Quiz #3 Week 8

Question 1

The Kirby Bauer technique is: The most common procedure used to determine the effectiveness of ______________ for __________ or ___________ the growth of an ________________

Answer 1

antibacterial medicines (antibiotics), killing, inhibiting, infecting bacteria

Question 2

Purpose of the Kirby-Bauer Technique:

Answer 2

To determine the sensitivity of a bacterium to several antibacterial medicines

Question 3

(Kirby Bauer Technique) Paper disks are impregnated with different ___________

Answer 3

antibacterial medicines.

Question 4

Which agar plate is used in the Kirby Bauer Technique?

Answer 4

Mueller-Hilton agar plate

Question 5

Has the plate been heavily inoculated with bacteria

Answer 5

yes

Question 6

The bacteria will grow into what is called a “ “

Answer 6

lawn

Question 7

(Kirby Bauer Technique) During incubation, the medicine diffuses out from the disk, the _______ or ________ of the bacterial cells in the lawn may be affected.

Answer 7

growth, survival

Question 8

A zone of ________ _________ may appear around the disk.

Answer 8

growth inhibition

Question 9

The diameter of this zone indicates __________ or _______ to the tested bacterium to the antibacterial medicine.

Answer 9

susceptibility, resistance

Question 10

Antibacterial medicine in disk diffuses _______ degrees and affects bacterial growth

Answer 10

360

Question 11

How many hours old is the bacterial culture in the broth tube

Answer 11

18 hours old

Question 12

Press swab against the inside of the tube to _________

Answer 12

remove excess medium

Question 13

Inoculate the entire surface of the agar with a ________ spaced _____ _____ _____ motion

Answer 13

closely, back-and-forth

Question 14

How many times is the agar surface inoculated?

Answer 14

3

Question 15

This technique results in a “ “ of bacteria on the agar surface

Answer 15

lawn

Question 16

How far should the disks be from the plate edge? and how far from other disks

Answer 16

20mm, 25mm

Question 17

Sterilize the forceps by first _______ it in _________ and then _________

Answer 17

dipping, alcohol, burning off the alcohol

Question 18

(Kirby Bauer Technique) Place plate upside-down in the incubator, and incubate it at _______ degrees for _______

Answer 18

35 degrees C, 16-18 hours

Question 19

For both techniques - After incubation measure the diameter in ______

Answer 19

mm

Question 20

For both techniques - When measuring place the ruler on the _______ of the plate

Answer 20

underside

Question 21

For both techniques - What should you do if the alcohol in the beaker ignites?

Answer 21

Cover the beaker immediately to block air and douse the flames.

Question 22

What is the name of the technique in testing antiseptics and disinfectants

Answer 22

Disk Diffusion Technique

Question 23

Disk diffusion technique purpose:To evaluate the ________ of selected ___________ in affeting the growth of a particular __________

Answer 23

effectiveness, antibacterial chemicals (antiseptics and disinfectants) , bacterium

Question 24

In the disk diffusion technique ________ paper disks that have been dipped in different ________ ______ are placed in a freshly -prepared ________of bacteria

Answer 24

absorbant, chemical solutions, lawn

Question 25

What type of alcohol is used for both disk diffusion and the kirby-bauer technique?

Answer 25

95% ethanol

Question 26

How thick should the Mueller-Hinton agar plate be?

Answer 26

4mm

Question 27

During disk diffusion technique incubation, as the chemical diffuses it may affect the ______ of the bacteria creating a ___________________

Answer 27

zone of growth inhibition

Question 28

(Disk Diffusion Technique) Results will indicate only if a particular __________ will adversely affect the ________ of a given _______

Answer 28

chemical, growth, bacterium

Question 29

Allow the inoculated surface to dry for ________

Answer 29

5min in the manual it says 10min??

Question 30

What is the incubation time and temperature for disk diffusion technique?

Answer 30

35 degrees C for 18-24hrs

Question 31

Place container containing alcohol _______ from the open flame

Answer 31

away

Question 32

What agar plate is used for the disk diffusion technique in Lammert? What agar plate was used in the lab?

Answer 32

Mueller-Hinton, TSA

Question 33

In disk diffusion technique; the edge of the paper disk is dipped into _________/________ which allows for _________ action to saturate the disk

Answer 33

antiseptic/disinfectant, capillary