Circular dichroism Flashcards
1
Q
Describe circular dichroism?
A
Optically active molecules interact with circularly polarised light to change the speed between left and right polarised, their wavelength and absorption
2
Q
Equation for changes in changes in left and right polarisations
A
ΔE=EL=ER
3
Q
Are biological polymers inherently chiral?
A
Yes
4
Q
How are proteins chiral?
A
Have a primary and secondary structure which is chiral
5
Q
How are nucleic acids chiral?
A
Have a helix structure
6
Q
What are the limitations of CD?
A
Buffers can interact with the light
The amide signal in far UV~190nm
7
Q
What are some incompatible solutes for buffers for CD?
A
Phosphate
Sulfate
Ammonium
Carbonate
