quiz pero parang quiz bee daw HAHAHAHA Flashcards
single celled living
organisms
BACTERIA
The beneficial functions of microbes (4)
Lactobacillus
Penicillium
Saccharomyces cerevisae
fusarium strain flavolapis
To maintain gut health and keep your body running optimally
Lactobacillus
this type of fungus with antibacterial properties that led to the first mass-produced antibiotic
Penicillium
It is also the same microbe used in nutritional yeast, a nutrient rich savory vegan supplement
Saccharomyces cerevisae
with origin in yellowstone, natures fynd ferments this microbe to make Fy
fusarium strain flavolapis
causes strep throat
Streptococcus
Staphylococcus: cause
staph infections
causes E. coli infections
Escherichia coli
These bacteria are the most common BLANK responsible for BLANK , or blood poisoning.
GERM and SEPTICIMIA
- Bacteria in blood can spread and lead to BLANK, a systemic overreaction to wide spread infection in your body.
sepsis
Bacteria in urine that cause urinary tract infections.
Aerococcus urinae
Bacteria that cause a sexually transmitted infection (STI) called
Chlamydia trachomatis
Bacteria that cause whooping cough
Bordetella pertussis
A method that allows the multiplication of bacterial cells in or on a culture medium under controlled laboratory conditions.
bacterial culture
enables genome sequencing of these strains
Bacterial culture
enables the study of the BLANK and is the first step in establishing recommendations for effective treatment.
antibiotic susceptibility of bacteria
Most bacteria can grow to some extent in the presence of oxygen, known as
aerobic culture
Species found in atmospheric conditions, such as on the skin surface or in the upper respiratory tract, will typically grow well in the presence of
oxygen.
Species that are naturally found in low oxygen environments, such as in deep wounds or abscesses or the deep ocean, will typically grow best in the absence of oxygen
anaerobic culture
some cannot grow in the presence of oxygen at all, and these are called
obligate anaerobes
switching from aerobic respiration to fermentation or anaerobic respiration if oxygen is absent, are called
facultative anaerobes
CLASSIFICATIONS OF MEDIA (4)
Complete media
Minimal media
Defined media
Undefined media
can be helpful when trying to bulk up a pure culture and get the bacterial cells in good condition.
Complete media
supply only the bare necessities for survival and can be useful in manipulating which pathways are turned on in the bacterium.
Minimal media
all the ingredients are known
Defined media
tend to contain complex mixtures of nutrients and chemical species in unknown proportions, such as yeast extract.
Undefined media
A culture medium is simply water
and nutrients that support
microbial growth.
Bacterial culture methods
3 primary categories of culture media
NUTRITIVE
DIFFERENTIAL
SELECTIVE
Media that support
the growth of many different
microorganisms without
distinguishing genera or species
NUTRITIVE
allow several different types of bacteria to grow, but also contain compounds that allow microbial genera
DIFFERENTIAL
are used to select for the growth of a particular “selected” microorganism.
SELECTIVE
Bacterial culture methods (3)
Culture broth
Storing bacterial cells
Nutrient agar
Culture in liquid media, also known as a BLANK, gives the bacteria present easy access to the available nutrients compared to static bacterial colonies.
Culture broth
When looking to store bacterial strains long term, they may be grown up in liquid media. Glycerol is then added, which will prevent complete freezing and consequent lysis of the bacterial cells, permitting their storage at
-80 °C.
Adding agar to BLANK enables it to be set in petri dishes, as slopes or in plugs.
liquid media
is useful when you wish to select individual colonies from a mixed culture
Solid media
Common problems with bacterial culture (5)
Contamination
Overgrowth of some species
Antibiotic treatment prior to sampling
Incorrect growth conditions
Non-culturable and slow-growing organisms
Good aseptic technique can help to avoid contamination of bacterial cultures.
Contamination
Some bacterial species grow easily and vigorously
Overgrowth of some species
In a diagnostic setting, it is important to know if antibiotic treatment has been administered prior to sampling.
Antibiotic treatment prior to sampling
The use of inappropriate or suboptimal growth conditions may impede or completely prevent the growth of your target strain.
Incorrect growth conditions
Some bacterial species, even now, cannot be grown in the lab.
Non-culturable and slow-growing organisms
Applications of bacterial culture (5)
Diagnose infection
Genetic manipulation
Epidemiological study
Scale up to enable omics studies
Develop vaccines and therapeutics
cells are grown under BLANK, generally outside their natural environment
controlled conditions
macroscopically visible
collection of millions of bacteria originating from a single bacterial cell
Colony
introduced this medium
Used for preparing solid medium obtained from seaweeds
Franny Hesse
Types of Culture media
Based on their consistency (3)
solid medium
liquid medium
semi solid medium
Types of Culture media
Based on the constituents ingredients (4)
simple medium
complex medium
synthetic or defined medium
Special media
special media (8)
◦Enriched media
◦Enrichment media
◦Selective media
◦Indicator media
◦Differential media
◦Sugar media
◦Transport media
◦Media for biochemical reactions
Based on Oxygen requirement (2)
Aerobic media
Anaerobic media
contains 2% agar
Colony morphology, pigmentation, hemolysis can be appreciated.
Solid media
no agar. For inoculum preparation, Blood culture, for the isolation of pathogens from a mixture. Eg: Nutrient broth
Liquid media
0.5% agar. Eg: Motility medium
Semi solid medium
Media other than basal media. They have added ingredients. Provide special nutrients
Complex media
Media prepared from pure chemical substances and its exact composition is known
Synthetic or defined media
Substances like blood, serum, egg
are added to the basal medium
Enriched media
Used to grow bacteria that are exacting in their nutritional n
Enriched media
Liquid media used to isolate
pathogens from a mixed
culture.
Enrichment media
is incorporated with
inhibitory substances to
suppress the unwanted organism.
Media
These media contain an indicator which changes its colour when a bacterium grows in the
Indicator media
Media used for transporting the samples.
Transport media
These media are used to grow anaerobic
organisms.
Anaerobic media
Culture methods include: (7)
Streak culture
Lawn culture
Stroke culture
Stab culture
Pour plate Method
Liquid culture
Anaerobic culture methods
Used for the isolation of bacteria in pure culture
from clinical specimens.
STREAK CULTURE
Provides a uniform surface growth of the
bacterium.
LAWN CULTUR
e is made in tubes
containing agar slope / slant.
STROKE CULTURE
Provide a pure growth of
bacterium for slide
agglutination and other
diagnostic tests.
STROKE CULTURE
Prepared by puncturing a suitable medium – gelatin or glucose agar with a long, straight, charged wire
STAB CULTURE
Agar medium is melted (15 ml) and cooled to 4
POUR PLATE METHOD
are inoculated by
touching with a charged loop or by adding the inoculum with pipettes or
syringes.
LIQUID CULTURE