Prokaryotic Transcription

Question 1

pol binds to promoter sequence in a duplex, this is called the – complex

Answer 1

closed

Question 2

pol melts duplex DNA near the transcription start site, forming a –

Answer 2

transcription bubble (open complex)

Question 3

transcription bubble is ~ 13 bp:

Answer 3

-11 to + 2

Question 4

pol catalyzes – of two initial rNTPs

Answer 4

phosphodiester linkage

Question 5

during – pol advances 3’ –> 5’ down template strand, melting duplex DNA and adding rNTPS to growing RNA

Answer 5

elongation

Question 6

at transcription stop site, pol – and dissociates from DNA

Answer 6

releases completed RNA

Question 7

Rho-independent transcription termination: intrinsic terminators, meaning that they

Answer 7

recognize their own sequence

Question 8

terminator usually a – sequence followed by an A-rich sequence

Answer 8

G/C rich

Question 9

after transcribing the terminator sequence, the a – forms

Answer 9

RNA:RNA stem loop

Question 10

RNA:RNA stem loops is – due to the G/C rich in stem

Answer 10

stable

Question 11

after the stem loop forms, a – forms via A-U pairing

Answer 11

DNA:RNA hybrid

Question 12

RNA chain is released since – is less stable and is easily dissociated

Answer 12

A-U base pairing

Question 13

prokaryotic RNA pol has exonuclease?

Answer 13

no

Question 14

Prokaryotic RNA pol is composed of 5 subunits:

Answer 14

αI, αII, β, β’ and ω (Core enzyme)

Question 15

core enzyme + sigma factor =

Answer 15

RNA pol holoenzyme

Question 16

– recognizes and binds to the promoter region at -35 and -10

Answer 16

sigma factor 70

Question 17

transcription is inefficient for about the first – NT

Answer 17

10