Citric Acid Cycle

Question 1

what is substrate channeling?

Answer 1

process of transferring the product of one enzyme to an adjacent cascade enzyme or cell without complete mixing with the bulk phase

Question 2

what is the 4C acceptor?

Answer 2

oxaloacetate

Question 3

every cycle produces energy equivalent to ___ ATP

Answer 3

10

Question 4

citric acid cycle happens in the ___ while ETC happen in ____

Answer 4

matrix; inner mito membrane

Question 5

Acetyl CoA is made from ______; it is involved both anabolically and catabolically

Answer 5

fa, glucose, amino acids

Question 6

___ produced in Kreb’s while __ is produced in ox phosphorylation

Answer 6

CO2; H2O

Question 7

apples have lots of ____

Answer 7

malate

Question 8

which two molecules have 3 carboxylate groups?

Answer 8

citrate and isocitrate

Question 9

succinate dehydrogenase is associated with _____ of mitochondria, and also component in ___

Answer 9

inner membrane; ETC

Question 10

the enzyme that catalyzes condensation of acetyl CoA and oxaloacetate to form citrate

Answer 10

citrate synthase (you also need water)

Question 11

what is a synthase?

Answer 11

enzyme that catalyzes synthetic rxn which 2 units joined without direct participation of ATP

Question 12

what enzyme catalyzes the form of isocitrate from citrate?

Answer 12

aconitase

Question 13

isocitrate dehydrogenase turns isocitrate into ____ in an ___ ____ reaction

Answer 13

alpha ketoglutarate; oxidative decarboxylation (2 steps)

Question 14

This enzyme catalyzes synth of ______ from alpha ketoglutarate

Answer 14

alpha ketoglutarate dehydrogenase complex; succinyl CoA

Question 15

first place of substrate lvl phosphorylation?

Answer 15

succinyl coA–>succinate

Question 16

succinate–>fumarate enzyme is:

Answer 16

succinate dehydrogenase

Question 17

fumarate–>malate enzyme:

Answer 17

fumarase (H2O added in)

Question 18

malate–>oxaloacetate enzyme:

Answer 18

malate dehydrogenase

Question 19

alpha ketoglutarate dehydrogenase complex is similar to:

Answer 19

PDH complex

Question 20

inhibitor of succinate dehydrogenase

Answer 20

malonate

Question 21

mainly inhibited at ____ dehydrogenase and ____ by ____

Answer 21

isocitrate; alpha ketoglutarate dehydrogenase complex; NADH, ATP

Question 22

replenishing rxns are called:

Answer 22

anaplerotic rxns

Question 23

what is the main anaplerotic rxn catalyzed by?

Answer 23

pyruvate carboxylase (also in gluconeogenesis)

Question 24

cycle similar to CAC but doesn’t exist in animals; it bypasses the 2 decarboxylation steps to allow the synth of ___ from ____

Answer 24

glyoxylate cycle;carbs; fats

Question 25

what is the overall rxn of glyoxylate?

Answer 25

2AcetylCoA+NAD+2H2O–>succinate+2CoASH+NADH+2H