Protein Synthesis - Ribosomes and tRNAs

Question 1

Amino acylated tRNAs first bind to small or big subunit?

Answer 1

Small

Question 2

Ribosome
RNA and protein

Answer 2

65% rna

35% protein

Question 3

Bacterial subunits

Eukaryotic subunits

Answer 3

30S 50S —> 70S

40S 60S —> 80S

Question 4

What is in active site of ribosome?

Answer 4

RNA

Ribosome is ribozyme

Question 5

Difference of ribosome assembly between eukaryotes and bacteria?

Answer 5

Subunits in eukaryotes start in nucleolus and need aid in being transported to cytoplasm (snoRNAs)

Question 6

tRNA details

Answer 6

Single strand RNA
73-93 nucleotides
Cells need min 32

Majority have Guanylate on 5’
100% have CCA (3’) at 3’ end
= UGG

Question 7

Clover structure of tRNA starting at top

Answer 7

Amino acid arm
T fork C arm
Extra arm
Anticodon arm
D arm

Question 8

L shape 3d structure tRNA from top

Answer 8

Amino acid arm
T fork arm
D arm
Anticodon arm

Question 9

T fork C arm

Answer 9

Ribothymide
Pseudoridine
Cytidine residues
Important for overall fold in g

Interacts w Large rRNA subunit or ribosome.