIETIN)
• 3 MAJOR EFFECTS:

Answer 9

1. Allowing early release of reticulocytes from the bone marrow
2. Preventing apoptotic cell death
3. Reducing the time needed for cells to mature in the bone marrow.

Question 10

THERAPEUTIC USES OF EPO
• Produced by recombinant technology- ______ and ______
• Stimulates______ and _____ by acting to specific receptors in the BM
• Anemias of chronic infection
• Anemias resulting from chemotherapy
• Anemias secondary to malignancy; associated with HIV
• Anemias in premature infants
• Autologous blood transfusion; After BM transplant

Answer 10

EPOITIN alpha & EPOITIN beta

RBC proliferation and differentiation