Lecture 4 + 5

Question 1

Lesion vs base pair

Answer 1

Issue vs permanent damage

Question 2

Mismatch repair

Answer 2

Strand discrimination via methylated adenine on parent strand

Mut s = scans for lesion

Mut L = finds Gatc with s help = complex creation needs atp

Mut H : snipping = needs atp

Exonuclease unravels

Pol III and ligaments close up

Question 3

Telomeres

Answer 3

Tg strand longer than ca complement

Telomerase adds on telomeres

Question 4

Telomerase

Answer 4

Contains rna and protein components

Internal rna = ca rna strand
5-3 direction

Internal rna ends are complement to its own primer = sliding mechanism

Question 5

Telomerase rna strand in book

Answer 5

AACcccaac

Primer= ttg

Question 6

T Loop

Answer 6

DNA binding proteins
Telomeres repeat binding factors protecting telomere ends

Question 7

RNA dependent polymerase

Answer 7

RNA replicase

5-3

Almost indentical to dna polymerase but does not accept dna