Fluorescence Flashcards
1
Q
Wha is fluorescence?
A
The emission of photons by matter after absorption of a photon of higher energy
2
Q
What is GFP?
A
Green fluorescent protein
Has a bimodal excitation-emission spectrum
3
Q
What is iLOV?
A
A fluorescent protein with a Flavin mononucleotide cofactor
Engineered from a plant phototropin protein
Doesn’t need oxygen to produce mature chromophore
4
Q
Describe fluorescence polarisation
A
When a fluorophore tumbling in a solution is illuminated by polarised light, the emitted light has a degree of polarisation that is inversely proportional to the speed of rotation
If there is a fast rotation then there is high depolarisation and vice versa
