Pyruvate Dehydrogenase Complex

Question 1

where does PDC take place?

Answer 1

in mitochondria (pyruvate transported here)

Question 2

PDC converts ___ to ___

Answer 2

pyruvate; acetyl coA

Question 3

Acetyl CoA involved in both ___ and ___ reactions; it is an ___ molecule

Answer 3

anabolic; catabolic; energy–>i –

Question 4

The 8 electrons from the citric acid cycle come from how many reduced NADH and FADH2?

Answer 4

3 NADH, 1 FADH2

Question 5

All these reactions happen in the __ of the mitochondria, except for one in the ____

Answer 5

matrix; inner membrane

Question 6

how many enzymes make up the PDH complex?

Answer 6

3

Question 7

what are the 3 prosthetic groups of the enzymes in PDH complex?

Answer 7

TPP, lipoate, FAD

Question 8

what are the 2 coenzymes in this complex?

Answer 8

NAD and CoA-SH

Question 9

what is Enzyme 1?

Answer 9

pyruvate dehydrogenase

Question 10

what is Enzyme 2?

Answer 10

dihydrolipoyl transacetylase

Question 11

what is enzyme 3?

Answer 11

dihydrolipoyl dehydronase

Question 12

how many cofactors in total?

Answer 12

5

Question 13

Coenzyme A contains B5, which is ____ and an active ___ group

Answer 13

pantothenic acid; thiol

Question 14

There is 1 subunit of E__, 1 subunit of E__, 2 subunits of E__

Answer 14

1; 3; 2

Question 15

what is the overall rxn?

Answer 15

glucose–>2 pyruvate–>2 Acetyl CoA+2NADH+2CO2

Question 16

what are the inhibitors of this complex?

Answer 16

Acetyl coA, NADH, ATP

Question 17

what are the activators?

Answer 17

pyruvate and ADP

Question 18

the phosphorylated form of PDH is ___, the unphosphorylated form is __

Answer 18

inactive; active