Protein Purification

Question 1

What are the intrinsic biological properties by which things can be separated?

Answer 1

Size
Mass
Shape
Interactions
Charge
Hydrophobicity

Question 2

What is salting-out?

Answer 2

Basic and rough separation
Based on the ionic strength of the solution
Separation by size and surface charge distribution
The proteins will precipitate out of the solution

Question 3

What salt is used in salting-out?

Answer 3

Ammonium sulfate (NH4)2SO4

Question 4

Why is ammonium sulfate used in salting-out?

Answer 4

Cheap
Does not buffer
Highly soluble
Low-density solution

Question 5

What is chromatography?

Answer 5

The separation of proteins in a solution

Question 6

What are the different types of protein chromatography?

Answer 6

Affinity
Ion exchange
Size exclusion
Hydrophobic interaction
Reversed-phase
Multimodal chromatography