Quiz #3 Week 8 Flashcards
HD
The Kirby Bauer technique is: The most common procedure used to determine the effectiveness of ______________ for __________ or ___________ the growth of an ________________
antibacterial medicines (antibiotics), killing, inhibiting, infecting bacteria
Purpose of the Kirby-Bauer Technique:
To determine the sensitivity of a bacterium to several antibacterial medicines
(Kirby Bauer Technique) Paper disks are impregnated with different ___________
antibacterial medicines.
Which agar plate is used in the Kirby Bauer Technique?
Mueller-Hilton agar plate
Has the plate been heavily inoculated with bacteria
yes
The bacteria will grow into what is called a “ “
lawn
(Kirby Bauer Technique) During incubation, the medicine diffuses out from the disk, the _______ or ________ of the bacterial cells in the lawn may be affected.
growth, survival
A zone of ________ _________ may appear around the disk.
growth inhibition
The diameter of this zone indicates __________ or _______ to the tested bacterium to the antibacterial medicine.
susceptibility, resistance
Antibacterial medicine in disk diffuses _______ degrees and affects bacterial growth
360
How many hours old is the bacterial culture in the broth tube
18 hours old
Press swab against the inside of the tube to _________
remove excess medium
Inoculate the entire surface of the agar with a ________ spaced _____ _____ _____ motion
closely, back-and-forth
How many times is the agar surface inoculated?
3
This technique results in a “ “ of bacteria on the agar surface
lawn
How far should the disks be from the plate edge? and how far from other disks
20mm, 25mm
Sterilize the forceps by first _______ it in _________ and then _________
dipping, alcohol, burning off the alcohol
(Kirby Bauer Technique) Place plate upside-down in the incubator, and incubate it at _______ degrees for _______
35 degrees C, 16-18 hours
For both techniques - After incubation measure the diameter in ______
mm
For both techniques - When measuring place the ruler on the _______ of the plate
underside
For both techniques - What should you do if the alcohol in the beaker ignites?
Cover the beaker immediately to block air and douse the flames.
What is the name of the technique in testing antiseptics and disinfectants
Disk Diffusion Technique
Disk diffusion technique purpose:To evaluate the ________ of selected ___________ in affeting the growth of a particular __________
effectiveness, antibacterial chemicals (antiseptics and disinfectants) , bacterium
In the disk diffusion technique ________ paper disks that have been dipped in different ________ ______ are placed in a freshly -prepared ________of bacteria
absorbant, chemical solutions, lawn
What type of alcohol is used for both disk diffusion and the kirby-bauer technique?
95% ethanol
How thick should the Mueller-Hinton agar plate be?
4mm
During disk diffusion technique incubation, as the chemical diffuses it may affect the ______ of the bacteria creating a ___________________
zone of growth inhibition
(Disk Diffusion Technique) Results will indicate only if a particular __________ will adversely affect the ________ of a given _______
chemical, growth, bacterium
Allow the inoculated surface to dry for ________
5min in the manual it says 10min??
What is the incubation time and temperature for disk diffusion technique?
35 degrees C for 18-24hrs
Place container containing alcohol _______ from the open flame
away
What agar plate is used for the disk diffusion technique in Lammert? What agar plate was used in the lab?
Mueller-Hinton, TSA
In disk diffusion technique; the edge of the paper disk is dipped into _________/________ which allows for _________ action to saturate the disk
antiseptic/disinfectant, capillary
