quiz pero parang quiz bee daw HAHAHAHA Flashcards

1
Q

single celled living
organisms

A

BACTERIA

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2
Q

The beneficial functions of microbes (4)

A

Lactobacillus
Penicillium
Saccharomyces cerevisae
fusarium strain flavolapis

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3
Q

To maintain gut health and keep your body running optimally

A

Lactobacillus

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4
Q

this type of fungus with antibacterial properties that led to the first mass-produced antibiotic

A

Penicillium

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5
Q

It is also the same microbe used in nutritional yeast, a nutrient rich savory vegan supplement

A

Saccharomyces cerevisae

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6
Q

with origin in yellowstone, natures fynd ferments this microbe to make Fy

A

fusarium strain flavolapis

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7
Q

causes strep throat

A

Streptococcus

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8
Q

Staphylococcus: cause

A

staph infections

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9
Q

causes E. coli infections

A

Escherichia coli

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10
Q

These bacteria are the most common BLANK responsible for BLANK , or blood poisoning.

A

GERM and SEPTICIMIA

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11
Q
  • Bacteria in blood can spread and lead to BLANK, a systemic overreaction to wide spread infection in your body.
A

sepsis

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12
Q

Bacteria in urine that cause urinary tract infections.

A

Aerococcus urinae

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13
Q

Bacteria that cause a sexually transmitted infection (STI) called

A

Chlamydia trachomatis

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14
Q

Bacteria that cause whooping cough

A

Bordetella pertussis

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15
Q

A method that allows the multiplication of bacterial cells in or on a culture medium under controlled laboratory conditions.

A

bacterial culture

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16
Q

enables genome sequencing of these strains

A

Bacterial culture

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17
Q

enables the study of the BLANK and is the first step in establishing recommendations for effective treatment.

A

antibiotic susceptibility of bacteria

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18
Q

Most bacteria can grow to some extent in the presence of oxygen, known as

A

aerobic culture

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19
Q

Species found in atmospheric conditions, such as on the skin surface or in the upper respiratory tract, will typically grow well in the presence of

A

oxygen.

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20
Q

Species that are naturally found in low oxygen environments, such as in deep wounds or abscesses or the deep ocean, will typically grow best in the absence of oxygen

A

anaerobic culture

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21
Q

some cannot grow in the presence of oxygen at all, and these are called

A

obligate anaerobes

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22
Q

switching from aerobic respiration to fermentation or anaerobic respiration if oxygen is absent, are called

A

facultative anaerobes

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23
Q

CLASSIFICATIONS OF MEDIA (4)

A

Complete media
Minimal media
Defined media
Undefined media

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24
Q

can be helpful when trying to bulk up a pure culture and get the bacterial cells in good condition.

A

Complete media

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25
Q

supply only the bare necessities for survival and can be useful in manipulating which pathways are turned on in the bacterium.

A

Minimal media

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26
Q

all the ingredients are known

A

Defined media

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27
Q

tend to contain complex mixtures of nutrients and chemical species in unknown proportions, such as yeast extract.

A

Undefined media

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28
Q

A culture medium is simply water
and nutrients that support
microbial growth.

A

Bacterial culture methods

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29
Q

3 primary categories of culture media

A

NUTRITIVE
DIFFERENTIAL
SELECTIVE

30
Q

Media that support
the growth of many different
microorganisms without
distinguishing genera or species

A

NUTRITIVE

31
Q

allow several different types of bacteria to grow, but also contain compounds that allow microbial genera

A

DIFFERENTIAL

32
Q

are used to select for the growth of a particular “selected” microorganism.

A

SELECTIVE

33
Q

Bacterial culture methods (3)

A

Culture broth
Storing bacterial cells
Nutrient agar

34
Q

Culture in liquid media, also known as a BLANK, gives the bacteria present easy access to the available nutrients compared to static bacterial colonies.

A

Culture broth

35
Q

When looking to store bacterial strains long term, they may be grown up in liquid media. Glycerol is then added, which will prevent complete freezing and consequent lysis of the bacterial cells, permitting their storage at

A

-80 °C.

36
Q

Adding agar to BLANK enables it to be set in petri dishes, as slopes or in plugs.

A

liquid media

37
Q

is useful when you wish to select individual colonies from a mixed culture

A

Solid media

38
Q

Common problems with bacterial culture (5)

A

Contamination

Overgrowth of some species

Antibiotic treatment prior to sampling

Incorrect growth conditions

Non-culturable and slow-growing organisms

39
Q

Good aseptic technique can help to avoid contamination of bacterial cultures.

A

Contamination

40
Q

Some bacterial species grow easily and vigorously

A

Overgrowth of some species

41
Q

In a diagnostic setting, it is important to know if antibiotic treatment has been administered prior to sampling.

A

Antibiotic treatment prior to sampling

42
Q

The use of inappropriate or suboptimal growth conditions may impede or completely prevent the growth of your target strain.

A

Incorrect growth conditions

43
Q

Some bacterial species, even now, cannot be grown in the lab.

A

Non-culturable and slow-growing organisms

44
Q

Applications of bacterial culture (5)

A

Diagnose infection

Genetic manipulation

Epidemiological study

Scale up to enable omics studies

Develop vaccines and therapeutics

45
Q

cells are grown under BLANK, generally outside their natural environment

A

controlled conditions

46
Q

macroscopically visible
collection of millions of bacteria originating from a single bacterial cell

A

Colony

47
Q

introduced this medium
Used for preparing solid medium obtained from seaweeds

A

Franny Hesse

48
Q

Types of Culture media
Based on their consistency (3)

A

solid medium
liquid medium
semi solid medium

49
Q

Types of Culture media
Based on the constituents ingredients (4)

A

simple medium

complex medium

synthetic or defined medium

Special media

50
Q

special media (8)

A

◦Enriched media
◦Enrichment media
◦Selective media
◦Indicator media
◦Differential media
◦Sugar media
◦Transport media
◦Media for biochemical reactions

51
Q

Based on Oxygen requirement (2)

A

Aerobic media
Anaerobic media

52
Q

contains 2% agar
Colony morphology, pigmentation, hemolysis can be appreciated.

A

Solid media

53
Q

no agar. For inoculum preparation, Blood culture, for the isolation of pathogens from a mixture. Eg: Nutrient broth

A

Liquid media

54
Q

0.5% agar. Eg: Motility medium

A

Semi solid medium

55
Q

Media other than basal media. They have added ingredients. Provide special nutrients

A

Complex media

56
Q

Media prepared from pure chemical substances and its exact composition is known

A

Synthetic or defined media

57
Q

Substances like blood, serum, egg
are added to the basal medium

A

Enriched media

58
Q

Used to grow bacteria that are exacting in their nutritional n

A

Enriched media

59
Q

Liquid media used to isolate
pathogens from a mixed
culture.

A

Enrichment media

60
Q

is incorporated with
inhibitory substances to
suppress the unwanted organism.

A

Media

61
Q

These media contain an indicator which changes its colour when a bacterium grows in the

A

Indicator media

62
Q

Media used for transporting the samples.

A

Transport media

63
Q

These media are used to grow anaerobic
organisms.

A

Anaerobic media

64
Q

Culture methods include: (7)

A

Streak culture
Lawn culture
Stroke culture
Stab culture
Pour plate Method
Liquid culture
Anaerobic culture methods

65
Q

Used for the isolation of bacteria in pure culture
from clinical specimens.

A

STREAK CULTURE

66
Q

Provides a uniform surface growth of the
bacterium.

A

LAWN CULTUR

67
Q

e is made in tubes
containing agar slope / slant.

A

STROKE CULTURE

68
Q

Provide a pure growth of
bacterium for slide
agglutination and other
diagnostic tests.

A

STROKE CULTURE

69
Q

Prepared by puncturing a suitable medium – gelatin or glucose agar with a long, straight, charged wire

A

STAB CULTURE

70
Q

Agar medium is melted (15 ml) and cooled to 4

A

POUR PLATE METHOD

71
Q

are inoculated by
touching with a charged loop or by adding the inoculum with pipettes or
syringes.

A

LIQUID CULTURE