Citric Acid Cycle Flashcards
what is substrate channeling?
process of transferring the product of one enzyme to an adjacent cascade enzyme or cell without complete mixing with the bulk phase
what is the 4C acceptor?
oxaloacetate
every cycle produces energy equivalent to ___ ATP
10
citric acid cycle happens in the ___ while ETC happen in ____
matrix; inner mito membrane
Acetyl CoA is made from ______; it is involved both anabolically and catabolically
fa, glucose, amino acids
___ produced in Kreb’s while __ is produced in ox phosphorylation
CO2; H2O
apples have lots of ____
malate
which two molecules have 3 carboxylate groups?
citrate and isocitrate
succinate dehydrogenase is associated with _____ of mitochondria, and also component in ___
inner membrane; ETC
the enzyme that catalyzes condensation of acetyl CoA and oxaloacetate to form citrate
citrate synthase (you also need water)
what is a synthase?
enzyme that catalyzes synthetic rxn which 2 units joined without direct participation of ATP
what enzyme catalyzes the form of isocitrate from citrate?
aconitase
isocitrate dehydrogenase turns isocitrate into ____ in an ___ ____ reaction
alpha ketoglutarate; oxidative decarboxylation (2 steps)
This enzyme catalyzes synth of ______ from alpha ketoglutarate
alpha ketoglutarate dehydrogenase complex; succinyl CoA
first place of substrate lvl phosphorylation?
succinyl coA–>succinate
succinate–>fumarate enzyme is:
succinate dehydrogenase
fumarate–>malate enzyme:
fumarase (H2O added in)
malate–>oxaloacetate enzyme:
malate dehydrogenase
alpha ketoglutarate dehydrogenase complex is similar to:
PDH complex
inhibitor of succinate dehydrogenase
malonate
mainly inhibited at ____ dehydrogenase and ____ by ____
isocitrate; alpha ketoglutarate dehydrogenase complex; NADH, ATP
replenishing rxns are called:
anaplerotic rxns
what is the main anaplerotic rxn catalyzed by?
pyruvate carboxylase (also in gluconeogenesis)
cycle similar to CAC but doesn’t exist in animals; it bypasses the 2 decarboxylation steps to allow the synth of ___ from ____
glyoxylate cycle;carbs; fats
what is the overall rxn of glyoxylate?
2AcetylCoA+NAD+2H2O–>succinate+2CoASH+NADH+2H
