Q 10 practical on enzymes (12)

Question 1

Formula for Q10 coefficient:

Answer 1

Rate of reaction at temperature T + 10 /
Rate of reaction at temperature T

Question 2

Control variables:

Answer 2

-Volume and concentration of enzyme solution (catalase) measured with syringe
-Volume of hydrogen peroxide
-pH controlled by buffer solution
-Time that measurements for release of oxygen are taken

Question 3

Devise a procedure to test hypothesis that “increased temperature increases initial rate of reaction of catalase in bananas”

Answer 3

1)Same source/age of bananas
2)Same volume of hydrogen peroxide solution
3)Using 5 different temperatures (10,20,30,40,50)
4)Measure oxygen released with a gas syringe
5)Repeat 3 times at each temperature and calculate initial rate of reaction from graph