protein synthesis

Question 1

streptomycin what does it affect

what type of antibiotic

Answer 1

gram -ve (tuberculosis)
bactericidal
binds to 30s

Question 2

what does streptomycin cause

Answer 2

accumulation of 70s monomers

frozen in initiation complex

Question 3

where is streptomycin resistant and what protein was mapped

Answer 3

30s resistant and was mapped onto the S12,
lys42 to arg, thr, asp
or lys 87 to arg

Question 4

what does streptomycin induce

Answer 4

it induces error translation

binds to decoding centre increasing the number of wrong tRNAs which cause a conformational change

Question 5

what is streptomycins selective toxicity

Answer 5

does not bind to 80s ribosomes

potent against gram -ve in conjunction with Beta lactams to increase permiability

Question 6

resistance of streptomycin

Answer 6

modified enzymes causing acetylation
altered ribosomes - 1 aa change causes resistance
decreased uptake

Question 7

side effects of streptomycin

Answer 7

muscle weakness- decrease release of Ach
binds to Ca2+ site on the pre synaptic terminal
reversible ototoxicity