Identify plant stems (6)

Question 1

What stain can be used?

Answer 1

Methylene blue

Question 2

Control variable:

Answer 2

Stem from same source, same age and mass
Same diameter of vessel

Question 3

Method:

Answer 3

1)Cut thin cross section of stem with scalpel
2)Add these sections to stain, leave it there for 5 minutes
3)Rinse excess stain off with water, or use filter paper, add it to a slide
4)Covering it with a cover slip, slowly to avoid trapping air bubbles which could be mistaken for plant tissues
5)View under a light microscope and adjust the focus to form a clear image