FRET Flashcards
1
Q
What does FRET stand for?
A
Forster resonance energy transfer
2
Q
How does a donor fluorophore transfer energy to the acceptor?
A
Through non-radiative dipole-dipole coupling
3
Q
What is the efficiency inversely proportional to?
A
The sixth power of the distance between the donor and the acceptor
4
Q
Describe fluorescence quenching?
A
There is FRET between a fluorescent probe and a quencher
5
Q
What quencher can be used?
A
TaqMan
6
Q
What does TaqMan do?
A
As it extends the primer and bumps into the probe the probe will degrade and release the fluorophore
