Protozoa: Apicomplexa - Eimeria, Cryptosporidium

Question 1

Taxa:

Phylum,subphylum, class, order, family

Answer 1

Phylum: Alveolata
Subphylum: Apicomplexa (syn. Protozoa)
Class: Coccidea
- Order: Adeleida
- Family: Haemogregarinidae (Haemogregarina)
Karyolisidae (Karyolysus)
Hepatozoidae (Hepatozoon)
Klossiellidae (Klosiella)

Order: Eimeriida
- Family: Eimeriidae, Sarcocystidae

Order: Cryptosporida
- Family: Cryptosporidiidae (Cryptosporidium)

Question 2

Name species of Eimeriidae:

C,C, E, G, I, T, W

Answer 2

Caryospora, Cyclospora, Eimeria, Goussia, Isospora, Tyzzeria, Wenyonella

Question 3

Name species of Sarcocystidae:

B,C, H, N, S, T

Answer 3

Besnoita, Cystoisospora, Hammondia, Neospora, Sarcocystis, Toxoplasma

Question 4

Diagnosis:

Answer 4

Flotation technique to detect oocyst

Question 5

Morphology of Eimeria:

Answer 5

4 sporocysts with 2 sporozoites

Question 6

Characterize Eimeria and phases of lifecycle:

Answer 6

Host specific
Lifecycle in one host (monoxenous)
3 phases of lifecycle:
1. Sporogony - exogenous
2. merogony - endogenous
3. gamogony - endogenous

Question 7

Name species of Eimeria in chicken:

Answer 7

E. tenella
E. necatrix
E. acervulina
E. maxima
E. brunetti
E. mitis
E. hagani
E. praecox

Question 8

Name species of Eimeria in turkey:

Answer 8

E. adenoides
E. meleagrimitis
E. meleagridis
E. dispersa

Question 9

Name Eimeria in ducks and geese:

Answer 9

E. anseris
E. nocens
E. truncata (kidney)
E. danailovi
E. perniciosa

Question 10

Name Eimeria in pigeons:

Answer 10

E. columbarum
E. labbeana

Question 11

Name Eimeria in rabbit and hare:

Answer 11

Eimeria stiedai (epithelium of bile duct)
- Rabbit: E. magna, E. intestinalis
- Hare: E. europaea

Question 12

Name Eimeria in horses:

Answer 12

E. leuckarti

Question 13

Name EImeria in cattle:

Answer 13

E. zuernii, E. bovis, E. elipsoidalis

Question 14

Name Eimeria in sheep:

Answer 14

E. intricata, E. ovina, E. parva, E. ovinoidalis, E. ninakohlyakimovae, E. caproovina, E. arlonigi, E. caprina, E. christenseni

Question 15

Name EImeria in mice:

Answer 15

E. falciparum, E. ferrisi

Question 16

Name Eimeria in pigs:

Answer 16

E. debliecki, E. neodebliecki, E. scabra, E. suis

Question 17

Name Eimeria in rats:.

Answer 17

E. contorta
E. nieschulzi

Question 18

Name species of genus Cryptosporidium:

Answer 18

C. parvum, C. muris, C. baylei, C. meleagridis

Question 19

Characterize Cryptosporidium:

Answer 19

No host specificity
No micropyle and sporocyst
OOcyst contains 4 free sporozoites
Endogenous sporolation
Microgametes have no flagellum

Question 20

Diagnosis of Cryptosporidium:

Answer 20

DIrect fecal smear (glycerine, water)
Stained fecal smear (carbolfuchsin = carbo-rosaniline stain), Kinyoun stain, Ziehl-Neelsen stain
Detection of coproantigen (rapid test, ELISA)
PCR